South-East Asian Course on F-techniques
2nd Southeast Asian Course on "F-techniques: FCS, FCCS, FLCS, FRET, FLIM and FRAP"
July 16 - 20, 2012 in Singapore
Organized by Institute of Medical Biology Microscopy Unit (IMU), Biopolis, Singapore and PicoQuant GmbH, Germany
Aims and purpose
The course was intended for individuals wishing an in-depth introduction to the F-techniques and their application to the life sciences. Participants where normally graduate students or postdocs who are using fluorescence microscopy in their research. Most participants had some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, got the opportunity to enter this exciting field in a very effective way and benefited especially from the experimental section.
The course consisted of lectures and practical sessions.
The lectures on Monday and Tuesday (July 16/17) dealt with fluorescence, theory of F-techniques (FRET, FLIM, FCS and FRAP), FCS variants, instrumentation and data analysis. The major FRET methods where be covered in detail. The lectures on Wednesday and Thursday (July 18/19) covered research applications of F-techniques in cell and developmental biology. On Friday (July 20) lectures focused on superresolution microscopy, PALM, STORM and structured illumination.
Practical sessions (afternoons of July 17-20) covered steady-state and time-resolved experiments as well as data analysis on various instruments provided by market leading companies.
A poster session/welcome reception was held on the first evening (Monday, July 16) to allow participants to meet instructors.
The course schedule included 4 practical sessions of 120 minutes each. The sessions covered different experimental F-techniques on different instruments. Participants where divided into groups that rotated through the experimental stations.
Experimental techniques covered:
- FCS, FCCS and FLCS
- FRAP, AP-FRET
- FLIM-frequency domain
- Sohail Ahmed (IMB, Singapore)
- Andrew Clayton (Swinburne University, Australia)
- Srivats Hariharan (IMB, Singapore)
- Andreas Herrmann (Humboldt University Berlin, Germany)
- Peter Kapusta (J. Heyrovsky Institute, Czech Republic)
- Thomas Korte (Humboldt University Berlin, Germany)
- Radek Machán (J. Heyrovsky Institute, Czech Republic)
- Sudipta Maiti (Tata Institute of Fundamental Research, India)
- Steffen Ruettinger (PicoQuant GmbH, Germany)
- Markus Sauer (Julius-Maximilians-University Würzburg, Germany)
- Jean-Baptiste Sibarita (Bordeaux University, France)
- Sudha Thankiah (IMB, Singapore)
- Malte Wachsmuth (EMBL, Germany)
- Thorsten Wohland (NUS, Singapore)
- Graham Wright (IMB, Singapore)
- Qing-Hua Xu (NUS, Singapore)
The program with all speakers and topics is still available for download as a PDF document.
The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.
January 17, 2019 | Vienna, Austria
December 11, 2018 | Moscow, Russia
November 28, 2018 | Brno, Czech Republic
November 27, 2018 | Prague, Czech Republic
November 26, 2018 | Vestec, Czech Republic
October 22, 2018 | Erlangen, Germany
October 17, 2018 | Zürich, Switzerland
August 11, 2017 | Tianjin, China
December 2, 2016 | St. Louis, MO, USA
October 12, 2016 | Moscow, Russia
May 30 - June 3, 2016 | Krakow, Poland
May 11, 2016 | Wuhan, China
July 6, 2015 | Daegu, South Korea
May 26 - 29, 2015 | Chorzow, Wroclaw, Poland
May 18 - 20, 2015 | Mexico City/Cuernavaca/San Luis Potosi, Mexico
April 17, 2015 | São Paulo, Brazil
March 17 - 18, 2015 | Lausanne, Switzerland
December 11, 2014 | St. Louis, MO, USA
November 18 - 19, 2014 | Vienna, Austria
June 18, 2014 | Wuhan, China
June 3, 2014 | Warsaw, Poland
December 12, 2013 | St. Louis, MO, USA
November 12 - 13, 2013 | Vienna, Austria
October 11, 2013 | Liverpool, United Kingdom
June 10, 2013 | San Diego, USA
September 28, 2012 | Houston, USA
July 16 - 20, 2012 | Singapore, Singapore
June 19 - 20, 2012 | Vienna, Austria
January 19, 2012 | Los Angeles, USA
June 20 - 24, 2011 | Singapore, Singapore
May 27, 2011 | Munich, Germany
May 9 - 19, 2011 | China
March 4, 2011 | Baltimore, USA
January 10, 2011 | Los Angeles, USA
April 26 - 28, 2010 | BNL, USA
January 22 - 23, 2009 | Sacramento, USA
January 17 - 18, 2008 | Sacramento, USA
August 30, 2007 | Fort Worth, USA
January 18 - 19, 2007 | Sacramento, USA
January 19 - 20, 2006 | Sacramento, USA
Thank you for registering for the South-East Asian Course on F-techniques!
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