These technical notes cover hardware related aspects related of our microscope products such as the MicroTime 200 or the LSM Upgrade Kit. This includes, for example, combinations of the MicroTime 200 with AFMs or spectrographs, as well as more detailed descriptions of upgrades of specifc LSMs.
Crossing the Border Towards Deep UV – Time-resolved Microscopy of Native Fluorophores
The technical note describes, how deep UV excitation and detection can be implemented into the time-resolved microscope MicroTime 200. This UV upgrade grants direct access to the native fluorescence of biomolecules originating from naturally occurring chromophoric groups such as tyrosine or tryptophane. Furthermore, some examples including a novel approach based on a combined microchip electrophoresis with deep UV-FLIM are shown.
Combining the MicroTime 200 with the Bruker BioScope Catalyst AFM for Multiparameter Cell Imaging
This technical note describes the realization of the combination between a commercially available AFM (BioScope Catalyst AFM from Bruker) and the time-resolved confocal fluorescence microscope MicroTime 200. The synchronization and alignment scheme as well as first results are described.
Resolve Time and Spectral Information Simultaneously: Spectrograph Add-on for the MicroTime 200
This technical note describes the combination of the MicroTime 200 with the spectrograph SR-163 equipped with a Newton 970 EMCCD camera from Andor Technology. This combination allows to simultaneously record intensity fluctuations, fluorescence decay properties and spectral data of the sample and opens up new prospects in the investigations of nanostructures and single molecules.
Compact FLIM NDD Upgrade Kit for the Olympus FluoView FV1000MPE
The technical note describes the necessary technical modification of the Olympus NDD detection unit in order to use the internal PMTs of the FV1000MPE for FLIM imaging. The potential of this set-up for deep-tissue FLIM imaging is explained as well as all technical aspects that lead to a high system integration of the NDD FLIM upgrade in a FV1000MPE. In addition, the performance of the set-up is demonstrated by a FLIM-FRET experiment of a FRET positive cell.
Combining the MicroTime 200 with the MFP-3D BIO AFM from Asylum Research
This technical note describes the realization of the combination between a commercially available AFM (MFP-3D BIO from Asylum Research) and the time-resolved confocal fluorescence microscope MicroTime 200. The synchronization and alignment scheme as well as first results are described.
Combination of Pulsed and CW Lasers on an Olympus FluoView FV1000 or FluoView FV300
The technical note describes how the simultaneous use of pulsed (PicoQuant) and CW (Olympus) lasers is realized in the Olympus FluoView FV300 and FV1000. All technical aspects of this special coupling via the IR port using a polarization beam splitter are listed.
Polarization Extension Unit for LSM Upgrade Kits
This technical note describes an extension to the LSM upgrade kit that accesses the polarization information for e.g. anisotropy measurements. The extension is based on a polarization sensitive beam splitter that is mounted directly at the LSM exit port. The technical note gives technical details and shows first results on ordered and disordered phases of Giant Unilamellar Vesicles (GUVs) as well as protein oligomerization on a plasma membrane.
Two-photon Excitation Using the MicroTime 200
The application note describes the powerful fusion of fluorescence lifetime imaging microscopy with two-photon excitation (2P-FLIM) using the PicoQuant time-resolved confocal fluorescence microscope MicroTime 200. It shows the experimental setup and performance of the system and presents two examples of quantitative monitoring the intracellular pH and chloride ion concentrations.