Time-Resolved Microscopy Course

Aim and purpose

The course is intended for individuals wishing an in-depth introduction to the principles of time-resolved fluorescence microscopy and its applications to the Life Sciences. Attendees are usually professionals who are using or intend to use fluorescence microscopy in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, other individuals from totally different research areas and from industry get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.

Practical sessions

Practical sessions using confocal microscopes provided by several market leading companies are part of the course:

Nikon: A1R_HD25
Olympus: FluoView FV3000
Scientifica: HyperScope
Zeiss: LSM 980
PicoQuant: MicroTime 200 STED with FLIMbee, MicroTime 100 combined with FluoTime 250

Participants will be divided into groups that rotate through the stations. This ensures that every participant will have the chance to familiarize himself with the broad range of techniques and methods of fluorescence (lifetime) microscopy.

Topics covered

Topics covered during the lectures and experimental station include:

Instrumentation and data analysis
Correlation methods: FCS, FCCS, FLCS, ...
Imaging methods: FLIM, rapidFLIM, FRET, FLIM-FRET, ...
2Photon-FLIM
STED super-resolution microscopy
Single molecule microscopy

Important dates

Registration will open mid October 2019
Deadline for early bird registration: November 22, 2019
Deadline for fee waiver applications: November 22, 2019
Final deadline for course registration: January 7, 2020

SymPhoTime Training Day

One day after the course, on February 21, 2020, PicoQuant will host the “SymPhoTime Training Day” for users of the SymPhoTime and SymPhoTime 64 software.

For details visit the event website.

Contact

Course coordinator: Marta Kolonko

Tel: +49-30-1208820-87
Fax: +49-30-128820-90
Email: trfcourse@picoquant.com

Please note: schedule and content of this event are subject to change without notice.

Course instructors

Jan Willem Borst
Wageningen University and Research, The Netherlands
Jörg Enderlein
University of Göttingen, Germany
Fred Wouters
University of Göttingen, Germany

Chris Dunsby
Imperial College London, United Kingdom
Mark Hink
University of Amsterdam, The Netherlands
Andreas Bülter
PicoQuant GmbH, Berlin, Germany

Schedule

The time schedule of the course is also available for download.

Tuesday, February 18	Wednesday, February 19	Thursday, February 20
8:15 Registration	9:00 Mark Hink Introduction to Fluorescence Correlation Spectroscopy	9:00 Jörg Enderlein Single Molecule Spectroscopy and Superresolution Imaging
8:45 Welcome and Introduction	10:30 Coffee Break	10:30 Coffee Break
9:00 Jan Willem Borst Introduction to Microscopy	11:00 Chris Dunsby Fluorescence Lifetime Imaging	11:00 Andreas Bülter Instrumentation for Time-resolved Microscopy
10:30 Coffee Break	12:30 Lunch Break	12:30 Lunch Break
11:00 Fred Wouters (Jan Willem Borst) Förster Resonance Energy Transfer	14:00 Practical Session III	14:00 Practical Session V
12:30 Group Picture and Lunch Break	16:00 Coffee Break	16:00 Coffee Break
14:00 Practical Session I	16:30 Practical Session IV	16:30 Practical Session VI
16:00 Coffee Break	18:30 Spare Time	18:30 End of Course
16:30 Practical Session II
18:45 Opening Reception

Also see our SymPhoTime Training Day that takes place February 21, 2020 in Berlin.

Course fees

	until November 22, 2019	November 23, 2019 until January 17, 2020
Academic/University	600 €	750 €
Industry and Private Sector	1200 €	1500 €

Besides full course attendance, the fee includes all coffee breaks, a reception with free food and drinks, daily lunch, course materials and the use of instruments. Attendees will be responsible for their own travel, lodging and meals.

Please note the terms and conditions.

For payment you can choose between credit card (Visa, Master Card) and bank transfer. Possible bank charges have to be paid by the participant. Please note that we do not accept checks.
After online registration, you will receive an email notification including a PDF file that includes information on the payment procedure.
All payments have to be received within 14 days after date of registration.
We will send an email confirming your participation once we have received your payment. If payment is overdue, your registration will not be processed and considered invalid.
A receipt of payment will be included in our email confirmation of participation.
Cancellation of registration must be submitted in writing or via email and is valid only with acknowledgment of receipt by PicoQuant GmbH. A refund of registration fees is dependent on the notice given:
- For cancellations made until January 17, 2020, 75 % of the received registration fee will be reimbursed. In case of cancellations after January 17, 2020, 25 % of the registration fee will be reimbursed.
- It is possible to name and send a substitute participant.
No visa letters will be issued until payment of the registration fee is received and confirmed.
Schedule and content of this event is subject to change without notice.
PicoQuant records photographs and video material of participants at the events. By registering for a PicoQuant event, you agree that we may include images of yourself (either as a full image or in parts) for promotional purposes (press releases, event website, etc.). If you do not want us to use pictures in which you appear, please inform us in written form.

Financial support

As in the previous years, PicoQuant will grant a fee waiver to a few participants from the university and academic sector of economically less privileged countries. Accommodation, travel and personal expenses still need to be paid by the participants themselves. The selection of sponsored people is completely the sole decision of PicoQuant and there is no right or guarantee to receive a fee waiver.

To apply for a waiver fee, please send us your application per e-mail. The application has to include the following:

a letter of application
a formal letter of recommendation from your department/institute

Deadline for a fee waiver application is November 22, 2019.

Please note that only one person per research group can be considered for a fee waiver.

Accommodation

We have negotiated special rates for a limited number of rooms in two hotels. The number of rooms and the booking time are limited and we therefore advise to reserve your room as soon as possible.

ADAPT Apartments
Erich-Thilo-Straße 3, 12489 Berlin
Phone: +49-30-678-929-80
Fax: +49-30-678-929-82
Website of the apartment house
info@adaptberlin.de

Room prices per night

single room: 72 € (excl. breakfast)
double room: 94.50 € (excl. breakfast)

The prices can slightly change.

Guests can join the breakfast at the ManiMogo for a special price of 10 € per person and day.

Wireless LAN is included in the room price.

Booking code: TRMic 2020

Please make your reservation by phone (+49-30-6789-2980) or by email (info@adaptberlin.de) indicating the booking code.

The rooms are bookable at this rate until February 10, 2020 on a first come, first served basis.

Essential by Dorint Berlin Adlershof
Rudower Chaussee 15, 12489 Berlin
Phone: +49-30-67822-0
Fax: +49-30-67822-1000
Website of the hotel
info.berlin-adlershof@dorint.com

Room prices per night

single room: 91 € (incl. breakfast)
double room: 110 € (incl. breakfast)

These prices are subject to change.

Wireless LAN is included in the room price.

Please use the online booking form to make your reservation.

If you do not wish to have breakfast included, please inform the hotel when making your reservation.

The rooms are bookable at this rate until February 20, 2020 on a first come, first served basis. We cannot guarantee reservations at these prices or any reservations at all after this date.