NovaFLIM

Fluorescence Lifetime Imaging Analysis Software

Advanced FLIM analysis software for fast, accurate interpretation of lifetime imaging data.
NovaFLIM software interface for fluorescence lifetime imaging analysis

Key Features

Fast GPU-accelerated analysis of FLIM, FLIM-FRET, and anisotropy data
FLIM analysis modes: Fast Lifetime, decay fitting, phasor plots, and pattern matching
Efficient batch analysis of z-stacks, time-lapse series, and stitched images
Advanced, flexible ROI handling and data export options
Reproducible ROI selection using histograms of fitted parameters

Unified Software for FLIM Data Analysis

Analyze fluorescence lifetime data with intuitive visualization tools, including phasor plots, decay fitting, and multi-parameter FLIM maps.

From Lifetime Data to Quantitative Insight

NovaFLIM is a dedicated software platform for fast and reliable analysis of fluorescence lifetime imaging (FLIM) data. It streamlines the evaluation of complex microscopy datasets through GPU-accelerated processing, intuitive visualization tools, and advanced methods such as phasor plots and multi-exponential fitting. Designed for seamless use with Luminosa, MicroTime 200, and PicoQuant’s LSM Upgrade Kit, it supports diverse FLIM, FRET, and anisotropy workflows, delivering clearer insights, higher productivity, and results you can trust.

NovaFLIM Analysis Methods

NovaFLIM support a group of methods for analyzing FLIM images, enabling users to interpret molecular behavior, species distributions, and interactions with confidence.

  • Phasor plot analysis for intuitive lifetime visualization
  • Multi-exponential decay fitting for quantitative lifetime extraction
  • Pattern matching for species separation and multiplexing
  • FLIM-FRET analysis for interaction and energy-transfer studies
  • Anisotropy imaging for molecular orientation and dynamics
  • In combination with NovaISM, ISM-FLIM support (with PDA-23) for resolution-enhanced lifetime imaging
Luminosa confocal microscope combined with NovaFLIM enables advanced FLIM imaging and analysis workflows.

Three Forces. One Vision. Sharper Truth in Every Pixel.

Together with the NovaISM analysis software and the Luminosa single-photon counting microscope, NovaFLIM unlocks advanced FLIM capabilities such as pixel-reassigned ISM-FLIM, computational sectioning , and resolution-enhanced lifetime imaging, creating a powerful analysis environment across PicoQuant’s imaging platforms.

Highlight Benefits

Benefit: Precision

Quality and Precision

Gain an holistic view of your FLIM data by combining multi-exponential fitting, phasor plots, and pattern matching, while enabling quantitative, robust, and reproducible ROI selection via 1D and 2D histograms or directly from the phasor plot.
Benefit: Time

Save Time, Focus on Samples

NovaFLIM delivers high-speed performance through GPU-based algorithms and requires minimal user interaction. It enables efficient analysis of series measurements, including batch processing and parameter plots across multiple ROIs.
Benefits: Fexibility

Advanced Flexibility

Perform simultaneous batch analysis for FLIM, FLIM-FRET, and anisotropy across user-defined channels, with flexible data export options for graphs and images ensuring seamless integration into your analysis workflows and easy sharing of results.

NovaFLIM: Fluorescence Lifetime Imaging Analysis Software

Advanced FLIM analysis software for fast, accurate interpretation of lifetime imaging data.
Relevant for Your Research​

Matching Applications & Methods​

Switcher A&M
Concept illustration of optical environmental sensing where time-resolved fluorescence lifetime detection monitors dynamic environmental signals and trace compounds.
Life Science | Materials Science | Metrology
In-Depth Scientific Resources

Scientific Resources

Access in-depth application notes and scientific posters with detailed methods, measurement data, and real-world use cases.

Poster: New Analysis Options for FLIM Imaging Modalities

Poster on advanced FLIM analysis in NovaFLIM and NovaISM, enabling GPU-accelerated fitting, phasor plots and ISM-FLIM for higher resolution

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Specifications

Licence typeLicense available for either 1, 2, or 4 years
Data formatsAvailable for *.spqr files and *.ptu files acquired with the PDA-23 SPAD Array Detector in the Luminosa confocal microscope
MinimalRecommended
Operating systemWin10 64-bitWin10 / Win11 64-bit
CPUIntel Core i3-8100 or AMD Ryzen 3 1300XIntel Core i7-13700 or AMD Ryzen 7 5700X
RAM8 GB32 GB
GPUAMD Radeon RX550/550 Series or Nvidia GeForce GT 1030AMD Radeon RX 6700 XT or Nvidia GeForce RTX 3060
Screen resolutionQHD (2560 × 1440)4K (3840 × 2160)

Version Features

Luminosa

NovaFLIM

NovaISM

NovaFLIM+NovaISM

InstaFLIM

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Phasor plot for marker multiplexing

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Multi-frame analysis

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Batch analysis of series measurements (time-lapse,z-stacks, tiling, …)

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Advanced ROI handling, multi-ROIs, import of external ROIs

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Parameter plots of fitted parameters over multiple ROIs

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Representation of the image information as user defined 1D and 2D histograms of fitted parameters

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ROI selection via parameter histograms

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User configured analyses allowing for FLIM, FLIM-FRET, steady-state FRET and anisotropy imaging

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Extended export options of graphs and images

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Image Scanning Microscopy-FLIM (Resolution enhancement via a pixel reassigned image available in combination with PDA-23 Add-on)

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Re-assignement vectors calculation from image

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Resolution enhancement Deconvolution for ISM-FLIM (enhanced FLIM contrast via the rejection of contribution from out-of.plane light; Available in combi with PDA-23 Add-on)

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Computational Sectioning in combination with lifetime-species seperation (enhanced FLIM contrast via the rejection of contribution from out-of.plane light; Available in combi with PDA-23 Add-on)

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Technical Documentation and Data

Technical Downloads

Technical Note: Metabolic State Profiling of Organoids with FLIM

Demonstrating metabolic state profiling of organoids using FLIM of NAD(H) autofluorescence with the Luminosa confocal microscope.

Expand Your System

Complete your setup

Combine compatible components to build a complete setup tailored to your experimental requirements and measurement workflows.

Single Photon Counting Confocal Microscope
Reliable single-molecule precision
Time-saving workflows
Fast learning for non-expert users
Flexible for custom experimental setups
Quick results through automated analysis
Expert-level application support
Image Scanning Microscopy Analysis Software
Sharper images with higher resolution
Reliable results with transparent processes
Enhanced contrast in lifetime determination
Improved image contrast through out-of-focus rejection
Reproducible ROI selection using histograms of fitted parameters
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Contact us

Please fill out the form below to request more information and prices about our product. Our team will be happy to review your request and get in touch with you. If additional information is needed to process your inquiry, we will let you know.

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