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Fluorescence Course

7th European short course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”

November 9 - 12, 2009 in Berlin, Germany

Image Fluorescence Course 2009

Logo CFSThis course is held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy & the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA


This course was held in Europe for the seventh time and was again an inspiring event. Altogether more than 50 people from universities and industry from 16 different countries participated. They attended 12 lectures and 5 hands-on experiment sessions with 7 different instruments from 3 companies, covering different methods and procedures in fluorescence spectroscopy.

Group picture of the fluorescence course 2009

Aims and Purpose

The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.

Course organisation

The lectures on Monday and Tuesday dealt with basic of steady-state and time-resolved fluorescence spectroscopy, instrumentation and data analysis. They covered time- and frequency-domain measurements, anisotropy, solvent effects, quenching and Förster Resonance Energy Transfer (FRET).

The lectures on Wednesday and Thursday covered advanced time-resolved fluorescence topics and data analysis. Applications of fluorescence in biophysics, sensing, clinical chemistry or material science were presented along with an introduction into fluorescence microscopy.

Apart from the lectures, hands-on experimentation (steady-state and time-resolved experiments as well as data analysis) was offered on various instruments provided by market leading companies.

Hands-on instrumentation

The following companies offered hands-on experiments during the course:

  • Horiba Jobin-Yvon
  • PicoQuant
  • Varian

Course instructors

  • Joseph R. Lakowicz, Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy
  • Zygmunt “Karol” Gryczynski, Professor at the Department of Molecular Biology and Immunology at the University of North Texas
  • Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
  • Felix Koberling, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy
  • Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for time-correlated single photon counting instrumentation
  • Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for time-correlated single photon counting


The time schedule is still available for download as a pdf document.

Course impressions



The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.


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