Fluorescence Course

This course is held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy and the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA.

Aim and purpose

The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, individuals from totally different research areas and from industry get the opportunity to enter this exciting field in a very effective way and will especially benefit from the experimental section.

Course organization

The course consists of lectures and experimentations include steady-state and time-resolved experiments as well as data analysis, performed on various instruments provided by market leading companies.

The lectures on Monday and Tuesday will deal with basics of steady-state and time-resolved fluorescence spectroscopy, instrumentation and data analysis. They cover time-domain and frequency-domain measurements, anisotropy, solvent effects, quenching and Förster Resonance Energy Transfer (FRET).

On Wednesday and Thursday, the lectures cover advanced time-resolved fluorescence topics and data analysis. Applications of fluorescence in biophysics, sensing, plasmon controlled fluorescence or material science are presented along with an introduction to fluorescence microscopy.

The practical sessions including steady-state and time-resolved experimentation as well as data analysis are offered on various instruments provided by market leading companies.

Practical Sessions

The following companies will offer practical sessions:

Cary Eclipse - a steady-state fluorescence spectrophotometer
Cary 60 - a steady-state UV-Vis spectrophotometer

FluoTime 300 - an automated fluorescence lifetime spectrometer
EasyTau 2 - a global fluorescence decay data analysis software
MicroTime 100 - a compact confocal microscope for FLIM, time-resolved photoluminescence and screening
MicroTime 200 - a flexible time-resolved confocal fluorescence microscope with unique single molecule sensitivity

Important dates

Deadline for early bird registration: July 31, 2018
Final deadline for course registration: September 24, 2018
Deadline for fee waver application: July 31, 2018

Information and correspondence

Course coordinator: Nicola Kasse

Tel: +49-30-1208820-642
Fax: +49-30-1208820-90
Email: trfcourse@picoquant.com

Please note: schedule and content of this event is subject to change without notice.

Course instructors

Joseph R. Lakowicz
Professor of Biochemistry and Director of
the Center for Fluorescence Spectroscopy
Zygmunt "Karol" Gryczynski
Professor at Department of Physics and Astronomy at the Texas Christian University

Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
Evangelos Sisamakis, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)

The list of instructors is subject to changes.

Course location

The 16. course will be held in the "Max-Born-Saal" in Berlin-Adlershof, located in the south-east of Berlin. The local area map below shows the course location (blue marker).

Time schedule

The time schedule of the course is also available for download.

Monday, November 5	Tuesday, November 6	Wednesday, November 7	Thursday, November 8
8:00 Registration	8:15 Joseph R. Lakowicz Zygmunt “Karol” Gryczynski FAQ - Answering questions from participants	8:15 Joseph R. Lakowicz Zygmunt “Karol” Gryczynski FAQ - Answering questions from participants
8:45 Rainer Erdmann Welcome and Introduction	9:00 Joseph R. Lakowicz Biophysical Applications and Time-resolved Fluorescence	9:00 Joseph R. Lakowicz Advanced Topic: Controlling Fluorescence with Plasmonic and Photonic Nanostructures	9:00 Evangelos Sisamakis Introduction to Fluorescence Microscopy Techniques
9:00 Joseph R. Lakowicz Basic Definitions and Principles of Fluorescence	10:30 Coffee Break	10:30 Coffee Break	10:45 Change Buildings and Coffee Break
10:30 Coffee Break	11:00 Michael Wahl Instrumentation for Time-resolved Fluorescence Measurements (2)	11:00 Matthias Patting Computational Aspects of TCSPC Data Analysis	11:15 Practical Sessions (5)
11:00 Joseph R. Lakowicz Time-resolved Fluorescence and Fluorescence Probes	12:30 Lunch Break	12:30 Lunch Break	12:45 Lunch Break and End of Course
12:30 Group Picture	13:30 Matthias Patting Practical TCSPC Data Analysis	13:30 Zygmunt “Karol” Gryczynski Analytical Applications of Fluorescence
12:45 Lunch Break	14:00 Andreas Bülter Introduction to Practical Sessions	15:00 Change Buildings
13:45 Rainer Erdmann Instrumentation for Time-Resolved Fluorescence Measurements (1)	14:30 Change Buildings	15:15 Practical Sessions (3)
15:15 Coffee Break	14:45 Practical Sessions (1)	16:45 Coffee Break
15:45 - 17:30 Zygmunt “Karol” Gryczynski Fluorescence Measurements, Probes & Standards	16:15 Coffee Break	17:00 - 18:30 Practical Sessions (4)
18:30 Opening Reception	16:30 - 18:00 Practical Sessions (2)	19:45 Course Dinner

Registration

Affiliation

First name *

Institution *

Street

ZIP code *

Email *

Last Name *

Department *

City *

Country *

Phone

Scientific background

Questions about scientific background

Please provide a short answer to the following questions, which helps us in fine-tuning the course. Thank you.

(1) What is your educational level and discipline(s)?

(2) What is your experience with steady-state fluorescence (i.e. courses taken, instruments you are familiar with, etc.)?

(3) What is your experience with time-resolved fluorescence or other time-resolved spectroscopies? What instrumentation have you used (e.g. manufacturer, model, etc.)?

(4) What is your goal in attending the course?

Course fees

Please select the appropriate pricing for your institution. *

University / Academic
Industry

Terms and Conditions

Agreement *

Yes, I agree to the following terms and conditions

For payment you can choose between credit card (Visa, Master Card) and bank transfer. Possible bank charges have to be paid by the participant. Please note, that we do not accept checks.
After online registration, you will receive an email notification including a PDF file that includes information on the payment procedure.
All payments have to be received within 14 days after date of registration.
We will send an email confirming your participation once we have received your payment. If payment is overdue, your registration will not be processed and considered invalid.
A receipt of payment will be included in our email confirmation of participation.
Cancellation of registration must be submitted in writing or via email and is valid only with acknowledgment of receipt by PicoQuant GmbH. A refund of registration fees is dependent on the notice given:
- For cancellations made until September 24, 2018, 75 % of the received registration fee will be reimbursed. In case of cancellations after September 24, 2018, 25 % of the registration fee will be reimbursed.
- It is possible to name and send a substitute participant.
No visa letters will be issued until payment of the registration fee is received and confirmed.
Schedule and content of this event is subject to change without notice.
PicoQuant records photographs and video material of participants at the events. By registering for a PicoQuant event, you agree that we may include images of yourself (either as a full image or in parts) for promotional purposes (press releases, event website, etc.). If you do not want us to use pictures in which you appear, please inform us in written form.

Additional Information

How did you hear about the course?

Privacy policy

All the information provided during the registration process will be used by PicoQuant for organizing the event and contacting consented participants exclusively about matters related to the fluorescence course. Any information you provide will not be sold, rented, leased or forwarded to any third party. Please also see our privacy policy.

I have read and accept the privacy policy of PicoQuant.*

I allow my personal data to be included in a participant list that will be distributed to the participants only.

Course fees

	before July 30	August 1 until September 24
Academic / University	700 €	850 €
Industry and Private Sector	1400 €	1600 €

Besides full course attendance, the fee includes all coffee breaks, 1 reception, 1 dinner, daily lunch, course materials and the use of instruments. Attendees will be responsible for their own travel, lodging and meals.

Please note the terms and conditions.

For payment you can choose between credit card (Visa, Master Card) and bank transfer. Possible bank charges have to be paid by the participant. Please note, that we do not accept checks.
After online registration, you will receive an email notification including a PDF file that includes information on the payment procedure.
In order to take advantage of the early bird rate (deadline: July 30, 2018), payments have to be received by August 6, 2018.
All other payments have to be received within 14 days after date of registration.
We will send an email confirming your participation once we have received your payment. If payment is overdue, your registration will not be processed and considered invalid.
A receipt of payment will be included in our email confirmation of participation.
Cancellation of registration must be submitted in writing or via email and is valid only with acknowledgment of receipt by PicoQuant GmbH. A refund of registration fees is dependent on the notice given:
- For cancellations made until September 24, 2018, 75 % of the received registration fee will be reimbursed.
- In case of cancellations after September 24, 2018, 25 % of the registration fee will be reimbursed.
- It is possible to name and send a substitute participant.
No visa letters will be issued until payment of the registration fee is received and confirmed.
Schedule and content of this event is subject to change without notice.
PicoQuant records photographs and video material of participants at the events. By registering for a PicoQuant event, you agree that we may include images of yourself (either as a full image or in parts) for promotional purposes (press releases, event website, etc.). If you do not want us to use pictures in which you appear, please inform us in written form.

Financial support

As in the previous years, PicoQuant will grant a fee waiver to a few participants from the university and academic sector of economically less privileged countries. Accomodation, travel and personal expenses still need to be paid by the participants themselves. The selection of sponsored people is completely the sole decision of PicoQuant and there is no right or guarantee to receive a fee waiver.

To apply for a fee waiver please send us your application including:

a letter of application
a formal letter of recommendation from your department/institute.

Please note that only one person per research group can be considered for a fee waiver.

Deadline for fee waiver applications is July 30, 2018.

Accommodation

We have negotiated special rates for a limited number of rooms in two local hotels which are in walking distance to the course location. Please note that the number of rooms and the booking time are limited and we therefore advise to reserve your room as soon as possible.

City Tax

Please note that since the beginning of the year 2014, tourists staying overnight in Berlin are subject to paying an accommodation tax, the so-called City Tax. It amounts to five percent of the room rate (net price), excluding VAT and fees for amenities and services such as mini-bar, sauna, or spa area. The City Tax does only affect private overnight stays and NOT business travellers. The business purpose of a trip can be verified by a bill that is paid by or issued to the employer, or a letter from the company. If the accommodation is booked by the employer in the first place, there is no further proof necessary.

Also see the information at www.berlin.de.

Airporthotel Berlin-Adlershof
Rudower Chaussee 14, 12489 Berlin
Phone: +49-30-720-2222-000
Fax: +49-30-720-2222-100
Website of the hotel
reservierung@airporthotel-berlin-adlershof.de

Room prices per night

single room: 63 € (excl. breakfast)
double room: 78 € (excl. breakfast)
breakfast: 13 € per day and person

Booking code: PQ-TRF Kurs

Please use the booking form to reserve a room.

The rooms are bookable at this rate until October 8, 2018. We can not guarantee any reservations at these prices or any reservation at all after this date.

ADAPT Apartments
Erich-Thilo-Straße 3, 12489 Berlin
Phone: +49-30-678-929-80
Fax: +49-30-678-929-82
Website of the apartment house
info@adaptberlin.de

Room prices per night

single room: 72 € (excl. breakfast)

Guests can join the breakfast at the ManiMogo for a special price of 10 € per person and day.

Wireless LAN is included in the room price.

Booking code: TRF Kurs PQ

Please write an email to info@adaptberlin.de to reserve a room, mentioning the booking code "TRF Kurs PQ".

The rooms are bookable at this rate until September 24, 2018 on a first come, first served basis.