When Lifetime Information Is Not Enough
Time-correlated single photon counting delivers precise fluorescence decay data. Spectral measurements, on the other hand, reveal emission characteristics that distinguish molecular states or environments.
In many laboratory setups, both types of information are available, but rarely at the same time and from the same photons. For experiments at the single-molecule level, this separation can limit interpretation.
A Spectrograph Add-on for the MicroTime 200
The Technical Note describes how a fiber-coupled spectrograph can be connected to the MicroTime 200 in order to access spectral information alongside TCSPC-based lifetime detection.
The document explains:
- how part of the fluorescence signal is directed to an external spectrograph
- how efficient fiber coupling is realized
- which detector configuration enables sensitive spectral acquisition
- how simultaneous lifetime and spectral data were experimentally demonstrated
A proof-of-principle experiment on immobilized single molecules illustrates the achievable sensitivity and correlation of parameters.
Expanding the Experimental View
Observing intensity fluctuations, decay kinetics, and emission spectra within one configuration enables direct comparison of dynamic and spectral behavior. This can be relevant for studies of molecular heterogeneity, spectral switching, or nanoscale emitters.
The Technical Note provides the optical layout, detector integration details, and experimental data required to assess whether this approach fits your application.
Download the full Technical Note for a detailed description of the setup and validation measurements.
