Fluorescence Spectrometers

FluoTime 250

Compact Lifetime Fluorometer

  • Steady-state in emission and time-resolved (TCSPC, MCS) operation mode
  • Easy-handling, compact setup for fast and precise measurements
  • Flexible filter-based emission wavelength selection
  • Fully automated system for lifetimes ranges from ps to s
  • Intuitive acquisition and analysis EasyTau 2 software


  • Micro-Photoluminescence upgrade via confocal microscope coupling
  • Optional UV-Vis monochromator
  • Various sample holders for handling liquid and solid samples with temperature control option

FluoTime 250 - Fully automated lifetime spectrometer with high performance opticsThe FluoTime 250 is a compact table-top though flexible lifetime fluorometer for life science, materials science, and photochemistry applications. This setup enables users to carry out routine lab work quickly, reliably and with high precision. Our fully automated setup contains the complete optics and electronics for recording fluorescence and photoluminescence decays by means of Time-Correlated Single Photon Counting (TCSPC) or Multichannel Scaling (MCS) from a few picoseconds to several seconds. FluoTime 250 is filter-based though it can be equipped with an additional monochromator for UV-Vis spectral range. This fluorescence spectrometer offers high spectral and temporal resolution that allow the acquisition and analysis of

FluoTime 250 is designed to be equipped with PicoQuant picosecond pulsed light sources. All components are being controlled by EasyTau 2 software. A unique feature of PicoQuant spectroscopy software are its three operation modes:

  • Wizard mode provides step by step assistance
  • Customized mode enables full control over the instrumental parameters
  • Scripting mode allows automation of measurement routines.

The latest version of the software, EasyTau 2.3, supports the integration of Prima and Taiko PDL M1.



Recent publications by researchers using the FluoTime 250

Effect of a Phosphorus Additive on Luminescent and Scintillation Properties of Ceramics
Ermakova, et al.
Ceramics 2023
Photoluminescence kinetics of GYAGG-Ce ceramic sample with different relative densities (340 nm excitation)

Ligand-Metal Charge Transfer Induced via Adjustment of Textural Properties Controls the Performance of Single-Atom Catalysts during Photocatalytic Degradation
Liu, et al.
ACS Applied Materials & Interfaces 2021
Transient Photoluminescence of single-atom heterogenous catalysts (375 nm excitation)

Symmetry Breaking Charge Transfer in DNA-Templated Perylene Dimer Aggregates
Duncan, Katelyn M., …, and Ryan D. Pensack
Molecules 2022
Lifetime decay traces obtained for DNA HJ templated perylene monomer, transcerse dimer and adjucent dimer solution (405 nm excitation)

"Red Carbon": A Rediscovered Covalent Crystalline Semiconductor
Odziomek, Mateusz,  …, and Dieter Neher, Thomas D. Kühne, Markus Antonietti, Nieves López Salas
Advanced Materials 2022
red carbon in DMF, red carbon in NMP and Coumarine 153 in EtOH


FluoTime Show Day 2021Virtual show day: Closer look at the FluoTime series

Our first FluoTime Show Day was held on June 17, 2021. During this virtual event, an in-depth introduction on PicoQuant spectroscopy products was provided by our application specialists. Request the recording and learn how our products can help you in your daily research tasks to carry out steady-state and time-resolved luminescence measurements with a micrometer sized positionable observation volume.

Time-resolved Fluorescence CourseCourse on time-resolved fluorescence

PicoQuant annually holds the European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy". The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the life sciences. For details see the course website.

Optical configuration


Mode of operation
  • Steady-state in emission
  • Time-Correlated Single Photon Counting (TCSPC)
  • Multichannel Scaling (MCS)

Down to 10 pM measured with a coumarin sample, with excitation at 440 nm and emission detected at 495 nm

Lifetime range
  • < 10 ps with TCSPC electronics
  • > 5 s with MCS electronics

Depending on light source and detector choice

Excitation sources

255 - 1200 nm picosecond pulsed light sources with repetition rates up to 80 MHz

Optional monochromator
  • Czerny-Turner type
  • Single monochromator
  • 150 mm focal length
  • One exit port
  • 230 - 920 nm: Photomultiplier Tubes (PMTs)
  • 220 - 890 nm : Hybrid-PMTs
  • 950 - 1400 nm: NIR-PMTs
EasyTau 2 software
  • Windows based acquisition and analysis software
  • Lifetime analysis based on numerical reconvolution procedure and up to 5th exponential decay functions
  • Lifetime distributions, anisotropy, global analysis, rigorous error analysis
  • Assisted mode using Wizards for standardized measurements
  • Customized mode with full control of all hardware parameters
  • Scripting mode for automation of routine measurements

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

Sample holders



Temperature stabilized cuvette sample holderTemperature stabilized cuvette (for liquid samples)
1x1cm cuvettes holder for liquid samples that can be connected to an external water bath for temperature stabilization via cable glands at the units front size.

Sample holder for solid samplesFront face (for solid samples e.g. thin films and powder)
Holder designed for measuring small solid samples including coated substrates, thin films, or wafers. The sample position and angle with respect to the excitation beam can be manually adjusted.

Peltier cooled cuvette sample holderPeltier cooled cuvette (for liquid samples)
1x1 cm cuvette holder for liquid samples that features a Peltier element with a temperature range of -15 to 110 °C. The temperature is fully controlled by EasyTau 2 software and can thus be automated to carry out measurements at various temperatures.

Liquid nitrogen cryostat for the FluoTime 250Liquid nitrogen cryostat (for liquid and solid samples)
The temperature range of the cryostat is 77 to 500 K. The sample mounting unit is equipped with an adapter to fit to the sample chamber.

Fluorescence Measurement of Extremely Dilute Samples

Testing the sensitivity of the FluoTime 250 by measuring a dilution series of Coumarin 6FluoTime 250 provides high sensitivity enabling users to determine fluorescence intensity and lifetime decay of extremely dilute samples with concentration as low as a few pM.
As an example, fluorescence emission of a dilution series of Cumarine 6 in ethanol has been measured at 495 nm utilizing FluoTime 250 and 440 nm excitation laser. The plot depicts a nice linear dependency of count rate on dye concentration down to 20 pM which demonstrate the high sensitivity of the setup.

Dyes and Fluorophors

Recording and analyzing the time-resolved emission of ATTO425 in water with the FluoTime 250FluoTime 250 is designed for routine lab works and is an optimal setup for fluorescence lifetime measurements of optical probes such as dyes and fluorophores in solution, as powder or thin film.

As an example, the time-resolved emission of ATTO425 and Ru(bpy)3 aqueous solution are shown. The lifetime decays can be well described with single exponential decay function, as expected. The fit results in 3.944 ± 0.003 ns and 513.85 ± 0.35 ns for ATTO425 and Ru(bpy)3, respectively, which fits well with reported lifetime values for these dyes. 
Recording and analyzing the time-resolved emission of Ru(bpy)3 in water with the FluoTime 250The samples have been excited at 418 nm using a LDH-P-C-420 pulsed laser. To acquire the long lifetime decay of Ru(bpy)3, burst mode is applied that is a unique feature of PicoQuant pulsed light sources enabling fast measurements. The collected data is analyzed in the fitting module of the EasyTau 2 using a reconvolution tail fit.

Photocatalysis and Photoelectrocatalysis

Transient photoluminescence of catalystsThe relation between catalysts texture and their photophysical-photocatalytic properties has been investigated by Liu et al. Employing various methods including transient photoluminescence via FluoTime 250. Time-resolved measurement of single-atom heterogeneous catalysts featuring highly dispersed Ag and Cu on mesoporous graphitic carbon nitride (C3N4) is performed using 365 nm excitation.

[ACS Appl. Mater. Interfaces, 2021]

Transient photoluminescence of S-doped carbon nitrideJerigova et al. applied FluoTime 250 to study sulfur doped carbon nitride (C3N4) as promising material for development of long-range visible catalysts. The transient photoluminescence using 375 nm excitation is acquired from the samples, prepared from various ratio of sulfur-containing precursor, to characterize their physicochemical properties.

[Advanced Science 2023]

Characterization of Lasing and Scintillation materials

Photoluminescence kinetics of various composition of GAGG at room temperature (340 nm excitation)Scintillation materials are being used to detect various ionizing radiation. Korzhik et al. focused on the partial substitution of Gd with Lu ions in the Ce-doped GLAGG crystalline ceramics. They revealed nonequilibrium carriers near various cationic subsystems in the quaternary compound. PicoQuant FluoTime 250 is employed to measure the photoluminescence kinetics using pulsed excitation at 340 and 540 nm. The luminescence was detected at 550 nm with spectral width of 10 nm.

[Crystals 2022]


red carbon in DMF, red carbon in NMP and Coumarine 153 in EtOHRed carbon is a polymeric semiconductor synthesized from carbon suboxide (C3O2)  for potential application in solar cells or photocatalysis. Odziomek et al. Used FluoTime 250 for spectroscopic analysis of red carbon to determine its chemical structure together with elemnetal analysis methods. Three different samples, namely solution, powder and film are characterized resulting in lifetime values of 1.45 ns, 0.09 ns, and 0.07 ns, respectively.

[Advanced Materials 2022]


Images are used, adapted and reproduced under CC By licence of each corresponsing journals.

Latest 10 publications referencing FluoTime 250

The following list is an extract of 10 recent publications from our bibliography that either bear reference or are releated to this product in some way. Do you miss your publication? If yes, we will be happy to include it in our bibliography. Please send an e-mail to info@picoquant.com containing the appropriate citation. Thank you very much in advance for your kind co-operation.

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