Fluorescence Microscopes

MicroTime 100

Compact Upright Photoluminescence Microscope

  • Confocal time-resolved photoluminescence microscope with single photon detection sensitivity
  • Based on time-correlated single photon counting (TCSPC)
  • Lifetime detection range of a few ps to several ms with ps timing resolution
  • Broad excitation wavelengths from 375 to 1060 nm
  • Detection range from 400 nm to 1050 nm
  • Intuitive data acquisition and powerful analysis with SymPhoTime 64 software


  • Spectrally resolved measurements via photoluminescence spectrometer (FluoTime 300, FluoTime 250) coupling or with the new FlexWave add-on
  • CCD-based spectograph
  • Charge carrier diffusion upgrade
  • Superconducting Nanowire Single-Photon Detector (SNSPD)
  • Heating stage
  • Cryostat

MicroTime 100 is a compact and flexible confocal microscope. It is an ideal time-resolved photoluminescence imaging setup for various materials science applications including solar cells, light emitting diodes (LEDs), single emitters characterization, and in general semiconductor research. MicroTime 100 is based on a conventional upright microscope body (BX43) that permits easy access to a wide range of sample shapes and sizes. For further flexibility, a BXFM body together with a specialized stand can be employed.

MicroTime 100 -  Upright time-resolved fluorescence microscope

This confocal microscope is designed to be equipped with PicoQuant picosecond pulsed light sources, Time-Correlated Single Photon Counting (TCSPC) electronics and single photon sensitive detectors. This combination enables photoluminescence lifetime detection down to a few ps and up to several ms. All components are being controlled by SymPhoTime 64 software which is a powerful analysis software. Numerous measurements can be carried out using MicroTime 100 such as

MicroTime 100 can be coupled with PicoQuant photoluminescence spectrometers as well as spectrograph enabling multimodal imaging where spectral and temporal information can be collected from the solid samples at high spatial resolution.

Recent publications by researchers using the MicroTime 100

Scientific guidance and user training

Logo of the Time-resolved Microscopy CoursePicoQuant annually holds the European short course on "Time-resolved Microscopy and Correlation Spectroscopy". The course is intended for individuals wishing an in-depth introduction to the principles of time-resolved fluorescence microscopy and its applications to the Life Sciences. This 3-day event consists of lectures as well as instrumentation and software hands-on training.

Detailed specifications are included in the datasheet.

  • Confocal
  • Upright (BX43 body or BXFM body with specialized stand)
  • Objectives selected according to user applications, from simple air to immersion, long-distance, or specialized IR objectives
  • Illumination sources:
    • Transmission illumination unit included in BX43
    • Widefield epi illumination (optional)
    • Camera for top illumination (optional)
    • Gooseneck side-on illumination (optional)
  • Scanners:
    • XYZ piezo objective scanner with 80 x 80 x 80 µm scan range at nominal 0.3 nm resolution positioning accuracy
    • Large area scanning table with 7.5 x 7.5 cm scan range at nominal 0.6 µm positioning accuracy (optional)
Excitation Sources
  • Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz
  • 375 to 1060 nm excitation wavelengths
  • Pulsed, burst, CW operation mode
  • Laser Combining Unit (LCU) combining up to 5 excitation sources
  • Single Photon Avalanche Diodes (SPADs)
  • Hybrid-Photomultiplier Tubes (Hybrid-PMTs)
  • NIR-PMT with > 1400 nm detection range with FluoTime 300 coupling (optional)
  • Exit port for coupling to FluoTime 300 or spectograph (optional)

Up to four parallel detection channels

Data Acquisition

Accessible lifetime ranges from < 30 ps to > 5 ms

Based on Time-Correlated Single Photon Counting (TCSPC) and Multi-Channel Scaling (MCS) measurement mode

Simultaneous data acquisition of up to four detection channels


SymPhoTime 64

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.


FlexWave wavelength selection unit for confocal microscopesAdd-on: FlexWave Wavelength Selection Unit for Confocal Microscopes

Feature highlights:

  • Spectral scanning with > 80 % transmission from 400 to 1000 nm for highest sensitivity
  • High temporal resolution without pulse broadening by an optical diffraction grating

Suitable for various applications:

Detailed specifications are included in the datasheet.


  • Spectral range: 400 - 1000 nm
  • Transmission: > 80 % over the whole spectral range
  • Bandwidth min.: 1 nm
  • Bandwidth max.: 80 nm
  • Step width min: 0.1 nm
  • Detection: single detector directly mounted or fiber-coupled multi-channel detection unit
  • Detector types: PMA Hybrid series or SPADs
  • Software: SymPhoTime 64

FlexWave analysis spectrum

Latest 10 publications referencing MicroTime 100

The following list is an extract of 10 recent publications from our bibliography that either bear reference or are releated to this product in some way. Do you miss your publication? If yes, we will be happy to include it in our bibliography. Please send an e-mail to info@picoquant.com containing the appropriate citation. Thank you very much in advance for your kind co-operation.

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