PicoQuant’s 8th Time-resolved Microscopy Course introduced novel FLIM method

From March 8 to 10, 2016, industry leading microscopy companies joined PicoQuant's 8th annual course on Time-resolved Microscopy. Instructors from Leica, Nikon, Olympus, and Zeiss came to Berlin to provide practical training sessions on their confocal microscopes. During these sessions, PicoQuant introduced a novel fluorescence lifetime imaging (FLIM) method named rapidFLIM that allows recording quantitative lifetime data at very high count rates thanks to optimized electronics with extremely short dead times.

The practical sessions were accompanied by lectures from renowned scientists, covering both the basics and advanced topics of time-resolved fluorescence microscopy. This years lecturer panel included Guiseppe Vicidomini from the Italian Institute of Technology, Chris Dunsby from the Imperial College London, Jörg Enderlein and Fred Wouters from the University of Göttingen, Mark Hink from the University of Amsterdam, and Andreas Bülter from PicoQuant.

The course provided an in-depth introduction to principles and applications of time-resolved fluorescence microscopy from a theoretical and experimental point of view. The hands-on sessions covered topics such as instrumentation and data analysis techniques, correlation methods (FCS, FCCS, FLCS), imaging methods including FLIM, FRET and FLIM-FRET, single molecule as well as STED super-resolution microscopy. The experiments were conducted on time-resolved confocal microscopes from PicoQuant as well as laser scannnig microscopes from Nikon, Leica, Olympus, and Zeiss, each upgraded for time-resolved data acquisition through dedicated kits from PicoQuant.

The course program including practical session organized in collaboration with the market-leading companies was highly valued by the participants. Thanks to the positive feedback and high demand, PicoQuant will continue to organize this course and the next installment will take place in Spring 2017.

Downloads

• Press release 8th microscopy course 2016 (zip, 1.05 MB )