Fluorescence Course
16th European Short Course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”
November 5-8, 2018 in Berlin, Germany
This course was held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy & the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA
Summary
The 16th “European Short Course on Time-resolved Fluorescence Spectroscopy” held by PicoQuant in Berlin from November 5-8, 2018 gave 40 participants from around the world an in-depth introduction into the principles of this method as well as its applications in research fields such as life sciences. Highlight of the event were a series of lectures by Prof. Joseph R. Lakowicz from the Center of Fluorescence Spectroscopy (CFS, Baltimore, USA) and Prof. Zygmunt “Karol” Gryczynski from the Texas Christian University (Texas, USA). The course program was complemented by practical sessions, a welcoming reception as well as a dinner. The latter two social events gave attendees the opportunity for discussing and networking in an informal atmosphere.
Course organisation
The course opened with a lecture on the principles of fluorescence spectroscopy by Joseph R. Lakowicz and was followed by lectures by Zygmunt “Karol” Gryczynski and Senior Scientists from PicoQuant covering topics like instrumentation for time-resolved fluorescence spectroscopy and microscopy, anisotropy, solvent effects, quenching, Förster Resonance Energy Transfer (FRET) and applications of fluorescence methods in life and material sciences.
The practical sessions were led by application specialists from PicoQuant and Agilent. Each experimental station focused on a specific fluorescence method such as anisotropy, steady-state and time-resolved fluorescence spectroscopy, and data analysis. A short demonstration of Fluorescence Lifetime Imaging (FLIM) and STED super-resolution was also offered. The instruments presented included steady-state (Cary Eclipse, Agilent) and time-resolved spectrometers (FluoTime 250 and FluoTime 300, PicoQuant) as well as a time-resolved super-resolution microscope MicroTime 200 STED from PicoQuant.
Practical sessions
The following companies offered hands-on experiments during the course:
- Agilent
- PicoQuant
Course instructors
Joseph R. Lakowicz
Professor of Biochemistry and Director of
the Center for Fluorescence Spectroscopy
Zygmunt "Karol" Gryczynski
Professor at Department of Physics and Astronomy at the Texas Christian University
- Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
- Evangelos Sisamakis, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
- Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
- Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)
Schedule
The time schedule is still available for download as a pdf document.
Course impressions
Archive
The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.
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