Fluorescence Course
15th European short course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”
November 6-9, 2017 in Berlin, Germany
This course was held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy & the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA
Summary
40 participants from around the world attended the 15. edition of the “European Short Course on Time-resolved Fluorescence Spectroscopy” in Berlin. The course organized by PicoQuant GmbH in cooperation with Joseph R. Lakowicz from the Center of Fluorescence Spectroscopy (CFS) in Baltimore, USA gave an in-depth introduction into the principles of fluorescence spectroscopy as well as its applications to life science. The event consisted of series of lectures and hands-on experiments, complemented by a welcoming reception on the first day and a dinner on the third day. It gave the attendees the opportunity for meeting the lecturers and networking in an informal atmosphere.
Course organisation
The course opened with a lecture on the principles of fluorescence spectroscopy by Joseph R. Lakowicz and was followed by lectures by Zygmunt “Karol” Gryczynski and Senior Scientists of PicoQuant covering topics like instrumentation for time-resolved fluorescence spectroscopy and microscopy, anisotropy, solvent effects, quenching, Förster Resonance Energy Transfer (FRET) and applications of fluorescence in Life and Material Sciences.
The practical hands-on sessions were led by application specialists from PicoQuant and Agilent. Each experimental station focused on a specific fluorescence method such as anisotropy, steady-state and time-resolved fluorescence spectroscopy, and data analysis. A short demonstration of Fluorescence Lifetime Imaging (FLIM) and STED super-resolution was also offered. The instruments presented included steady-state (Cary Eclipse, Agilent) and time-resolved spectrometers (FluoTime 200 and FluoTime 300, PicoQuant) as well as a time-resolved super-resolution microscope MicroTime 200 STED from PicoQuant.
Hands-on instrumentation
The following companies offered hands-on experiments during the course:
- Agilent
- PicoQuant
- Joseph R. Lakowicz, Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy
- Zygmunt "Karol" Gryczynski, Professor at Department of Physics and Astronomy at the Texas Christian University
- Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
- Felix Koberling, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
- Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
- Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)
Schedule
The time schedule is still available for download as a pdf document.
Course impressions
Archive
The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.
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