Zero-mode waveguides (ZMWs) reduce the effective observation volume, enabling FCS measurements at micromolar fluorophore concentrations while maintaining low molecular occupancy.
Our latest article shows how Luminosa combines ZMW imaging and positioning with quantitative FCS. It also explores programmable multi-ZMW measurements with the LumiPy Python interface, demonstrated in a proof-of-concept experiment across 88 ZMWs.
For more details on the measurements and results, download our new Application Note “FCS in Zero-Mode Waveguides with Luminosa” is now available for download.
Read the article in our Science Hub and download the Application Note >
New to Zero-Mode Waveguides?
Learn how ZMWs enable single-molecule fluorescence measurements at higher concentrations in our article about the principles of ZMW.