EasyTau 2

Using EasyTau 2, complex TRES experiments can be performed efficiently through automated acquisition and global analysis workflows. In this example, a tryptophan solution in saline buffer was excited with a pulsed LED, and 31 decay curves were recorded automatically. Global analysis in EasyTau 2 shows that the data are well described by three lifetimes of 360 ps, 2.5 ns, and 7.4 ns.

In this example, the emission anisotropy decay of coumarin 6 in ethylene glycol was recorded at various temperatures. As expected, the model of a single, spherical rotating particle with monoexponential lifetime can be perfectly fitted to the experimental data. The fluorescence lifetime shows only a slight temperature dependence, while the temperature induced viscosity as well as rotational energy changes can be perfectly fitted to the observed rotational correlation times.

Luminescence quantum yields are essential for understanding excited-state behavior and environmental interactions. Using EasyTau 2 in combination with an integrating sphere, absolute quantum yields of liquids and solids can be determined through a guided, step-by-step workflow. A dedicated application wizard simplifies data acquisition and analysis, ensuring reproducible and reliable results.

Using EasyTau 2, fluorescence lifetimes shorter than the instrumental response function can be reliably extracted by accurate reconvolution fitting. In this example, a 15 ps lifetime was obtained from solvent relaxation, despite an IRF width of 55 ps.