Time-Resolved Microscopy Course

Scope

Our course provides an in-depth introduction to the world of time-resolved fluorescence microscopy with a focus on life science applications. It will be held as a fully online event, including theoretical as well as practical sessions, discussions and networking in an interactive format. The course combines a series of lectures by experts in the field with practical sessions on systems from the market-leading companies Nikon, Olympus, Zeiss, and PicoQuant.

Who should attend?

The content is geared towards both novice researchers as well as those having some previous experience with time-resolved methods and applications.

Why should you attend?

Join our event to:

Learn the principles behind time-resolved fluorescence microscopy
Get to know powerful applications that will help advance your research
Understand how time-resolved data is acquired and analyzed
Interact directly with experts to discuss your research and foster collaborations
See confocal microscopes from various companies live in action
Talk to and socialize with your fellow scientists

And all of this from the safety and comfort of your home.

Which topics are covered?

Attending the lectures and practical sessions will provide you with a better understanding of the following topics:

Instrumentation and data analysis
Correlation spectroscopy methods: FCS, FCCS, FLCS, ...
Imaging methods: FLIM, rapidFLIM, FRET, FLIM-FRET, ...
Single molecule microscopy

Practical sessions

Confocal microscopes from Nikon, Olympus, Zeiss, and PicoQuant will be used during the practical sessions. On each day we will perform a live demonstration on one of the systems that focuses on a special topic (see schedule). This will give everyone a chance to familiarize themselves with all featured instruments and techniques and ask live questions during these sessions!

Contact

Course coordinator: Marta Kolonko

Tel: +49-30-1208820-87
Fax: +49-30-128820-90
Email: trfcourse@picoquant.com

Please note: schedule and content of this event are subject to change without notice.

Course instructors

Alessandro Esposito
University of Cambridge, United Kingdom
Michael Ebner
Leibniz Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany
Jelle Hendrix
Biomedical Research Institute, Hasselt, Belgium

Stefanie Weidtkamp-Peters
Heinrich-Heine-Universität Düsseldorf, Germany
Olaf Schulz
PicoQuant GmbH, Berlin, Germany
Samaneh Rezvani
PicoQuant GmbH, Berlin, Germany

Schedule (as of January 1, 2021. Subject to changes)

The schedule can also be downloaded here (PDF)

Note: All times given here refer to the local time in Berlin (UTC+1)

Tuesday, March 2	Wednesday, March 3	Thursday, March 4	Friday, March 5
9:45 Opening remarks and technical warm-up	9:50 Welcome	9:50 Welcome	9:50 Welcome
10:00 Introduction to FLIM Olaf Schulz PicoQuant, Berlin, Germany	10:00 Introduction to FCS Olaf Schulz PicoQuant, Berlin, Germany	10:00 Raster and temporal ICS: principles, novel developments and applications Jelle Hendrix Biomedical Research Institute, Hasselt, Belgium	10:00 Fast multiplexed biochemical imaging Alessandro Esposito University of Cambridge, UK
10:45 Q&A session with speaker	10:45 Q&A session with speaker	10:45 Q&A session with speaker	10:45 Q&A session with speaker
11:00 Break	11:00 Break	11:00 Break	11:00 Break
11:30 Introduction to FLIM/FRET Samaneh Rezvani PicoQuant, Berlin, Germany	11:30 Introduction to FLCS, Single Molecule Microscopy Samaneh Rezvani / Olaf Schulz PicoQuant, Berlin, Germany	11:30 Harnessing the power of F(C)CS to address protein-membrane interactions in living cells Michael Ebner Leibniz Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany	11:30 Investigating protein-protein interaction in plant tissue using FLIM-FRET Stefanie Weidtkamp-Peters Heinrich-Heine-Universität Düsseldorf, Germany
12:15 Q&A session with speaker	12:15 Q&A session with speakers	12:15 Q&A session with speaker	12:15 Q&A session with speaker
12:30 Break	12:30 Break	12:30 Break	12:30 Break
14:00 Practical session: FLIM basics Nikon	14:00 Practical session: FCS/SMD Basics MicroTime 200 PicoQuant	14:00 Practical session: Advanced FCS Zeiss	14:00 Practical session: Advanced FLIM/FRET Olympus
16:00 Open discussion	16:00 Open discussion	16:00 Open discussion	16:00 Open discussion
16:30 End of day 1	16:30 End of day 2	16:30 End of day 3	16:30 End of course

Course fees

	until December 31, 2020	January 1, 2021 until January 31, 2021
Academic/University	150 €	200 €
Industry and Private Sector	300 €	400 €

Please note the terms and conditions.

For payment you can choose between credit card (Visa, Master Card) and bank transfer. Possible bank charges have to be paid by the participant. Please note that we do not accept checks.
After online registration, you will receive an email notification including a PDF file that includes information on the payment procedure.
All payments have to be received within 14 days after date of registration.
We will send an email confirming your participation once we have received your payment. If payment is overdue, your registration will not be processed and considered invalid.
A receipt of payment will be included in our email confirmation of participation.
Cancellation of registration must be submitted in writing or via email and is valid only with acknowledgment of receipt by PicoQuant GmbH. A refund of registration fees is dependent on the notice given:
- For cancellations made until January 31, 2021, 75 % of the received registration fee will be reimbursed. In case of cancellations after January 31, 2021, 25 % of the registration fee will be reimbursed.
- It is possible to name and send a substitute participant..
Schedule and content of this event is subject to change without notice.
PicoQuant records photographs and video material (including audio) of participants at the events. By registering for a PicoQuant event, you agree that we may include images of yourself (either as a full image or in parts) for promotional purposes in various publications (press releases, promotional activities, event website, etc.). This material may also appear on our internet web page or on related social media pages. If you do not want us to use pictures or video material in which you appear, please inform us in written form.