2nd Southeast Asian Course on "F-techniques: FCS, FCCS, FLCS, FRET, FLIM and FRAP"

Aims and purpose

The course was intended for individuals wishing an in-depth introduction to the F-techniques and their application to the life sciences. Participants where normally graduate students or postdocs who are using fluorescence microscopy in their research. Most participants had some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, got the opportunity to enter this exciting field in a very effective way and benefited especially from the experimental section.

Course organisation

The course consisted of lectures and practical sessions.

The lectures on Monday and Tuesday (July 16/17) dealt with fluorescence, theory of F-techniques (FRET, FLIM, FCS and FRAP), FCS variants, instrumentation and data analysis. The major FRET methods where be covered in detail. The lectures on Wednesday and Thursday (July 18/19) covered research applications of F-techniques in cell and developmental biology. On Friday (July 20) lectures focused on superresolution microscopy, PALM, STORM and structured illumination.

Practical sessions (afternoons of July 17-20) covered steady-state and time-resolved experiments as well as data analysis on various instruments provided by market leading companies.

A poster session/welcome reception was held on the first evening (Monday, July 16) to allow participants to meet instructors.

Practical sessions

The course schedule included 4 practical sessions of 120 minutes each. The sessions covered different experimental F-techniques on different instruments. Participants where divided into groups that rotated through the experimental stations.

Experimental techniques covered:

• FCS, FCCS and FLCS
• FLIM
• FRAP, AP-FRET
• FLIM-frequency domain

Course instructors

• Sohail Ahmed (IMB, Singapore)
• Andrew Clayton (Swinburne University, Australia)
• Srivats Hariharan (IMB, Singapore)
• Andreas Herrmann (Humboldt University Berlin, Germany)
• Peter Kapusta (J. Heyrovsky Institute, Czech Republic)
• Thomas Korte (Humboldt University Berlin, Germany)
• Radek Machán (J. Heyrovsky Institute, Czech Republic)
• Sudipta Maiti (Tata Institute of Fundamental Research, India)
• Steffen Ruettinger (PicoQuant GmbH, Germany)
• Markus Sauer (Julius-Maximilians-University Würzburg, Germany)
• Jean-Baptiste Sibarita (Bordeaux University, France)
• Sudha Thankiah (IMB, Singapore)
• Malte Wachsmuth (EMBL, Germany)
• Thorsten Wohland (NUS, Singapore)
• Graham Wright (IMB, Singapore)
• Qing-Hua Xu (NUS, Singapore)

Program

The program with all speakers and topics is still available for download as a PDF document.

• Time schedule of the workshop in Singapore 2012

Course impressions

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Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

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