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Fluorescence Course

17th European Short Course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”

November 4-7, 2019 in Berlin, Germany

Image Fluorescence Course 2019

This course is held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy and the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Aim and purpose

The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, individuals from totally different research areas and from industry get the opportunity to enter this exciting field in a very effective way and will especially benefit from the experimental section.

Course organization

Lecture of J.R. LakowiczThe course consists of lectures and experimentations include steady-state and time-resolved experiments as well as data analysis, performed on various instruments provided by market leading companies.

The lectures on Monday and Tuesday will deal with basics of steady-state and time-resolved fluorescence spectroscopy, instrumentation and data analysis. They cover time-domain and frequency-domain measurements, anisotropy, solvent effects, quenching and Förster Resonance Energy Transfer (FRET).

On Wednesday and Thursday, the lectures cover advanced time-resolved fluorescence topics and data analysis. Applications of fluorescence in biophysics, sensing, plasmon controlled fluorescence or material science are presented along with an introduction to fluorescence microscopy.

The practical sessions including steady-state and time-resolved experimentation as well as data analysis are offered on various instruments provided by market leading companies.

Practical Sessions

Practical sessions on various instruments will be offered. A detailed overview will be published at a later date.

Practical Session on an FluoTime 200The following companies will offer practical sessions:

  • Cary Eclipse - a steady-state fluorescence spectrophotometer
  • Cary 60 - a steady-state UV-Vis spectrophotometer

  • FluoTime 300 - an automated fluorescence lifetime spectrometer
  • EasyTau 2 - a global fluorescence decay data analysis software
  • MicroTime 100 - a compact confocal microscope for FLIM, time-resolved photoluminescence and screening
  • MicroTime 200 - a flexible time-resolved confocal fluorescence microscope with unique single molecule sensitivity

Important dates

  • Deadline for early bird registration: July 31, 2019
  • Final deadline for course registration: September 24, 2019
  • Deadline for fee waver application: July 31, 2019

Information and correspondence

Course coordinator: Andrea Usison

Tel: +49-30-1208820-645
Fax: +49-30-1208820-90
Email: trfcourse@picoquant.com

Please note: schedule and content of this event is subject to change without notice.

Course instructors

  • Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
  • Evangelos Sisamakis, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
  • Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
  • Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)

The list of instructors is subject to changes.

The list of speakers will be announced in April 2019.

Time schedule

The schedule will be published in April 2019.


The time schedule of the course is also available for download.

November 5
November 6
November 7
November 8
Joseph R. Lakowicz
Zygmunt “Karol” Gryczynski
FAQ - Answering questions from participants
Joseph R. Lakowicz
Zygmunt “Karol” Gryczynski

FAQ - Answering questions from participants
Rainer Erdmann
Welcome and Introduction
Joseph R. Lakowicz
Biophysical Applications and Time-resolved Fluorescence
Joseph R. Lakowicz
Advanced Topic: Controlling Fluorescence with Plasmonic and Photonic Nanostructures
Evangelos Sisamakis
Introduction to Fluorescence Microscopy Techniques
Joseph R. Lakowicz
Basic Definitions and Principles of Fluorescence



Coffee Break


Coffee Break


Change Buildings and Coffee Break


Coffee Break


Michael Wahl

Instrumentation for Time-resolved Fluorescence Measurements (2)


Matthias Patting

Computational Aspects of TCSPC Data Analysis


Practical Sessions (5)


Joseph R. Lakowicz

Time-resolved Fluorescence and Fluorescence Probes


Lunch Break


Lunch Break


Lunch Break and End of Course


Group Picture


Matthias Patting

Practical TCSPC Data Analysis


Zygmunt “Karol” Gryczynski

Analytical Applications of Fluorescence




Lunch Break


Andreas Bülter

Introduction to Practical Sessions


Change Buildings



Rainer Erdmann

Instrumentation for Time-Resolved Fluorescence Measurements (1)


Change Buildings


Practical Sessions (3)



Coffee Break


Practical Sessions (1)


Coffee Break


15:45 - 17:30

Zygmunt “Karol” Gryczynski

Fluorescence Measurements, Probes & Standards


Coffee Break

17:00 - 18:30

Practical Sessions (4)



Opening Reception

16:30 - 18:00

Practical Sessions (2)


Course Dinner



Registration opens in June 2019.

Registration is closed. If you are still interested we can put you on our waiting list. Please send an e-mail on trfcourse@picoquant.com.

Course fees

The course fees will be announced in June 2019.

  before July 30 August 1 until September 24
Academic / University 700 € 850 €
Industry and Private Sector 1400 € 1600 €

Besides full course attendance, the fee includes all coffee breaks, 1 reception, 1 dinner, daily lunch, course materials and the use of instruments. Attendees will be responsible for their own travel, lodging and meals.

Please note the terms and conditions.

  1. For payment you can choose between credit card (Visa, Master Card) and bank transfer. Possible bank charges have to be paid by the participant. Please note, that we do not accept checks.
  2. After online registration, you will receive an email notification including a PDF file that includes information on the payment procedure.
  3. All payments have to be received within 14 days after date of registration.
  4. We will send an email confirming your participation once we have received your payment. If payment is overdue, your registration will not be processed and considered invalid.
  5. A receipt of payment will be included in our email confirmation of participation.
  6. Cancellation of registration must be submitted in writing or via email and is valid only with acknowledgment of receipt by PicoQuant GmbH. A refund of registration fees is dependent on the notice given:
    • For cancellations made until September 24, 2018, 75 % of the received registration fee will be reimbursed.
    • In case of cancellations after September 24, 2018, 25 % of the registration fee will be reimbursed.
    • It is possible to name and send a substitute participant.
  7. No visa letters will be issued until payment of the registration fee is received and confirmed.
  8. Schedule and content of this event is subject to change without notice.
  9. PicoQuant records photographs and video material of participants at the events. By registering for a PicoQuant event, you agree that we may include images of yourself (either as a full image or in parts) for promotional purposes (press releases, event website, etc.). If you do not want us to use pictures in which you appear, please inform us in written form.


Financial support

Details on how to apply for a fee waiver will be announced at a later date.

As in the previous years, PicoQuant will grant a fee waiver to a few participants from the university and academic sector of economically less privileged countries. Accomodation, travel and personal expenses still need to be paid by the participants themselves. The selection of sponsored people is completely the sole decision of PicoQuant and there is no right or guarantee to receive a fee waiver.

To apply for a fee waiver please send us your application including:

  • a letter of application
  • a formal letter of recommendation from your department/institute.

Please note that only one person per research group can be considered for a fee waiver.

Deadline for fee waiver applications is July 30, 2018.

The deadline to apply for a fee waiver has passed. We can no longer accept applications.



We will negotiate special rates for the course participants with some hotels. The contact information and prices will be listed on this website in June 2019.

We have negotiated special rates for a limited number of rooms in two local hotels which are in walking distance to the course location. Please note that the number of rooms and the booking time are limited and we therefore advise to reserve your room as soon as possible.

City Tax

Please note that since the beginning of the year 2014, tourists staying overnight in Berlin are subject to paying an accommodation tax, the so-called City Tax. It amounts to five percent of the room rate (net price), excluding VAT and fees for amenities and services such as mini-bar, sauna, or spa area. The City Tax does only affect private overnight stays and NOT business travellers. The business purpose of a trip can be verified by a bill that is paid by or issued to the employer, or a letter from the company. If the accommodation is booked by the employer in the first place, there is no further proof necessary.

Also see the information at www.berlin.de.

Airporthotel Berlin-Adlershof
Rudower Chaussee 14, 12489 Berlin
Phone: +49-30-720-2222-000
Fax: +49-30-720-2222-100
Website of the hotel

Room prices per night

  • single room: 63 € (excl. breakfast)
  • double room: 78 € (excl. breakfast)
  • breakfast: 13 € per day and person
Airporthotel Berlin-Adlershof

Booking code: PQ-TRF Kurs

Please use the booking form to reserve a room.

The rooms are bookable at this rate until October 8, 2018. We can not guarantee any reservations at these prices or any reservation at all after this date.


ADAPT Apartments
Erich-Thilo-Straße 3, 12489 Berlin
Phone: +49-30-678-929-80
Fax: +49-30-678-929-82
Website of the apartment house

Room prices per night

  • single room: 72 € (excl. breakfast)

Guests can join the breakfast at the ManiMogo for a special price of 10 € per person and day.

Wireless LAN is included in the room price.

ADAPT Apartments Berlin-Adlershof


Booking code: TRF Kurs PQ

Please write an email to info@adaptberlin.de to reserve a room, mentioning the booking code “TRF Kurs PQ”.

The rooms are bookable at this rate until September 24, 2018 on a first come, first served basis.



The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.


Thank you for registering for the Fluorescence Course!

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