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Fluorescence Course

5th European short course on
"Principles and Applications of Time-resolved Fluorescence Spectroscopy"

October 29 - November 2, 2007 in Berlin, Germany

Image Fluorescence Course 2007

Logo CFSThis course is held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy & the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA


This course was held in Europe for the fifth time and was again a great success. Altogether more than 70 people from universities and industry from 23 different countries participated. They attended 14 lectures and 6 hands-on experiment sessions with 8 different instruments from 4 companies, covering different methods and procedures in fluorescence spectroscopy.

Group picture at the fluorescence course 2007

Aims and Purpose

The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.

Course organisation

The lectures on Monday and Tuesday dealt with basic principles of fluorescence spectroscopy, while from Wednesday to Friday more specialized topics were covered:

Monday and Tuesday - Principles of Fluorescence Spectroscopy

These lectures dealt with the basics of steady-state and time-resolved fluorescence spectroscopy and instrumentation. They covered time- and frequency-domain measurements, anisotropy, solvent effects, quenching and Förster Resonance Energy Transfer (FRET), as well as an introduction into data analysis.

Wednesday to Friday - Time-Resolved Fluorescence Spectroscopy: Principles, Instrumentation and Applications

These lectures emphasised time-resolved fluorescence along with advanced applications of fluorescence in biophysics, sensing, imaging, clinical chemistry, microscopy and ultrasensitive detection. Guest lectures were given on various topics, like fluorescence microscopy, correlation spectroscopy and single molecule detection.

Apart from the lectures, hands-on experimentation (steady-state and time-resolved experiments as well as data analysis) was offered on various instruments provided by market leading companies.

Hands-on instrumentation

The following companies offered hands-on experiments during the course:

  • Olympus
  • ISS
  • PicoQuant
  • Varian

Course instructors

  • Joseph R. Lakowicz, Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy
  • Zygmunt "Karol" Gryczynski, Professor at the Department of Molecular Biology and Immunology at the University of North Texas
  • Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
  • Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for time-correlated single photon counting instrumentation
  • Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for time-correlated single photon counting

Guest lecturers

  • Manfred Auer, Head of Innovative Screening Technologies (IST) at the Novartis Lead Discovery Center, as well as lecturer for Biophysical Chemistry at the University of Salzburg, Austria. He specializes in the development of novel drug discovery technologies.
  • Jörg Enderlein, Professor at the University of Tübingen, Germany. He specializes in Single Molecule Detection, Fluorescence Correlation Spectroscopy (FCS) and complex data analysis.
  • Paul French, Head of the Photonics group at the Imperial College London, UK. He specializes on biomedical optics with particular emphasis on coherence-gated imaging through turbid media and fluorescence lifetime imaging applied to molecular biology, clinical imaging and drug discovery.
  • Cristina Flors, Post-Doc in the single molecule and fluorescence microscopy group in the laboratory for photochemistry and spectroscopy, devision for molecular and nanomaterials, departement for chemistry, KULeuven, Belgium. She is specialized in application of time resolved fluorescence techniques and single molecule spectroscopy in material science and biosciences.


The time schedule is still available for download as a pdf document.

Course impressions



The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.


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