EasyTau 2

The EasyTau 2 software is an intuitive though powerful tool to acquire and analyze fluorescence spectra and lifetime decays obtained from FluoTime 300 and FluoTime 250 spectrometers. It can also be used as merely an analysis add-on software for home-built set-ups using PicoQuant TCSPC electronics through importing ASCII or phu files. EasyTau 2 combines easy-to-use algorithms with efficient data management namely tree-structure that makes data dependencies visible at first glance. 

The software is also equipped with a powerful report generator to create presentation-ready plots or tables as PDF or HTML files.

Wide range of measurements and analysis

A wide range of measurements and analysis can be carried out using FluoTime spectrometers and single graphical user interface (GUI) including

• Steady-state emission and excitation spectra
• Fluorescence and phosphorescence lifetime decay
• Time-Resolved Photoluminescence (TRPL)
• Fluorescence anisotropy
• Absolute quantum yield
• Time-Resolved Emission Spectra (TRES)
• Excitation-Emission Matrix (EEM) 
• Chromaticity plot option for electroluminescence measurements (see video >)

Operation modes

A unique and powerful feature of EasyTau 2 software is its three modes of operation that enable users to employ PicoQuant fluorescence spectrometers, FluoTime 300 and FluoTime 250, according to their working routines and requirements.

Wizard mode provides step by step assistance, guides users through all the optimization steps and facilitates beginners to perform various complex measurements.

Customized mode allows full control over the instrumental parameters. It is an ideal mode for advanced users who would like to precisely adjust the measurement criteria. 

Scripting mode allows automation of measurement routines which is especially useful for complex applications. Additionally, EasyTau 2 provides an interface for remote execution of scripts, so that the automation can be extended to third-party accessories such as robotic autosamplers.

Versatile decay analysis

EasyTau 2 supports both tail fitting and numerical reconvolution. The latter is to account for the Instrument Response Function (IRF). The decay data can be fitted with various models including exponential decay (up to 5th order) and distribution models such as Gaussian, Lorenzian, and stretched exponential. All models support both global and batch mode fitting.

Current software version: 2.3

The latest version now supports PicoHarp 330, Prima and Taiko PDL M1 as excitation sources for FluoTime spectrometers as well as switching between different laser drivers at run time. There are also general improvements in the measurement und analysis scripts and a new chromaticity plot option. Note: the software is protected by a USB dongle and will not work without it. Please contact PicoQuant if you want to update EasyTau version 1.x or FluoFit to the latest version.

• Download EasyTau 2.3

Course on time-resolved fluorescence

PicoQuant annually holds the course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy". The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. The course is held in cooperation with Prof. J.R. Lakowicz from the Center of Fluorescence Spectroscopy (CFS) in Baltimore and consists of lectures as well as instrumentation and software hands-on training.

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

The following recorded videos give a demonstration of the powerful options.  

The following documents are available for download:

• Datasheet EasyTau 2
• Brochure about PicoQuant's photon counting and timing products
• Brochure about PicoQuant's FluoTime Series