These technical notes cover applications or technical aspects of our time-resolved spectrometers (FluoTime Series). This includes, for example, anisotropy and sensitivity measurements using the FluoTime spectrometers as well as an example of time-resolved spectroscopy of proteins using the FluoTime 100.
Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume with the FluoMic add-on
In this white paper, we show how the combination of a FluoTime 300 with the FluoMic add-on becomes a powerful tool for collecting spectral and temporal information from a sample using a micrometer-sized, positionable observation volume. The capabilities of such an instrument are demonstrated through a series of examples reflecting a broad range of application scenarios.
Performing high speed lifetime measurements of proteins using a 280 nm picosecond laser
This application note compares measuring steady state and time-resolved fluorescence of Human Serum Albumin (HSA) using a FluoTime 300 spectrometer using either a 280 nm pulsed LED (PLS-280) or a picosecond pulsed UV laser (VisUV-280). The reported results show that using the laser allows for much shorter acquisition times. Furthermore, the narrower temporal pulse width of the laser allows determining short lifetimes with higher accuracy.
Time-resolved Spectroscopy of Proteins Using the FluoTime 100
The application note demonstrates the potential of the FluoTime 100 for determining the dynamics of a single Tryptophane residue in a protein using lifetime and anisotropy measurements
Anisotropy Measurements Using the FluoTime 100
The technical note demonstrates the ease and simplicity of time-resolved anisotropy measurements using the FluoTime 100 fluorescence lifetime spectrometer. It provides a brief explanation of the underlying principle, basic definitions and relations using experimental data as examples. Two data analysis techniques are presented that can easily be adopted for own measurements.
Sensitivity of the FluoTime Series Lifetime Spectrometers
The technical note describes a benchmark test of the two lifetime spectrometers FluoTime 200 and FluoTime 100. Various aspects of fluorescence lifetime measurement of low concentration samples are discussed. The spectrometer sensitivity was evaluated by comparing test results obtained with both spectrometer types using the same sample, excitation source and experimental procedure.