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rapidFLIMHiRes

Visualize dynamic processes with ultra fast FLIM imaging and outstanding 10 ps time resolution

 

Interested in ultra fast FLIM imaging with 10 ps time resolution? Try PicoQuant’s rapidFLIMHiRes approach, which redefines Fluorescence Lifetime Imaging (FLIM) by combining fast data acquisition with a 10 ps time resolution for reliable, quantitative analysis. Increased data acquisition speed without sacrifices in lifetime resolution allows studying fast processes such as protein interactions, fast changes in environmental parameters, metabolic imaging, or high throughput FLIM screening.

Check out our white paper and upcoming webinar for more insights into rapidFLIMHiRes.
 
rapidFLIMHiRes is available for PicoQuants's LSM Upgrade Kits and as a special configuration for the time-resolved fluorescence micrsocope MicroTime 200.
Applications rapidFLIM HiRes
 

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Free Webinar

Join our webinar about rapidFLIMHiRes on October 1, 2020 to learn more about this exciting imaging method. PicoQuant's application specialist Maria Loidolt-Krüger will showcase the latest hardware and software developments that allow for rapidFLIM data acquisition and quantitative data analysis. Learn how this method can help you in visualizing dynamic processes in cells or tissues.
Webinar rapidFLIM HiRes
 
 

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White Paper

Free white paper: “Visualize dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding time resolution”. The white paper provides an outline of the general principles underlying rapidFLIMHiRes as well as a series of application examples highlighting the usefulness of this method. A quick run-down of the hardware requirements for implementing rapidFLIMHiRes rounds this application note out.
FLIM-FRET imaging of a CHO cell
  
 

Application Examples

 
 
 
 
Intracellular Ca2+ signals induced by mechanical stimulation of HEK-cells loaded with Oregon-Green-Bapta-1. In this video, the top two cells were mechanically stimulated which leads to a change in fluorescence lifetime.
Sample and data courtesy of M.Sc. Jan Meyer and Prof. Dr. Christine R. Rose (Institut fuer Neurobiologie, HHU Duesseldorf)
Monitoring changes in membrane tension with rapidFLIMHiRes in MDCK cells undergoing osmotic shock. The cell membranes contain a fluorescent tension reporting probe (FliptR). Upon undergoing osmotic shock, a rapid reduction in fluorescence lifetime of Flipper-TR® can be observed.
 
 

New Generation of LSM Upgrade Kits

LSM Upgrade Kit
PicoQuant‘s LSM Upgrade Kits now include all components needed for performing rapidFLIMHiRes: a MultiHarp 150 4P TCSPC unit, appropriate detectors from the PMA Hybrid Series, and the SymPhoTime 64 system software. Check out the LSM Upgrade Kit product page for more details.
 
  

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Contact

If you have any questions concerning rapidFLIMHiRes, please contact us at info@picoquant.com.
 
 
 

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