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MicroTime 200

MicroTime 200

Modular Confocal Microscope with Single Molecule Sensitivity

Modular, customizable, time-resolved confocal microscope with single-molecule sensitivity for life and materials science.
MicroTime 200 time-resolved confocal microscope system on optical table
Table of contents

Key Benefits

Single-molecule sensitivity and precision
Flexible experimental design
Customizable and open workflows
Long-term reliability and upgradeability
Sustainable system design
Expert-level application support

Redefining Openness and Flexibility in Confocal Microscopy

The MicroTime 200’s modular design allows flexible excitation and detection from deep UV to IR, with open architecture for customization and transparent data analysis.

Designed Around Your Research Questions to Enable New Experimental Designs

Rather than being a “black box”, MicroTime 200 gives researchers the freedom and transparency to define their own approach. Its open architecture evolves with experimental needs, ensuring consistent performance, reliable results, and lasting value in cutting-edge research.

A MicroTime 200 confocal microscope in active use at the laboratory of Prof. Sílvia Brito Costa and Pedro Paulo, demonstrating how upgradeable system design enables high-quality research over more than two decades.

Proven Reliability for Long-Term Research

The most effective measure for increased sustainability is the increase lifetime of the purchased device.

  • Built to last: robust opto-mechanics, stable alignment, long component lifecycles.
  • Built it as your needs grow: add channels, detectors, or lasers as your research evolves.
  • Stay always state of the art: extend system lifetime with modular swaps instead of full replacements.
  • Expert partnership: scientific support, training, and method onboarding throughout the system’s life.
MicroTime 200 STED Add-On: Super-resolution upgrade for time-resolved confocal imaging.

Open Super-Resolution Imaging with STED

The MicroTime 200 STED add-on enables time-resolved super-resolution imaging with spatial resolution below 50 nm. A STED module fitting to the open design of MicroTime 200 designed for robust, alignment-free operation. It combines the easySTED concept with fast galvo scanning to reduce phototoxicity and bleaching. Time-gated detection improves contrast and background suppression, extending super-resolution imaging to demanding fluorescence lifetime and FCS studies.

NovaFLIM: Fluorescence Lifetime Imaging Analysis Software

Analyze. Visualize. Understand.

SymPhoTime 64 provides full control over data acquisition and standard analysis workflows for time-resolved microscopy. For streamlined FLIM, FLIM-FRET and anisotropy analysis needs data can be further loaded to, NovaFLIM  which delivers accelerated analysis, modern visualization, and powerful tools for extracting quantitative insight from complex datasets.This powerful and modular software combination addresses demanding applications while it offers transparent data handling, open data formats and extended metadata.

Relevant for Your Research​

Matching Applications & Methods​

All Applications & Methods
Switcher A&M
Illustration of protein conformational dynamics
Life Science

Dynamic Structural Biology

Time-resolved photoluminescence emission spectrum showing three peaks from different semiconductor layers, illustrating layer-specific recombination dynamics.
Materials Science

Semiconductor Research

Schematic illustration of nanostructured materials on a substrate highlighting heterogeneous nanoscale architectures studied by optical and time-resolved characterization.
Materials Science

Nanomaterials Research

Trusted by Researchers Worldwide

What Researchers Say

The MicroTime 200 has made complex technology usable for every lab. We have been able to publish our first paper within a few months. In the last nearly 10 years the performance of this machine is outstanding.
Professor Kankan Bhattacharyya
Indian Institute of Science Education and Research Bhopal (IISERB)
First I would like to thank you for the quality of the MicroTime 200 FLIM microscope and software that provides amazing possibilities.
Dr. Géraud Delport
Institut Photovoltaïque d'Ile-de-France (IPVF)
We have an extensive usage of our MicroTime 200, I would say almost 24/7.
Professor Andrea Soranno
Department of Biochemistry and Molecular Biophysics, Washington University (WashU) in St. Louis
I’m very glad to have the opportunity to work with PicoQuant instrument and to have the support of a strong experienced team in time-resolved fluorescence to explore new horizons.
Arnaud Spangenberger
Centre national de la recherche scientifique
Scientific Insights & Resources

Latest Blog Articles

Side-by-side AFM topography and fluorescence lifetime image of 100 nm fluorescent beads on glass, showing structural features in AFM and selective fluorescence contrast in FLIM.
March 11, 2011

Combining AFM with Time-Resolved Confocal Microscopy

Modular MicroTime 200 configuration illustrating flexible excitation and detection modules
March 12, 2023

MicroTime 200 Customization: Modular Confocal Microscopy

The MicroTime 200 confocal microscope at the University of Lisbon, demonstrating long-term research continuity since its installation in 2002.
November 12, 2023

When Longevity Becomes a Scientific Advantage

Side-by-side AFM topography and fluorescence lifetime image of 100 nm fluorescent beads on glass, showing structural features in AFM and selective fluorescence contrast in FLIM.
March 11, 2011

Combining AFM with Time-Resolved Confocal Microscopy

Modular MicroTime 200 configuration illustrating flexible excitation and detection modules
March 12, 2023

MicroTime 200 Customization: Modular Confocal Microscopy

The MicroTime 200 confocal microscope at the University of Lisbon, demonstrating long-term research continuity since its installation in 2002.
November 12, 2023

When Longevity Becomes a Scientific Advantage

Visit our science hub

A Modular Platform Built Around Your Experiment

MicroTime 200 is designed as a flexible, modular system that adapts to a wide range of experimental requirements. Excitation, detection, and scanning can be configured independently, allowing researchers to optimize every part of the measurement chain for their specific application.

PicoQuant pulsed laser and LED sources

A Flexible Excitation Subsystem

Select from a broad range of laser sources covering 255–900 nm to match fluorophores, quantum emitters, or fluorescent proteins. Create complex excitation patterns like pulsed-interleaved excitation (PIE) for FRET or multi-wavelength sequences for contrast without redesigning your setup.

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PicoQuant's hybrid and SPAD single-photon detectors

Detection with Single Photon Sensitivity

Mix and match up to six detection channels for maximum flexibility. Combine high-timing-precision hybrid detectors with high-QE SPADs to balance count rate and spectral coverage. Minimal afterpulsing and short dead time maintain signal integrity during fast decays, antibunching measurements, and correlation analyses in both biological and solid-state samples.

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Overview image of PicoQuant Time Tagging and TCSPC units including HydraHarp 500, MultiHarp 150/160, PicoHarp 330, and TimeHarp 260.

Time Tagging and TCSPC Electronics

Capture every photon with picosecond timing precision and high throughput. Advanced time tagging and TCSPC electronics enable accurate correlation, lifetime, and timing analysis across a wide dynamic range. Short dead times and scalable channel configurations ensure reliable measurements for both single-molecule studies and complex photonic experiments.

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MicroTime 200 integrated with AFM for correlated FLIM measurements

Customized Setups for Demanding Experiments

MicroTime 200 can be customized to match highly specific experimental demands, from correlated AFM–FLIM measurements and cryogenic single-molecule studies to spectrally resolved and multiphoton experiments. 

Explore real-world customization examples in our blog article.

In-Depth Scientific Resources

Premium Resources

Access in-depth application notes and scientific posters with detailed methods, measurement data, and real-world use cases.

Application Note: Time-Gated FCS

Time-gated FCS for improved background suppression and accurate concentration measurements

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Application Note: FRET Analysis Using PIE

FRET analysis using Pulsed Interleaved Excitation (PIE) with the MicroTime 200 for accurate single-molecule studies and improved FRET efficiency determination

Download

Information Bundle: FCS and Related Techniques

Information bundle on fluorescence correlation spectroscopy (FCS) covering quantitative FCS, liFCS, dual-focus FCS, FLCS, calibration methods, and diffusion analysis.

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Customer Video: Probing the Dynamics and Interaction Mechanisms of IDPs with Single-Molecule Spectroscopy

In this customer video, Ben Schuler (University of Zürich) shows how single-molecule FRET combined with correlation spectroscopy and microfluidic mixing reveals the dynamics of intrinsically disordered proteins across timescales and environments.

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Customer Video: Multimodal Microscopy of Halide Perovskite Solar Cells

In this video, Sam Stranks (University of Cambridge), gives an overview of halide perovskite solar cell research using multimodal microscopy to study luminescence, recombination, degradation, and pathways to improve efficiency and stability.

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Expandable Through Powerful Add-Ons

A range of standard add-ons extends MicroTime 200 to support diverse experimental requirements. Many of these options can be combined, allowing researchers to build powerful, application-specific configurations tailored to their lab’s needs.

Correlated FLIM, STED, and AFM nanoscale imaging data

AFM Integration for Correlated Nanoscale Insight

Combine single-molecule fluorescence with nanometer-resolved AFM measurements for structural and dynamic correlation.

FlexLambda wavelength selection unit for spectrally resolved TRPL microscopy

Flexible Spectral Selection with FlexLambda

FlexLambda extends MicroTime 200 with fast, tunable wavelength selection for spectrally resolved TRPL experiments. It enables point spectroscopy, wavelength-dependent lifetime imaging, and TRES measurements without compromising temporal resolution.

Two-photon FLIM image of tissue with lifetime contrast

Two-Photon Excitation for Deep and Robust Imaging

Enable deep-tissue imaging and time-resolved photoluminescence with a fully enclosed, user-alignable TPE module.

Technical Documentation and Data

Technical Downloads

Datasheet MicroTime 200

MicroTime 200 datasheet provides detailed technical information

Technical Note: AFM with MicroTime 200

Combining atomic force microscopy with confocal microscopy

Technical Note: Spectrograph Add-On MicroTime 200

Resolve time and spectral information simultaneously

Technical Note: 2P-FLIM in Living Cells

Using two-photon FLIM for ion sensing in living cells

Expand Your System

Complete your System

Combine compatible components to build a complete system tailored to your experimental requirements and measurement workflows.

NovaFLIM software interface for fluorescence lifetime imaging analysis
Fluorescence Lifetime Imaging Analysis Software
Fast GPU-accelerated analysis of FLIM, FLIM-FRET, and anisotropy data
FLIM analysis modes: Fast Lifetime, decay fitting, phasor plots, and pattern matching
Efficient batch analysis of z-stacks, time-lapse series, and stitched images
Advanced, flexible ROI handling and data export options
Reproducible ROI selection using histograms of fitted parameters
Get a quote
MicroTime 200 STED add-on for super-resolution confocal imaging
Super-resolution upgrade for time-resolved confocal imaging
Resolve nanoscale structures below 50 nm
Synergies with FLIM and FCS
Robust implementation with minimal setup effort
Open and flexible experimental design
Expert-level application support
Get a quote
Learn, Connect, and Exchange

Meet PicoQuant at Scientific Events

Juni, 16–19, 2026
Coimbra, Portugal
Booth: 36

ELMI 2026

During ELMI, attendees can experience Luminosa, our powerful Single Photon Counting Confocal Microscope for fluorescence lifetime imaging, firsthand. Through a series of on-site workshops, attendees will have the opportunity to explore its capabilities in a practical setting. We will also highlight the latest features in Luminosa, including LumiPy, enabling flexible data analysis and integration into custom workflows. Join us to discover how our solutions support cutting-edge microscopy and imaging research.

May 19-22, 2026
Heidelberg, Germany

EMBL Symposium: "Cellular Mechanisms driven by Phase Separation"

Get new insights into cellular dynamics with Luminosa and SPAD-based FCS

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