Liquid-liquid phase separation (LLPS) is a physical phenomenon where a liquid containing several components demixes into two separate phases – a dense, condensed and a dilute phase – depending on the concentration and other stimuli. One key method to study LLPS is fluorescence microscopy, which allows researchers to visualize the formation and dynamics of condensates, and to observe the behavior of molecules inside condensates in real-time.
In our new application note our application specialist Maria Loidolt-Krüger describes how FLIM and multi-point FCS shed light on the behavior of synapsin-1 in biological condensates.