13th European short course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”
November 2-5, 2015 in Berlin, Germany
This course was held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy & the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA
During the 13th edition of the "European Short Course on Time-resolved Fluorescence", 37 participants from around the world got an in-depth introduction to time-resolved spectroscopy and microscopy in the field of Life Sciences through a series of lectures and hands-on experiments. The course was complemented by a welcoming reception on the first day and a dinner on the third day, giving the attendees the opportunity for meeting the lecturers and networking in an informal atmosphere.
The course opened with a lecture on the principles of fluorescence spectroscopy by Joseph R. Lakowicz and was followed by lectures by Zygmunt “Karol” Gryczynski and Senior Scientists of PicoQuant covering topics like instrumentation for time-resolved fluorescence spectroscopy and microscopy, anisotropy, solvent effects, quenching, Förster Resonance Energy Transfer (FRET) and applications of fluorescence in Life and Material Sciences.
The practical hands-on sessions were led by application specialists from PicoQuant and Agilent. Each experimental station focused on a specific fluorescence method such as anisotropy, steady-state and time-resolved fluorescence spectroscopy, and data analysis. A short demonstration of Fluorescence Lifetime Imaging (FLIM) and STED super-resolution was also offered. The instruments presented included steady-state (Cary Eclipse, Agilent) and time-resolved spectrometers (FluoTime 200 and FluoTime 300, PicoQuant) as well as a time-resolved super-resolution microscope MicroTime 200 STED from PicoQuant.
The following companies offered hands-on experiments during the course:
- Joseph R. Lakowicz, Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy
- Zygmunt "Karol" Gryczynski, Professor at Department of Physics and Astronomy at the Texas Christian University
- Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
- Felix Koberling, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
- Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
- Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)
The time schedule of the course is still available for download.
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