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Book on Advanced Photon Counting

A book on Advanced Photon Counting, edited by PicoQuant scientists, has been published by Springer in May 2015. The book contains comprehensive reviews, written by experts and is richly illustrated. It is available directly from Springer as part of the "Springer Series on Fluorescence".

Advanced Photon Counting
Applications, Methods, Instrumentation

Editors: Peter Kapusta, Michael Wahl, Rainer Erdmann

Advanced photon countingThis volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Table of contents

  • Modern TCSPC Electronics: Principles and Acquisition Modes
    Michael Wahl
  • Single-Photon Counting Detectors for the Visible Range Between 300 and 1,000 nm
    Andreas Bülter
  • Single-Photon Detectors for Infrared Wavelengths in the Range 1–1.7 µm.
    Gerald S. Buller and Robert J. Collins
  • Modern Pulsed Diode Laser Sources for Time-Correlated Photon Counting
    Thomas Schönau, Sina Riecke, Andreas Bülter, and Kristian Lauritsen
  • Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS
    Thomas Dertinger and Steffen Rüttinger
  • Lifetime-Weighted FCS and 2D FLCS: Advanced Application of Time-Tagged TCSPC
    Kunihiko Ishii, Takuhiro Otosu, and Tahei Tahara
  • MFD-PIE and PIE-FI: Ways to Extract More Information with TCSPC
    Anders Barth, Lena Voith von Voithenberg, and Don C. Lamb
  • Photon Antibunching in Single Molecule Fluorescence Spectroscopy
    Kristin S. Grußmayer and Dirk-Peter Herten
  • FLIM Strategies for Intracellular Sensing
    Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, and Angel Orte
  • Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue
    Rafal Fudala, Ryan M. Rich, Joe Kimball, Ignacy Gryczynski, Sangram Raut, Julian Borejdo, Dorota L. Stankowska, Raghu R. Krishnamoorthy, Karol Gryczynski, Badri P. Maliwal, and Zygmunt Gryczynski
  • Pattern-Based Linear Unmixing for Efficient and Reliable Analysis of Multicomponent TCSPC Data
    Ingo Gregor and Matthias Patting
  • Metal-Induced Energy Transfer
    Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, and Alexey I. Chizhik
  • The Importance of Photon Arrival Times in STED Microscopy
    Giuseppe Vicidomini, Ivan Coto Hernandez, Alberto Diaspro, Silvia Galiani, and Christian Eggeling
  • Single-Color Centers in Diamond as Single-Photon Sources and Quantum Sensors
    Boris Naydenov and Fedor Jelezko
  • Photon Counting and Timing in Quantum Optics Experiments
    Andreas Ahlrichs, Benjamin Sprenger, and Oliver Benson
  • Photon Counting in Diffuse Optical Imaging
    Dirk Grosenick

More details including abstracts and previews are available on the dedicated website at Springer.