August 16, 2025

SHG Imaging with Picosecond Pulsed Lasers

Exploring an Alternative to Femtosecond Sources

Second-harmonic generation (SHG) imaging is widely used to study non-centrosymmetric materials. A recent poster explores how picosecond pulsed lasers can be used for SHG microscopy while simplifying experimental setups.
Second-harmonic generation image of MoS2 monolayer with intensity scale

SHG Imaging and the Role of Laser Sources

Second-harmonic generation (SHG) imaging is widely used to investigate non-centrosymmetric materials such as 2D semiconductors, lithium niobate, and biological structures. The technique provides insights into crystal orientation, layer stacking, grain boundaries, and mechanical strain.

In many laboratories, SHG imaging is almost automatically associated with femtosecond laser sources. While powerful, these systems increase experimental complexity and introduce stricter laser safety requirements.

Picosecond Lasers as an Alternative Approach

The poster demonstrates that high-power picosecond pulsed lasers can be used as an alternative excitation source for SHG imaging. Using a confocal microscope setup, SHG signals excited at 1064 nm were recorded together with time-resolved photoluminescence (TRPL), allowing multiple optical contrasts to be measured from the same region of a sample.

Experiments on monolayer MoS₂ and WSe₂ illustrate how reflection images, SHG signals, and lifetime data can be collected within a single microscope configuration.

Second-harmonic generation image of MoS2 monolayer with intensity scale
Second-harmonic generation intensity image of a MoS₂ monolayer acquired with 1064 nm excitation. The photon-counting scale illustrates SHG signal strength, while the absence of measurable fluorescence lifetime confirms the instantaneous and coherent nature of the SHG process.

Download the Poster

The full poster describes the experimental setup, measurement parameters, and example datasets for SHG and TRPL imaging of 2D materials.

Poster: SHG Imaging Microscopy

Second-harmonic generation imaging with picosecond lasers reveals crystal structure, defects, and layer orientation in advanced materials.

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Galaan Merga

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Galaan Merga

Scientific Writer, PicoQuant

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