South-East Asian Course on F-techniques
2nd Southeast Asian Course on "F-techniques: FCS, FCCS, FLCS, FRET, FLIM and FRAP"
July 16 - 20, 2012 in Singapore
Organized by Institute of Medical Biology Microscopy Unit (IMU), Biopolis, Singapore and PicoQuant GmbH, Germany

Aims and purpose
The course was intended for individuals wishing an in-depth introduction to the F-techniques and their application to the life sciences. Participants where normally graduate students or postdocs who are using fluorescence microscopy in their research. Most participants had some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, got the opportunity to enter this exciting field in a very effective way and benefited especially from the experimental section.
Course organisation
The course consisted of lectures and practical sessions.
The lectures on Monday and Tuesday (July 16/17) dealt with fluorescence, theory of F-techniques (FRET, FLIM, FCS and FRAP), FCS variants, instrumentation and data analysis. The major FRET methods where be covered in detail. The lectures on Wednesday and Thursday (July 18/19) covered research applications of F-techniques in cell and developmental biology. On Friday (July 20) lectures focused on superresolution microscopy, PALM, STORM and structured illumination.
Practical sessions (afternoons of July 17-20) covered steady-state and time-resolved experiments as well as data analysis on various instruments provided by market leading companies.
A poster session/welcome reception was held on the first evening (Monday, July 16) to allow participants to meet instructors.
Practical sessions
The course schedule included 4 practical sessions of 120 minutes each. The sessions covered different experimental F-techniques on different instruments. Participants where divided into groups that rotated through the experimental stations.
Experimental techniques covered:
- FCS, FCCS and FLCS
- FLIM
- FRAP, AP-FRET
- FLIM-frequency domain
Course instructors
- Sohail Ahmed (IMB, Singapore)
- Andrew Clayton (Swinburne University, Australia)
- Srivats Hariharan (IMB, Singapore)
- Andreas Herrmann (Humboldt University Berlin, Germany)
- Peter Kapusta (J. Heyrovsky Institute, Czech Republic)
- Thomas Korte (Humboldt University Berlin, Germany)
- Radek Machán (J. Heyrovsky Institute, Czech Republic)
- Sudipta Maiti (Tata Institute of Fundamental Research, India)
- Steffen Ruettinger (PicoQuant GmbH, Germany)
- Markus Sauer (Julius-Maximilians-University Würzburg, Germany)
- Jean-Baptiste Sibarita (Bordeaux University, France)
- Sudha Thankiah (IMB, Singapore)
- Malte Wachsmuth (EMBL, Germany)
- Thorsten Wohland (NUS, Singapore)
- Graham Wright (IMB, Singapore)
- Qing-Hua Xu (NUS, Singapore)
Program
The program with all speakers and topics is still available for download as a PDF document.
Archive
The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.
Science in Your Lab - Missoula 2019
July 30 - 31, 2019 | Missoula, MT, USA
Science in Your Lab - Vienna 2019
April 5, 2019 | Vienna, Austria
Science in Your Lab - Frederiksberg 2019
March 15, 2019 | Frederiksberg, Denmark
Science in Your Lab - Vienna 2019
January 17, 2019 | Vienna, Austria
Science in Your Lab - Moscow 2018
December 11, 2018 | Moscow, Russia
Science in Your Lab - Brno 2018
November 28, 2018 | Brno, Czech Republic
Science in your lab - Prague 2018
November 27, 2018 | Prague, Czech Republic
Science in your lab - Vestec 2018
November 26, 2018 | Vestec, Czech Republic
Single day workshop - Erlangen 2018
October 22, 2018 | Erlangen, Germany
Science in your lab - Zürich 2018
October 17, 2018 | Zürich, Switzerland
Seminar on the MicroTime 200
August 11, 2017 | Tianjin, China
Science in your labs - St. Louis 2016
December 2, 2016 | St. Louis, MO, USA
Science in your labs - Moscow 2016
October 12, 2016 | Moscow, Russia
FluoTime 300 Roadshow in Poland 2016
May 30 - June 3, 2016 | Krakow, Poland
FluoTime 300 Roadshow in China 2016
May 11, 2016 | Wuhan, China
Science in your labs - Daegu 2015
July 6, 2015 | Daegu, South Korea
FluoTime 300 Roadshow in Poland
May 26 - 29, 2015 | Chorzow, Wroclaw, Poland
Science in your labs - Mexico 2015
May 18 - 20, 2015 | Mexico City/Cuernavaca/San Luis Potosi, Mexico
Science in your labs - São Paulo 2015
April 17, 2015 | São Paulo, Brazil
Science in your labs - Lausanne 2015
March 17 - 18, 2015 | Lausanne, Switzerland
Science in your labs - St. Louis 2014
December 11, 2014 | St. Louis, MO, USA
Science in your labs - Vienna 2014
November 18 - 19, 2014 | Vienna, Austria
Science in your labs - Wuhan 2014
June 18, 2014 | Wuhan, China
Seminar on Fluorescence Spectroscopy - Warsaw, Poland
June 3, 2014 | Warsaw, Poland
Science in your labs - St. Louis 2013
December 12, 2013 | St. Louis, MO, USA
Science in your labs - Vienna 2013
November 12 - 13, 2013 | Vienna, Austria
Science in your labs - Liverpool
October 11, 2013 | Liverpool, United Kingdom
Science in your labs - San Diego
June 10, 2013 | San Diego, USA
Mini-Symposium on Fluorescence Spectroscopy
September 28, 2012 | Houston, USA
South-East Asian Course on F-techniques
July 16 - 20, 2012 | Singapore, Singapore
Science in your labs - Vienna
June 19 - 20, 2012 | Vienna, Austria
Workshop on advanced fluorescence spectroscopy
January 19, 2012 | Los Angeles, USA
South-East Asian Course on F-techniques
June 20 - 24, 2011 | Singapore, Singapore
Mini-Symposium on Fluorescence Spectroscopy
May 27, 2011 | Munich, Germany
PicoQuant Roadshow in China
May 9 - 19, 2011 | China
Mini-Symposium on Fluorescence Spectroscopy
March 4, 2011 | Baltimore, USA
Workshop on advanced fluorescence spectroscopy
January 10, 2011 | Los Angeles, USA
Single Molecule Fluorescence Workshop
April 26 - 28, 2010 | BNL, USA
Single Molecule Fluorescence Workshop
January 22 - 23, 2009 | Sacramento, USA
Single Molecule Fluorescence Workshop
January 17 - 18, 2008 | Sacramento, USA
Time-Resolved Spectroscopy for Bioanalytical Applications
August 30, 2007 | Fort Worth, USA
Single Molecule Fluorescence Workshop
January 18 - 19, 2007 | Sacramento, USA
Single Molecule Fluorescence Workshop
January 19 - 20, 2006 | Sacramento, USA
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