Combining FLIM-FRET and STED microscopy in vivo using a newly developed FRET pair
Congratulations to Erika Günther (former Postdoctoral Researcher at Max Planck Institute of Colloids and Interfaces, Potsdam, Germany) and her colleagues on their exciting work combining FLIM-FRET and STED microscopy in vivo using a newly developed FRET pair! This combination enables the study of protein interactions in sub-diffraction structures. The group uses FLIM-FRET to monitor the connection of the bacterial filament MamK to the magnetosome membrane via the linker MamJ, and STED to check the filament for fragmentation. Also impressive, they use PicoQuant's 6th MicroTime 200 microscope, built in 2004, for performing this cutting-edge research. This system features a custom-built STED add-on using one of the first P-FA-765 lasers from PicoQuant for depletion. If you want to build on their research, good news: by now, an alignment-free, multicolor easySTED add-on is available for our MicroTime 200 confocals to perform STED-FLIM-FRET and STED-F(LC)CS experiments.
Article at nature.com
PicoQuant's MicroTime 200 STED