These technical notes cover applications or technical aspects of our time-resolved spectrometers (FluoTime Series). This includes, for example, anisotropy and sensitivity measurements using the FluoTime spectrometers as well as an example of perfomring high speed lifetime measurements of proteins using the FluoTime 300.
Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume with the FluoMic add-on
In this white paper, we show how the combination of a FluoTime 300 with the FluoMic add-on becomes a powerful tool for collecting spectral and temporal information from a sample using a micrometer-sized, positionable observation volume. The capabilities of such an instrument are demonstrated through a series of examples reflecting a broad range of application scenarios.
Performing high speed lifetime measurements of proteins using a 280 nm picosecond laser
This application note compares measuring steady state and time-resolved fluorescence of Human Serum Albumin (HSA) using a FluoTime 300 spectrometer using either a 280 nm pulsed LED (PLS-280) or a picosecond pulsed UV laser (VisUV-280). The reported results show that using the laser allows for much shorter acquisition times. Furthermore, the narrower temporal pulse width of the laser allows determining short lifetimes with higher accuracy.