Fluorescence Spectrometers

FluoTime 300

High-End Photoluminescence Spectrometer

  • Steady-state and time-resolved (TCSPC, MCS) operation mode
  • Highly modular and flexible design, optimal for upgradability
  • Fully automated system with exact timing from ps to s
  • Double or single monochromator in excitation and emission with the unique feature of switching between additive and subtractive in emission
  • Superior sensitivity with > 32 000:1 water Raman SNR
  • Intuitive acquisition and analysis EasyTau 2 software


  • Micro-photoluminescence upgrade for combining microscopy and spectroscopy
  • Integrating sphere for absolute luminescence quantum yield measurement
  • Versatile sample holders for handling liquids and solids
  • NEW: Class 1 laser safety

Sample mounting unit with temperature stabilzed holder for 1x1cm cuvettes inside FluoTime 300The FluoTime 300 is a high performance fluorescence spectrometer for materials science, life science, and photochemistry applications. Our fully automated setup contains  complete optics and electronics for recording steady-state spectra as well as fluorescence decays by means of Time-Correlated Single Photon Counting (TCSPC) or Multichannel Scaling (MCS) from a few picoseconds to several seconds. The FluoTime 300 provides superior sensitivity, spectral, and temporal resolution that allow the acquisition and analysis of

from UV to IR range. This setup can be equipped with numerous sample holders ranging from standard cuvette and front face to wafer check. The sample compartment is designed to be spacious to provide more flexibility. For measurements under controlled temperatures, PicoQuant offers various solutions depending on the temperature range.

All components are being controlled by EasyTau 2 software. A unique feature of PicoQuant spectroscopy software are its three operation modes: 

  • Wizard mode provides step by step assistance
  • Customized mode enables full control over the instrument
  • Scripting mode allows automation of measurement routines.

The FluoTime 300 is an optimal choice not only for routine lab work but also for cutting-edge research fields. Over 450 research articles have been published utilizing the FluoTime 300 in various fields including solar cells, light emitting diodes (LEDs), photocatalysis, upconversion materials, and quantum dot characterization.


Recent publications by researchers using the FluoTime 300


FluoTime Show Day 2021Virtual show day: Closer look at the FluoTime series

Our first FluoTime Show Day was held on June 17, 2021. During this virtual event, an in-depth introduction on PicoQuant spectroscopy products was provided by our application specialists. Request the recording and learn how our products can help you in your daily research tasks to carry out steady-state and time-resolved luminescence measurements with a micrometer sized positionable observation volume.

Time-resolved Fluorescence CourseCourse on time-resolved fluorescence

PicoQuant annually holds the European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy". The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the life sciences. For details see the course website.

Optical configuration
  • L-Geometry
Mode of operation
  • Steady-state
  • Time-Correlated Single Photon Counting (TCSPC)
  • Multichannel Scaling (MCS)
Sensitivity Signal-to-noise ratio (SNR) > 32 000:1 measured using double monochromators in excitation and emission light paths, PMA Hybrid 06 detector
Lifetime range
  • < 10 ps with TCSPC electronics
  • > 5 s with MCS electronics

Depending on light source and detector choice

Excitation sources
  • 255 – 1990 nm picosecond pulsed light sources with repetition rates up to 80 MHz
  • 266 – 590 nm high power UV lasers (VisUV)
  • 200 – 900 nm CW Xenon lamp (300 W)
  • 200 – 900 nm sub-µs pulsed Xenon flash lamp
  • External lasers e.g., Ti:Sapphire, white light lasers
  • Czerny-Turner type
  • Focal length: 300 mm (single, double) and 150 mm (single)
  • Grating*: 
    • 600 g/mm blazed at 1250 nm in emission
    • 1200 g/mm blazed at 500 nm in emission
    • 1200 g/mm blazed at 300 nm in excitation 
  • Dispersion:
    • 5.4 nm/mm (single, 150 nm)
    • 2.7 nm/mm (single, 300 nm)
    • 2.7 nm/mm (double, subtractive)
    • 1.35 nm/mm (double, additive)
  • Stray light rejection: 1:10-5 (single), 1:10-8 (double)

* Others on request

  • 230 – 920 nm: Photomultiplier Tubes (PMTs)
  • 220 – 890 nm : Hybrid-PMTs
  • 950 – 1700 nm: NIR-PMTs
  • Windows based acquisition and analysis software
  • Lifetime analysis based on numerical reconvolution procedure and up to 5th exponential decay functions
  • Lifetime distributions, anisotropy, global analysis, rigorous error analysis
  • Assisted mode using Wizards for standardized measurements
  • Customized mode with full control of all hardware parameters
  • Scripting mode for automation of routine measurements

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

Specialized sample holders

FluoTime 300 - Sample mounting unit with temperature stabilzed holder for 1x1cm cuvettes
Temperature stablized cuvette
10 x 10 mm cuvettes holder for liquid samples that can be connected to an external water bath for temperature stabilization via cable glands at the units front size.

FluoTime 300 - Sample mounting unit with 4-position holder for 1x1cm cuvettes
Peltier-cooled four position cuvette 
Four position Peltier-cooled holder for 10 x 10 mm cuvettes with the temperature range of -15 to 110 C. The temperature is fully controlled by EasyTau 2 software and can thus be automated to carry out measurements at various temperatures.

FluoTime 30ß -Sample mounting unit with front face sample holder
Adjustable front-face
The mounting unit designed for samples sizes of up to two inches. The holder enables accurate adjustments of sample position and angle with respect to the direction of excitation and detection.

FluoTime 300 - Sample mounting unit with fiber coupling sample holder
Integrating sphere
Our designed integrating sphere measures the absolute photoluminescence quantum yield providing high accuracy for both liquid as well as solid samples. We are able to reproduce the published literature data of quantum yield standards such as Rhodamin 6G, Coumarin 153, and Ru(bpy)3.

FluoTime 300 - Sample mounting unit with fiber coupling sample holder
Sample holder with fiber coupler
This unit enables fiber coupling of FluoTime 300 with confocal and widefield optical microscopes. In this way, steady-state and time-resolved luminescence spectra of a sample mounted on microscope can be acquired.

Sample mounting unit for wafers and flat samples
Holder designed for wafers and flat samples with a maximum 2 inches in diameter. The external dials allow for horizontal as well as rotational adjustments.

FluoTime 300 - liquid nitrogen cryostat
Liquid nitrogen
The temperature range of the cryostat is 77 to 500 K. The sample mounting unit is equipped with an adapter to fit to the sample chamber.

FluoTIme 300 - Closed cycle helium cryostat
Closed cycle helium
CS-204 is a compact, axially symmetrical, closed-cycle cryocooler that enables photoluminescence measurements from 4 K to 500 K.

FluoTime 300 - Sample mounting unit for liquid nitrogen dewar
Dewar for measurements at 77 K
The quartz dewar allows access to the sample from four directions. It is an inexpensive alternative to measure at 77 K. The sample is positioned in a quartz sample rod that is submerged in liquid nitrogen.


The FluoMic add-on combining a FluoTime 300 and a microscopeFluoMic add-on: time-resolved and steady-state photoluminescence measurements

The FluoMic add-on for the FluoTime 300 provides a fast and easy way to perform time-resolved as well as steady-state photoluminescence measurements on a wide range of solid objects outside of the spectrometer. The add-on extends the power and capabilities of the FluoTime 300 with the ability to gather data from a well defined observation spot that can be freely placed. This additional spatial information enables you to get a more detailed picture of the processes and dynamics occurring in your sample by letting you know what happens where.

> Check out our white paper which showcases the capabilities of the FluoMic add-on.

> Read about further options of combining spectroscopy and microscopy.



FluoTime 300 coupled to workstation Biomek NXP from Beckman-CoulterAdvanced automation for time-resolved spectroscopy

FluoTime 300 can be coupled to third-party accessories such as the liquid handling automation workstation Biomek NXP from Beckman-Coulter. This combination allows to extend the automation to the loading and removal of samples from the spectrometer, simplifying the work flow for both high-throughput applications and obtaining spectroscopic snap shots at well defined time points.

Check out the demonstration video.

Solar Cells and Photovoltaics

Determining electron-hole diffusion lengths in perovskite solar cells using a FluoTime 300A critical parameter in understanding the photophysics of semiconductor solar cells is the diffusion length of the photo-excited electrons and holes in the material. Time-resolved photoluminescence quenching experiments are a valuable tool for determining diffusion lengths. The example shows data obtained from mixed halide and triiodide organometal perovskite layers in presence of either an electron (blue) or hole (red) quenching layer, or a PMMA coating (black). The decay curves were recorded at 780 nm, corresponding to the peak emission of both materials. The measured decay dynamics can be fitted to a diffusion model, allowing to derive diffusion lengths. Here, the diffusion length of the electrons and holes in the mixed halide perovskite was 1 μm while the triiodide material featured a much shorter length of 100 nm, correlating well with performance of these materials as solar cells.

[S. D. Stranks et al., Science, 2013]


Carrier lifetime fluctuation- Adapted time-resolved photoluminescence (TRPL) image of CIGS solar cellsOchoa et al. used the coupling of MicroTime 100 and FluoTime 300 to perform photoluminescence as well as time-resolved photoluminescence (TRPL) microscopy on Cu(In,Ga)Se2 solar cells. The charge carrier lifetime is one of the most critical parameters directly affecting VOC. In this study, the effect of inhomogeneities, postdeposition treatments (PDTs) and growth on charge carrier lifetime is investigated.

[Advanced Energy Materials, 2021]


Photoluminescence of perovskite filmsThe application of passivating agents to suppress perovskite defects has been extensively investigated. Zhenwei Ren et al. demonstrates tributylphosphine oxide (TBPO) as a decent passivator for the quasi-2D perovskites achieving high-efficient blue PeLED with external efficiency up to 11.5% and operational stability as long as 41.1 min without any shift of electroluminescence. They used FluoTime 300 to acquire photoluminescence (PL) as well as time-resolved photoluminescence (TRPL) spectra of perovskite films.

[Nano-Micro Letters, 2022]

Upconversion Materials

Measuring and analyzing the fluorescence upconversion using NaYF4:Yb/Er with the FluoTime 300

Upconversion materials have diverse applications including optical thermometry, bio-imaging and solar cells. Upconversion nanoparticles (UCNPs) comprising of rare earth elements are among the most studied materials in this field. FluoTime 300 is employed to measure both steady-state and time-resolved photoluminescence of NaYF4:Yb/Er solution in cyclohexane.
To acquire the long lifetime of NaYF4:Yb/Er, burst mode is applied that is a unique feature of PicoQuant pulsed light sources enabling fast measurements. The analysis reveals a single fluorescence lifetimes of 113 µs.  

Sample courtesy of T. Nyokong, Rhodes University, South Africa

Luminescence spectrum of Er/Yb up-conversion nanoparticles dispersed in cyclohexane

In addition, FluoTime 300 enables the detection of the excitation peak at 980 nm (LDH-D-C-980) that allows the quantum yield measurements. This has been performed on Er/Yb nanoparticles dissolved in cyclohexane.

Sample courtesy of Dr. U. Resch-Genger, BAM

Dyes and Fluorophores

Dynamic anisotropy of Coumarin 6 recorded and analyzed using a FluoTime 300The FluoTime 300 is applied to investigate the dynamic anisotropy of dyes and fluorophores. As an example, the temperature-dependent anisotropy of Coumarin 6 has been studied through automatic measurements of IRF, VV (parallel), VH (perpendicular), and VM (magic angle) polarized decays at various temperature steps via 440 nm excitation. A quick analysis of VV and VH decays clearly shows temperature dependent behavior of the emission anisotropy. The steady-state anisotropy is additionally acquired which were found to be in perfect agreement with the anisotropy values estimated by the Perrin equation.


Photodynamic Therapy

Spectrum and lifetime of singlet oxygen produced by H2TTP in H2O recorded with a FluoTime 300

Singlet oxygen refers to an excited state of molecular oxygen. Due to its reactive properties, singlet oxygen is being used to destroy cancer cells in photodynamic therapy. The detection of this chemical requires high sensitivity in near infrared (NIR) range. FluoTime 300 is applied to measure steady-state and time-resolved fluorescence of singlet oxygen emission produced by H2TTPS in acetone and water. The latter is extremely challenging due to the weak emission. To acquire fast lifetime decay, burst mode is used that is a unique feature of PicoQuant pulsed light sources. A tail fit results in lifetime of 3.4± 0.3 µs, which is in excellent agreement with the published literature value.

Images are used, adapted and reproduced under CC BY licence of each corresponding journals.

Latest 10 publications referencing FluoTime 300

The following list is an extract of 10 recent publications from our bibliography that either bear reference or are releated to this product in some way. Do you miss your publication? If yes, we will be happy to include it in our bibliography. Please send an e-mail to info@picoquant.com containing the appropriate citation. Thank you very much in advance for your kind co-operation.

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