Fluorescence Microscopes

MicroTime 100

Compact Upright Photoluminescence Microscope

  • Confocal time-resolved photoluminescence microscope with single photon detection sensitivity
  • Based on time-correlated single photon counting (TCSPC)
  • Lifetime detection range of a few ps to several ms with ps timing resolution
  • Broad excitation wavelengths from 375 – 1060 nm
  • Detection range 400 nm – 1050 nm
  • Intuitive data acquisition and powerful analysis with SymPhotime software


  • Spectrally resolved measurements via photoluminescence spectrometer (FluoTime 300, FluoTime 250) coupling
  • CCD-based spectograph
  • Charge carrier diffusion upgrade
  • Superconducting Nanowire Single-Photon Detector (SNSPD)
  • Heating Stage
  • Cryostat

MicroTime 100 is a compact and flexible confocal microscope. It is an ideal time-resolved photoluminescence imaging setup for various materials science applications including solar cells, light emitting diodes (LEDs), single emitters characterization, and in general semiconductor research. MicroTime 100 is based on a conventional upright microscope body (BX43) that permits easy access to a wide range of sample shapes and sizes. For further flexibility, a BXFM body together with a specialized stand can be employed.

MicroTime 100 -  Upright time-resolved fluorescence microscope

This confocal microscope is designed to be equipped with PicoQuant picosecond pulsed light sources, Time-Correlated Single Photon Counting (TCSPC) electronics and single photon sensitive detectors. This combination enables photoluminescence lifetime detection down to a few ps and up to several ms. All components are being controlled by SymPhoTime 64 software which is a powerful analysis software. Numerous measurements can be carried out using MicroTime 100 such as

MicroTime 100 can be coupled with PicoQuant photoluminescence spectrometers as well as spectrograph enabling multimodal imaging where spectral and temporal information can be collected from the solid samples at high spatial resolution.

Recent publications by researchers using the MicroTime 100

Scientific guidance and user training

Logo of the Time-resolved Microscopy CoursePicoQuant annually holds the European short course on "Time-resolved Microscopy and Correlation Spectroscopy". The course is intended for individuals wishing an in-depth introduction to the principles of time-resolved fluorescence microscopy and its applications to the Life Sciences. This 3-day event consists of lectures as well as instrumentation and software hands-on training.

Detailed specifications are included in the datasheet.

  • Confocal
  • Upright (BX43 body or BXFM body with specialized stand)
  • Objectives selected according to user applications, from simple air to immersion, long-distance, or specialized IR objectives
  • Illumination sources:
    • Transmission illumination unit included in BX43
    • Widefield epi illumination (optional)
    • Camera for top illumination (optional)
    • Gooseneck side-on illumination (optional)
  • Scanners:
    • XYZ piezo objective scanner with 80 x 80 x 80 µm scan range at nominal 0.3 nm resolution positioning accuracy
    • Large area scanning table with 7.5 x 7.5 cm scan range at nominal 0.6 µm positioning accuracy (optional)
Excitation Sources
  • Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz
  • 375 to 1060 nm excitation wavelengths
  • Pulsed, burst, CW operation mode
  • Laser Combining Unit (LCU) combining up to 5 excitation sources
  • Single Photon Avalanche Diodes (SPADs)
  • Hybrid-Photomultiplier Tubes (Hybrid-PMTs)
  • NIR-PMT with > 1400 nm detection range with FluoTime 300 coupling (optional)
  • Exit port for coupling to FluoTime 300 or spectograph (optional)

Up to four parallel detection channels

Data Acquisition

Accessible lifetime ranges from < 30 ps to > 5 ms

Based on Time-Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS) measurement mode

Simultaneous data acquisition of up to four detection channels


Windows-based SymPhoTime 64

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.

Solar Cells and PhotovoltaicsTRPL mapping image of a MAPbI3 polycrystalline thin film and charge carrier diffusion profile of a MAPbI3 grain

Understanding carrier recombination processes in metal halide perovskites is fundamentally important to further improving the efficiency of perovskite solar cells, yet the accurate recombination velocity at grain boundaries (GBs) has not been determined. In this work, Prof. Zhenyi Ni et al. applied MicroTime 100 coupled with FluoTime 300 to map the transient photoluminescence pattern changed induced by nonradiative recombination of carriers at GBs.

[Science Advances 2022]

Also check our webinar with Prof. Jinsong Huang on "Microscopic study of defects in metal halide perovskites"

Light Emitting Diodes (LEDs)

Photoluminescence decay dynamics of ZnCdSe core, ZnCdSe_ZnSe core_shell, TBP-treated ZnCdSe_ZnSe core_shell QDsLEDs technology has a growing impact on future energy consumption. Quantum dots (QDs) are among promising materials for fabrication of next generation LEDs due to their high efficiency and unique spectral tuning ability via the quantum confinement effect. Onal et al. demonstrated high-performance white LEDs using green- and red-emitting QDs that reach over 150 lumens per electrical Watt. Among other characterization methods, they applied MicroTime 100 to detect time-dependent photoluminescence decays. 

[ACS Photonics, 2022]


Synthesis of achiral carbon dots (CD-eda) and chiral carbon dots (CD-cys, CD-glu, CD-phe, CD-try)Luminescent carbon nanoparticles known as carbon dots (CDs) are promising nanomaterials for theranostics due to their ease of fabrication, biocompatibility, attractive optical properties and further chemical functionaliziation. Das et al. synthesized chiral CDs exhibiting high photoluminescence quantum yield reaching 57%. MicroTime 100 is employed to perform time-resolved photoluminescence measurements using 405 nm laser excitation to investigate the effect of chiral precursors, pH and solvent polarity on the CDs lifetimes.

[Light: Science & Applications, 2022]


MicroTime 100 is a powerful tool for localization of nanodiamond vacancy centers through photoluminescence imaging and performing antibunching measurements. The second order correlation function g(2) is measured under continuous wave (CW) as well as pulsed optical excitation using 520 nm lasers with 5 MHz repetition rate. The black line is experimental data and the red line is a fit to the data which results in the lifetime of 41ns under pulsed excitation. A Hanbury Brown and Twiss configuration is employed for data acquisition.Antibunching of nanodiamond vacancy

Images are used, adapted and reproduced under CC BY licence of each corresponding journals.

Latest 10 publications referencing MicroTime 100

The following list is an extract of 10 recent publications from our bibliography that either bear reference or are releated to this product in some way. Do you miss your publication? If yes, we will be happy to include it in our bibliography. Please send an e-mail to info@picoquant.com containing the appropriate citation. Thank you very much in advance for your kind co-operation.

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