MicroTime 200 STED Add-On

Super-resolution upgrade for time-resolved confocal imaging

Open super-resolution STED add-on for MicroTime 200 that enables nanoscale structural imaging with time-resolved contrast.
MicroTime 200 STED add-on for super-resolution confocal imaging

Key-Benefits

Resolve nanoscale structures below 50 nm
Synergies with FLIM and FCS
Robust implementation with minimal setup effort
Open and flexible experimental design
Expert-level application support

Integrating STED into MicroTime 200

Comparison of confocal and time-gated STED (gSTED) imaging of tubulin acquired with the MicroTime 200 STED system, demonstrating enhanced filament resolution and improved structural contrast beyond the diffraction limit.

Super-Resolution Without Compromising Flexibility

The STED Add-on expands MicroTime 200’s open, time-resolved microscopy platform with robust super-resolution capabilities. Building on its single-molecule sensitivity, flexible optics, and powerful SymPhoTime 64 workflow, it enables sharper structural insights and richer analytical depth, while preserving the system’s hallmark adaptability to advanced methods and custom configurations.

STED Capabilities at a Glance

  • Super-resolution below 50 nm for resolving nanoscale structures previously indistinguishable in confocal images.
  • Triple-species STED imaging using fluorescence pattern matching for accurate multicolor co-localization.
  • Reduced observation volumes for STED-FCS, enabling diffusion measurements at higher concentrations and in heterogeneous environments.
  • gSTED time-gating to isolate late photons, further shrinking the effective detection volume for improved resolution and sensitivity.
  • Stable, donut-shaped depletion beam generated by the EASYDOnut phase plate without the need for complex alignment.
  • Full compatibility with MicroTime 200, maintaining access to advanced methods, custom configurations, and future system extensions without restricting the open design.
  • Full STED workflow integration in SymPhoTime 64, with time-gating, gSTED, and multi-species pattern matching.

 

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Datasheet MicroTime 200

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