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South-East Asian Course on F-techniques

2nd Southeast Asian Course on "F-techniques: FCS, FCCS, FLCS, FRET, FLIM and FRAP"

July 16 - 20, 2012 in Singapore


Organized by Institute of Medical Biology Microscopy Unit (IMU), Biopolis, Singapore and PicoQuant GmbH, Germany

Image South-East Asian Course on F-techniques 2012

Aims and purpose

The course was intended for individuals wishing an in-depth introduction to the F-techniques and their application to the life sciences. Participants where normally graduate students or postdocs who are using fluorescence microscopy in their research. Most participants had some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, got the opportunity to enter this exciting field in a very effective way and benefited especially from the experimental section.

Course organisation

The course consisted of lectures and practical sessions.

The lectures on Monday and Tuesday (July 16/17) dealt with fluorescence, theory of F-techniques (FRET, FLIM, FCS and FRAP), FCS variants, instrumentation and data analysis. The major FRET methods where be covered in detail. The lectures on Wednesday and Thursday (July 18/19) covered research applications of F-techniques in cell and developmental biology. On Friday (July 20) lectures focused on superresolution microscopy, PALM, STORM and structured illumination.

Practical sessions (afternoons of July 17-20) covered steady-state and time-resolved experiments as well as data analysis on various instruments provided by market leading companies.

A poster session/welcome reception was held on the first evening (Monday, July 16) to allow participants to meet instructors.

Practical sessions

The course schedule included 4 practical sessions of 120 minutes each. The sessions covered different experimental F-techniques on different instruments. Participants where divided into groups that rotated through the experimental stations.

Experimental techniques covered:

  • FCS, FCCS and FLCS
  • FLIM
  • FRAP, AP-FRET
  • FLIM-frequency domain

Course instructors

  • Sohail Ahmed (IMB, Singapore)
  • Andrew Clayton (Swinburne University, Australia)
  • Srivats Hariharan (IMB, Singapore)
  • Andreas Herrmann (Humboldt University Berlin, Germany)
  • Peter Kapusta (J. Heyrovsky Institute, Czech Republic)
  • Thomas Korte (Humboldt University Berlin, Germany)
  • Radek Machán (J. Heyrovsky Institute, Czech Republic)
  • Sudipta Maiti (Tata Institute of Fundamental Research, India)
  • Steffen Ruettinger (PicoQuant GmbH, Germany)
  • Markus Sauer (Julius-Maximilians-University Würzburg, Germany)
  • Jean-Baptiste Sibarita (Bordeaux University, France)
  • Sudha Thankiah (IMB, Singapore)
  • Malte Wachsmuth (EMBL, Germany)
  • Thorsten Wohland (NUS, Singapore)
  • Graham Wright (IMB, Singapore)
  • Qing-Hua Xu (NUS, Singapore)

Program

The program with all speakers and topics is still available for download as a PDF document.

Course impressions

 

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

Science in Your Lab - Missoula 2019

July 30 - 31, 2019 | Missoula, MT, USA


Science in Your Lab - Vienna 2019

April 5, 2019 | Vienna, Austria


Science in Your Lab - Frederiksberg 2019

March 15, 2019 | Frederiksberg, Denmark


Science in Your Lab - Vienna 2019

January 17, 2019 | Vienna, Austria


Science in Your Lab - Moscow 2018

December 11, 2018 | Moscow, Russia


Science in Your Lab - Brno 2018

November 28, 2018 | Brno, Czech Republic


Science in your lab - Prague 2018

November 27, 2018 | Prague, Czech Republic


Science in your lab - Vestec 2018

November 26, 2018 | Vestec, Czech Republic


Single day workshop - Erlangen 2018

October 22, 2018 | Erlangen, Germany


Science in your lab - Zürich 2018

October 17, 2018 | Zürich, Switzerland


Seminar on the MicroTime 200

August 11, 2017 | Tianjin, China


Science in your labs - St. Louis 2016

December 2, 2016 | St. Louis, MO, USA


Science in your labs - Moscow 2016

October 12, 2016 | Moscow, Russia


FluoTime 300 Roadshow in Poland 2016

May 30 - June 3, 2016 | Krakow, Poland


FluoTime 300 Roadshow in China 2016

May 11, 2016 | Wuhan, China


Science in your labs - Daegu 2015

July 6, 2015 | Daegu, South Korea


FluoTime 300 Roadshow in Poland

May 26 - 29, 2015 | Chorzow, Wroclaw, Poland


Science in your labs - Mexico 2015

May 18 - 20, 2015 | Mexico City/Cuernavaca/San Luis Potosi, Mexico


Science in your labs - São Paulo 2015

April 17, 2015 | São Paulo, Brazil


Science in your labs - Lausanne 2015

March 17 - 18, 2015 | Lausanne, Switzerland


Science in your labs - St. Louis 2014

December 11, 2014 | St. Louis, MO, USA


Science in your labs - Vienna 2014

November 18 - 19, 2014 | Vienna, Austria


Science in your labs - Wuhan 2014

June 18, 2014 | Wuhan, China



Science in your labs - St. Louis 2013

December 12, 2013 | St. Louis, MO, USA


Science in your labs - Vienna 2013

November 12 - 13, 2013 | Vienna, Austria


Science in your labs - Liverpool

October 11, 2013 | Liverpool, United Kingdom


Science in your labs - San Diego

June 10, 2013 | San Diego, USA


Mini-Symposium on Fluorescence Spectroscopy

September 28, 2012 | Houston, USA


South-East Asian Course on F-techniques

July 16 - 20, 2012 | Singapore, Singapore


Science in your labs - Vienna

June 19 - 20, 2012 | Vienna, Austria


Workshop on advanced fluorescence spectroscopy

January 19, 2012 | Los Angeles, USA


South-East Asian Course on F-techniques

June 20 - 24, 2011 | Singapore, Singapore


Mini-Symposium on Fluorescence Spectroscopy

May 27, 2011 | Munich, Germany


PicoQuant Roadshow in China

May 9 - 19, 2011 | China


Mini-Symposium on Fluorescence Spectroscopy

March 4, 2011 | Baltimore, USA


Workshop on advanced fluorescence spectroscopy

January 10, 2011 | Los Angeles, USA


Single Molecule Fluorescence Workshop

April 26 - 28, 2010 | BNL, USA


Single Molecule Fluorescence Workshop

January 22 - 23, 2009 | Sacramento, USA


Single Molecule Fluorescence Workshop

January 17 - 18, 2008 | Sacramento, USA



Single Molecule Fluorescence Workshop

January 18 - 19, 2007 | Sacramento, USA


Single Molecule Fluorescence Workshop

January 19 - 20, 2006 | Sacramento, USA


 

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