Fluorescence Course
18th International Course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”
November 2020 in Berlin, Germany
This course is held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy and the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Aim and purpose
The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Materials and Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, individuals from totally different research areas and from industry get the opportunity to enter this exciting field in a very effective way and will especially benefit from the experimental section.
Course organization
The course consists of lectures and practical hands-on sessions include steady-state and time-resolved experiments as well as data analysis, performed on various instruments provided by market leading companies.
The lectures on Monday and Tuesday will deal with basics of steady-state and time-resolved fluorescence spectroscopy, instrumentation and data analysis. They cover time-domain and frequency-domain measurements, anisotropy, solvent effects, quenching and Förster Resonance Energy Transfer (FRET). Moreover, applications of time-resolved luminescence spectroscopy in biophysics and in the materials science will be discussed.
On Wednesday and Thursday, the lectures cover advanced time-resolved fluorescence topics and data analysis. Plasmon controlled fluorescence or material science along with an introduction to fluorescence microscopy will be presented.
The practical sessions including steady-state and time-resolved experimentation as well as data analysis are offered on various instruments provided by market leading companies.
Practical sessions
Practical sessions on various instruments will be offered. A detailed overview will be published in April 2020.
A detailed overview will be published at a later date.
The following companies will offer practical sessions:
- Cary Eclipse - a steady-state fluorescence spectrophotometer
- Cary 3500 - a steady-state UV-Vis spectrophotometer
- FluoTime 250 - a compact and modular fluorescence lifetime spectrometer
- FluoTime 300 - an automated fluorescence lifetime spectrometer
- EasyTau 2 - a global fluorescence decay data analysis software
- MicroTime 100 - a compact confocal microscope for FLIM, time-resolved photoluminescence and screening
- MicroTime 200 - a flexible time-resolved confocal fluorescence microscope with unique single molecule sensitivity
Important dates
Important dates will be published in April 2020
- Final deadline for course registration: September 24, 2019
- Deadline for early bird registration: July 31, 2019
- Deadline for fee waver application: July 31, 2019
Information and correspondence
Course coordinator: Lukas Hager
Tel: +49-30-1208820-87
Fax: +49-30-1208820-90
Email: trf@picoquant.com
Please note: schedule and content of this event is subject to change without notice.
Course instructors
The list of speakers will be announced in April 2020.
Joseph R. Lakowicz
Professor of Biochemistry and Director of
the Center for Fluorescence Spectroscopy
Zygmunt "Karol" Gryczynski
Professor at Department of Physics and Astronomy at the Texas Christian University
Nikolai Tkachenko
Professor of Physical Chemistry at Tampere University
Rainer Erdmann
Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
Evangelos Sisamakis
Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
Matthias Patting
Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
Michael Wahl
Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)
The list of instructors is subject to changes.
Time schedule
The schedule will be published in May 2020.
The time schedule of the course is also available for download.
| Monday, November 4 |
Tuesday, November 5 |
Wednesday, November 6 |
Thursday, November 7 |
|---|---|---|---|
| 8:00 Registration |
|||
| 8:45 Rainer Erdmann Welcome and Introduction |
8:15 Joseph R. Lakowicz Zygmunt “Karol” Gryczynski FAQ - Answering questions from participants |
8:15 Joseph R. Lakowicz Zygmunt “Karol” Gryczynski FAQ - Answering questions from participants |
|
| 9:00 Joseph R. Lakowicz Basic Definitions and Principles of Fluorescence
|
9:00 Joseph R. Lakowicz Biophysical Applications and Time-resolved Fluorescence |
9:00 Joseph R. Lakowicz Advanced Topic: Plasmon-controlled Fluorescence |
9:00 Evangelos Sisamakis Introduction to Fluorescence Microscopy Techniques |
| 10:30 Coffee Break |
10:30 Coffee Break |
10:30 Coffee Break |
10:45 Change Buildings and Coffee Break |
| 11:00 Joseph R. Lakowicz Time-resolved Fluorescence and Fluorescence Probes |
11:00
Nikolai Tkachenko Time-resolved luminescence applications in materials science |
11:00 Matthias Patting Computational Aspects of TCSPC Data Analysis |
11:15 Practical Sessions (5) |
| 12:30 Group Picture |
|||
| 12:45 Lunch Break |
12:30 Lunch Break |
12:30 Lunch Break |
12:45 Lunch Break and End of Course |
| 13:45 Rainer Erdmann Instrumentation for Time-Resolved Fluorescence Measurements (1) |
13:30 Zygmunt “Karol” Gryczynski Fluorescence Measurements, Probes & Standards |
13:30 Zygmunt “Karol” Gryczynski Analytical Applications of Fluorescence |
13:45-15:15 FAQ - Answering questions/customer measurements |
| 15:00 Coffee Break |
15:00 Coffee Break |
15:00 Change Buildings |
|
| 15:30 Matthias Patting Practical TCSPC Data Analysis |
15:30 Michael Wahl Instrumentation for Time-resolved Fluorescence Measurements (2) |
15:15 Practical Sessions (3) |
|
| 16:00 Introduction to Practical Sessions |
|||
| 16:30 Change Buildings |
16:30 Change Buildings |
16:45 Coffee Break |
|
| 16:45-18:15 Practical Sessions (1) |
16:45-18:00 Practical Sessions (2) |
17:00 - 18:30 Practical Sessions (4) |
|
| 19:00 Reception |
19:45 Course Dinner |
The time schedule is subject to changes.
Registration
Registration is closed. If you are still interested we can put you on our waiting list. Please send an e-mail on trfcourse@picoquant.com.
Registration opens in June 2020.
Archive
The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.
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