Time-resolved Microscopy Course
14th International Course on “Time-resolved Microscopy and Correlation Spectroscopy”
Spring 2022 in Berlin, Germany
Scope
Our course provides an in-depth introduction to the world of time-resolved fluorescence microscopy with a focus on life science applications. The course combines a series of lectures by experts in the field with practical sessions on systems from market-leading companies.
Who should attend?
The content is geared towards both novice researchers as well as those having some previous experience with time-resolved methods and applications.
Why should you attend?
Join our event to:
- Learn the principles behind time-resolved fluorescence microscopy
- Get to know powerful applications that will help advance your research
- Understand how time-resolved data is acquired and analyzed
- Interact directly with experts to discuss your research and foster collaborations
- See confocal microscopes from various companies live in action
- Talk to and socialize with your fellow scientists
And all of this from the safety and comfort of your home.
Which topics are covered?
Attending the lectures and practical sessions will provide you with a better understanding of the following topics:
- Instrumentation and data analysis
- Correlation spectroscopy methods: FCS, FCCS, FLCS, ...
- Imaging methods: FLIM, rapidFLIM, FRET, FLIM-FRET, ...
- Single molecule microscopy
Practical sessions
Confocal microscopes from major manufacturers will be used during the practical sessions. A detailed overview will be published at a later date
On each day we will perform a live demonstration on one of the systems that focuses on a special topic (see schedule). This will give everyone a chance to familiarize themselves with all featured instruments and techniques and ask live questions during these sessions!
The following instruments will be featured:
- Nikon: A1R_HD25
- Olympus: FluoView FV3000
- Zeiss: LSM 980
- PicoQuant: MicroTime 200 with FLIMbee
Important dates
- Deadline for early bird registration: December 31, 2020
- Final deadline for course registration: January 31, 2021
SymPhoTime Training Day
The course will be accompanied by PicoQuant’s “SymPhoTime Training Day”, a one day virtual meeting for users of the SymPhoTime and SymPhoTime 64 software packages.
The exact meeting date will be announced later.
One day after the course, on February 21, 2020, PicoQuant will host the “SymPhoTime Training Day” for users of the SymPhoTime and SymPhoTime 64 software.
For details visit the event website.
Participants about the course
“I really like the structure of the Microscopy Course, offering a mix of basic lectures, advanced applications, and practical methods. A major highlight was the great interaction with the audience”
Dr. Michael Ebner, Invited Speaker 2021
Contact
Course coordinator: Marta Kolonko
Tel: +49-30-1208820-87
Fax: +49-30-128820-90
Email: trfcourse@picoquant.com
Please note: schedule and content of this event are subject to change without notice.
Course instructors
The list of course instructors will ve published at a later date
Alessandro Esposito
University of Cambridge, United Kingdom
Michael Ebner
Leibniz Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany
Jelle Hendrix
Biomedical Research Institute, Hasselt, Belgium
Stefanie Weidtkamp-Peters
Heinrich-Heine-Universität Düsseldorf, Germany
Olaf Schulz
PicoQuant GmbH, Berlin, Germany
Samaneh Rezvani
PicoQuant GmbH, Berlin, Germany
Schedule
Course schedule will be announced at a later date.
Note: All times given here refer to the local time in Berlin (UTC+1)
| Tuesday, March 2 |
Wednesday, March 3 |
Thursday, March 4 |
Friday, March 5 |
|---|---|---|---|
| 9:45 Opening remarks and technical warm-up |
9:50 Welcome |
9:50 Welcome |
9:50 Welcome |
| 10:00 Introduction to FLIM Olaf Schulz PicoQuant, Berlin, Germany |
10:00 Introduction to FCS Olaf Schulz PicoQuant, Berlin, Germany |
10:00 Raster and temporal ICS: principles, novel developments and applications Jelle Hendrix Biomedical Research Institute, Hasselt, Belgium |
10:00 Fast multiplexed biochemical imaging Alessandro Esposito University of Cambridge, UK |
| 10:45 Q&A session with speaker |
10:45 Q&A session with speaker |
10:45 Q&A session with speaker |
10:45 Q&A session with speaker |
| 11:00 Break |
11:00 Break |
11:00 Break |
11:00 Break |
| 11:30 Introduction to FLIM/FRET Samaneh Rezvani PicoQuant, Berlin, Germany |
11:30 Introduction to FLCS, Single Molecule Microscopy Samaneh Rezvani / Olaf Schulz PicoQuant, Berlin, Germany |
11:30 Harnessing the power of F(C)CS to address protein-membrane interactions in living cells Michael Ebner Leibniz Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany |
11:30 Investigating protein-protein interaction in plant tissue using FLIM-FRET Stefanie Weidtkamp-Peters Heinrich-Heine-Universität Düsseldorf, Germany |
| 12:15 Q&A session with speaker |
12:15 Q&A session with speakers |
12:15 Q&A session with speaker |
12:15 Q&A session with speaker |
| 12:30 Break |
12:30 Break |
12:30 Break |
12:30 Break |
| 14:00 Practical session: FLIM basics Nikon |
14:00 Practical session: FCS/SMD Basics MicroTime 200 PicoQuant |
14:00 Practical session: Advanced FCS Zeiss |
14:00 Practical session: Advanced FLIM/FRET Olympus |
| 16:00 Open discussion |
16:00 Open discussion |
16:00 Open discussion |
16:00 Open discussion |
| 16:30 End of day 1 |
16:30 End of day 2 |
16:30 End of day 3 |
16:30 End of course |
Thank you for registering for the Time-resolved Microscopy Course!
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