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Fluorescence Course

20th International Course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”

Berlin, November 12 – 15, 2024

Image Fluorescence Course


Zygmunt 'Karol' Gryczynski during a practical session at the Time-resolved Fluorescence CourseThe course on “Principles and Applications of Time-resolved Fluorescence Spectroscopy” was originally devised by Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy and the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA back in the late 1990s. It has been organized by PicoQuant in Berlin since 2004 and aims at giving you an in-depth introduction into the fascinating world of time-resolved fluorescence spectroscopy techniques and even in combination with microscopy.

Our focus is not limited to applications in Life Sciences but also extends to various topics in Materials Science. This course combines a series of lectures presented by renowned experts in the field with several practical sessions. During these session, we will demonstrate how even novice users can get really great results through a series of live demonstrations on various systems.

Impressions from trf course 2021 - DecayWho should attend?

Our course is aimed at students and researchers who are interested in getting deeper insights into various spectroscopy and microscopy techniques, which are based on time-resolved fluorescence. The course is also a great opportunity to directly interact with renowned specialists as well as for networking with your peers.

Why should you attend?

By joining our event, you will:

  • Learn the basic principles underlying the techniques of time-resolved fluorescence spectroscopy as well as in combination with microscopy
  • Get to know new applications which will be helpful in advance your research
  • Understand how time-resolved data are acquired and can be analyzed
  • Interact directly with experts to discuss your research, daily challenges with samples, and results
  • See different systems featuring various accessories for a broad range of different application and samples
  • Network and socialize with your fellow scientists and foster collaborations

Which topics are covered?

Attending all lectures and practical sessions will improve your understanding of the following topics:

  • Instrumentation and data analysis
  • Time-resolved measurements from ps up to ms of liquids and solids
  • Anisotropy, TRES, Excitation Emission Map, Upconversion
  • TRPL imaging: coupling of a photoluminescence (PL) spectrometer with a microscope
  • Imaging methods: FLIM and  PLIM

Practical sessions

A series oif practical sessions will complement the course's lectures. In these sessions, we will demonstrate a broad range of time-resolved spectroscopy and microscopy experiments on various state-of-the-art instruments and software solutions.

Participants about the course

Group picture 2021"Access to top experts in the field."

"All presentations were of a high quality and gave very good basic explanations about fluorescence or new insights on the subject!"

"It is very complete. You explain lots of differents techniques and instruments."

"Great people and moderator."

Information and correspondence

Tel: +49-30-1208820-87
Fax: +49-30-1208820-90
Email: trf@picoquant.com

Please note: schedule and content of this event is subject to change without notice.

Course instructors

  • Zygmunt Gryczynski Zygmunt "Karol" Gryczynski
    Department of Physics and Astronomy at the Texas Christian University, Fort Worth, Texas, USA
  • Ute Resch-Genger Ute Resch-Genger
    Federal Institute for Materials Research and Testing (BAM), Berlin, Germany
  • Andreas BülterAndreas Bülter
    PicoQuant GmbH, Berlin, Germany
  • Christian OelsnerChristian Oelsner
    PicoQuant GmbH, Berlin, Germany
  • Olaf SchulzOlaf Schulz
    PicoQuant GmbH, Berlin, Germany
  • Martin HofMartin Hof
    J. Heyrovsky Institute of Physical Chemistry, Prague, Czech Republic
  • Christian WürthChristian Würth
    Federal Institute for Materials Research and Testing (BAM), Berlin, Germany
  • Eugeny ErmilovEugeny Ermilov
    PicoQuant GmbH, Berlin, Germany
  • Matthias PattingMatthias Patting
    PicoQuant GmbH, Berlin, Germany
  • Michael Wahl
    PicoQuant GmbH, Berlin, Germany


All times given in CEST (Central European Summer Time).

Prelimenary course schedule:

Tuesday, November 12
Wednesday, November 13
Thursday, November14
Friday, November15
Zygmunt “Karol” Gryczynski  
FAQ - Answering questions from participants
Zygmunt “Karol” Gryczynski
FAQ - Answering questions from participants
Andreas Bülter
Welcome & Introduction
Zygmunt “Karol” Gryczynski
Basic Definitions and Principles of Fluorescence
Zygmunt “Karol” Gryczynski
Biophysical Applications and Time-resolved Fluorescence
Zygmunt “Karol” Gryczynski
Advanced Fluorescence Application
Olaf Schulz
Introduction to Fluorescence Microscopy Techniques
Coffee Break
Coffee Break
Coffee Break
Change Buildings and Coffee Break
Zygmunt “Karol” Gryczynski
Time-resolved Fluorescence and Fluorescence Probes
Ute Resch-Genger
Christian Würth
Time-resolved Fluorescence for Materials Science
Matthias Patting
Computational Aspects of TCSPC Data Analysis
Practical sessions (5)
Group Picture
Lunch Break
Lunch Break
Lunch Break
Lunch Break and End of Course
Olaf Schulz
Instrumentation for Time-resolved Fluorescence Measurements – General Overview
Christian Oelsner
Eugeny Ermilov

Practical aspects of time-resolved fluorescence measurements in Materials Science
Martin Hof
Advances of Time-resolved fluorescence spectroscopy in membrane and enzyme studies
FAQ- Answering questions/ customer measurements
Coffee Break
Coffee Break
Change Buildings
Matthias Patting
Practical TCSPC Data Analysis
Michael Wahl
Instrumentation for Time-resolved Fluorescence Measurements (2)
Practical Sessions (3)
Andreas Bülter
Introduction to Practical Sessions
Change Buildings
Coffee Break
Change Buildings
Practical Sessions (2)
Practical Session (4)
16:45 - 18:15
Practical Sessions (1)
Welcome Reception

Download program > 


Please select the appropriate pricing for your institution.
How did you hear about the course?
Terms and Conditions
Privacy Policy

All the information provided during the registration process will be used by PicoQuant for organizing the event and contacting consented participants exclusively about matters related to the fluorescence course. Any information you provide will not be sold, rented, leased or forwarded to any third party.

Personal data

What is your scientific background?

Please provide short answers to the following questions, which help us in fine-tuning the course. Thank you.

Please provide your personal level of knowledge about Time-Resolved Fluorescence
In which area of scientific research are you working?
Do you use any of the following methods in your research: steady-state fluorescence, time-resolved fluorescence, anisotropy, fluorescence lifetime imaging ? If yes, please name which.
Are you already working with PicoQuant systems?
What are your expectations/what would you like to learn during the course?

Course fees

  until July 15 July 16 - October 15
Academic/University 700 € 850 €
Industry and Private Sector 1350 € 1650 €

Besides full course attendance, the fee includes coffee breaks, a reception with food and drinks, and four lunches. Attendees will be responsible for their own travel, lodging, and additional meals.



We have negotiated special rates for a limited number of rooms located close to the workshop location. The number of rooms as well as booking time are limited and we therefore advise to reserve your room as soon as possible.

AirporthotelAirporthotel Berlin-Adlershof
Rudower Chaussee 14
12489 Berlin

Room prices per night:

  • Single room: 109 € including breakfast
  • Double room: 139 € including breakfast

Booking Code: This information will be sent with your registration confirmation. If you require this information at an earlier point, please contact us.

Adapt ApartmentsAdapt Apartments - Berlin Adlershof
Erich-Thilo-Straße 3
12489 Berlin

Prices per night:

Single Apartments: 107,10 € without breakfast

Booking Code: This information will be sent with your registration confirmation. If you require this information at an earlier point, please contact us.

Further hotels within walking distance to the workshop location:

Essential by Dorint Hotel Berlin-Adlershof


Terms and Conditions

Payment and Cancellations

  • After online registration, you will receive an email notification including a PDF file that includes information on the payment procedure.
  • All payments have to be received within 14 days after date of registration.
  • We will send an email confirming your participation once we have received your payment. If payment is overdue, your registration will not be processed and will be considered invalid.
  • A payment receipt will be included in our email confirmation of participation.
  • Cancellation of registration must be submitted in writing or via email and is valid only with acknowledgment of receipt by PicoQuant GmbH. A refund of registration fees is dependent on the notice given:
    • For cancellations made until October 8, 2024, 75 % of the received registration fee will be reimbursed. In case of cancellations after October 8, 2024, 25 % of the registration fee will be reimbursed.
    • It is possible to name and send a substitute participant.
  • No visa letters will be issued until payment of the registration fee is received and confirmed.


  • Schedule and content of this event are subject to change without notice.
  • PicoQuant may record photographs and video material of participants at the event. By registering for a PicoQuant event, you agree that we may include images of yourself (either as a full image or in parts) for promotional purposes in various publications (press releases, promotional activities, event website, etc.). This material may also appear on our internet web page or on related social media pages. If you do not want us to use pictures in which you appear, please inform us in written form until the end of the course.
  • We may also share personal information of event attendees (e.g. in a contact list) with the other persons attending the same event to enable attendees to contact each other prior to, during or after the event for networking purposes. 

Cancellation of registration by PicoQuant due to force majeure

  • Any circumstances beyond the reasonable control of PicoQuant, including but not limited to acts of nature, fire, severe thunderstorm, earthquakes, volcanic eruptions, terror, threats, pandemics, local or international health crises, security/safety risks or flooding shall constitute “force majeure”. If the force majeure is affecting a PicoQuant event, PicoQuant will notify the participants of such event without undue delay.
  • When PicoQuant is subject to force majeure, it shall be released from the performance of its obligations relating to any events (partially or wholly) to the extent that such performance is affected by the force majeure.
  • PicoQuant may take reasonable steps to minimize the consequences of the force majeure on its events and is entitled to reschedule or shorten (in all cases partially or wholly) an event and any performance related to such event. 
  • For termination of contract concerning an event between PicoQuant and the participant due to force majeure, this shall be without any liability for either party, except in so far as has accrued prior to the date on which the termination of the contract takes effect.
  • Each party to the contract concerning the event (PicoQuant and the participant) shall itself be responsible for all direct and indirect financial consequences affecting it as a result of or in connection with the force majeure. The occurrence of force majeure shall not entitle either party to any additional payment or compensation.

Code of conduct

The open exchange of ideas and freedom of thought and expression across different fields of research are central to the aims and goals of the Time-resolved Fluorescence Spectroscopy Course. These require an environment that recognizes the inherent worth of every person and group, that fosters dignity, understanding, mutual respect, and embraces diversity. For these reasons, the Time-resolved Microscopy Course organizers are committed to providing a harassment-free course experience.

If you experience harassment or discriminatory behavior at the Time-resolved Fluorescence Spectroscopy Course, we encourage you to reach out to us for help.

Participants who violate this code may be sanctioned and/or expelled from the event, at the discretion of the organizers. Serious incidents may be referred to the Time-resolved Fluorescence Spectroscopy Course organizers for further possible action.

If you witness harassment or discriminatory behavior, please consider intervening.

Rights releases

To encourage open communication, each member of the Time-resolved Fluorescence Spectroscopy Course agrees that any information presented at the course, whether in a formal talk or discussion, is a private communication from the individual making the contribution and is presented with the restriction that such information is not for public use.  

Prior to quoting or publishing such information presented at a conference in any publication, written or electronic, written approval of the contributing member must first be obtained.  The audio or video recording of lectures by any means, the photography of slide, and printed or electronic quotes from papers, presentations and discussion at a conference without written consent of the contributing member is prohibited.  Scientific publications are not to be prepared as emanating from the conferences.  Authors are requested to omit references to the conferences in any publication, written or electronic.  These restrictions apply to each member of a conference and are intended to cover social networks, blogs, tweets or any other publication, distribution, communication or sharing of information presented or discussed at the conference. 

Guests are not permitted to attend the conference lectures.  Each member of a conference acknowledges and agrees to these restrictions when registration is accepted and as a condition of being permitted to attend a conference. 

Although PicoQuant staff will take reasonable steps to enforce the restrictions against recording and photographing conference presentations, each member of a conference assumes sole responsibility for the protection and preservation of any intellectual property rights in such member's contributions to a conference.



The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.



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