Time-Resolved Spectroscopy for Bioanalytical Applications: Advanced Time-Domain Fluorescence Spectroscopy and Microscopy (FLIM, FRET, FCS & SMD)

Summary

The workshop was dedicated to interested students and scientists from Life Sciences having a certain background in fluorescence and confocal microscopy and looking to extend their knowledge to the benefits of time-resolved methods. In the afternoon we provided the opportunity to see state-of-the-art instruments working and gave a detailed explanation of key features by highly recognized instructors.

Product demos

Product demos that where offered included:

• MicroTime 200: time-resolved confocal microscope with single molecule sensitivity
• FluoTime 200: modular fluorescence lifetime spectrometer
• SPCE-Setup: Surface Plasmons Assisted Microscopy (SPAM) combines time-resolved microscopy with nanotechnologies, utilizing surface plasmon resonance (SPR) excitation and surface plasmons-coupled emission (SPCE).

Lecturers / Instructors

• Julian Borejdo, Professor in the Department of Molecular Biology and Immunology in UNTHSC.
• Ignacy Gryczynski, Professor and Director of Microscopy Core Facility in the Department of Cell Biology and Genetics in UNTHSC.
• Zygmunt (Karol) Gryczynski, Professor in the Department of Molecular Biology and Immunology in UNTHSC.
• Samantha Fore, Post-doc at the Center for Biophotonics, UC Davis, Sacramento.
• Thomas Dertinger, Post-doc at the group of Shimon Weiss, UCLA.
• Peter Kapusta, Senior Scientist at PicoQuant.
• Uwe Ortmann, Head of Sales and Marketing at PicoQuant.

Time schedule

Time	Speaker	Title of lecture / talk
8:30	Karol Gryczynski / Uwe Ortmann	Welcome & short introduction of UNTHSC / PQ
8:45	Peter Kapusta	The FluoTime Series: a versatile family of TCSPC based fluorescence spectrometer
9:30	Karol & Ignacy Gryczynski	FluoTime: time-resolved measurements, fluorescence lifetime standards, limits of resolution
10:00	Coffee break
10:20	Uwe Ortmann	The MicroTime Series: a versatile family of TCSPC based confocal fluorescence microscopes with single molecule sensitivity for FCS, FRET & FLIM
10:50	Samantha Fore	Time-resolved fluorescence spectroscopy applications in biology using the MicroTime 200 - from single molecules to living organisms
11:30	Thomas Dertinger	Two focus FCS: a new tool for ultraprecise measurements
12:10	Karol Gryczynski / Julian Borejdo	Single molecule conformational dynamics using Surface Plasmons Assisted Microscopy (SPAM)
12:50	Lunch (at the Museum - for all participants)
14:30	Product demo 1	FluoTime 200, MicroTime 200 and SPCE setup
15:20	Product demo 2	FluoTime 200, MicroTime 200 and SPCE setup
16:10	Coffee break
16:40	Product demo 3	FluoTime 200, MicroTime 200 and SPCE setup
18:00	Beer & Pizza reception (Room 434 - for participants of product demonstrations only)

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

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