PicoQuant: Science in your labs
“3rd FLIM and FCS Workshop”

Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) are particularly well suited to monitor interactions between molecules both in cells and in vitro. Yet, the 'activation energy' to apply these methods in life science research is relatively high. That is why one of the leading manufacturer of advanced imaging instruments PicoQuant, Vienna BioCenter Campus Science Support Facilities GmbH (CSF) and Max F. Perutz Laboratories (MFPL), which is a joint venture of the University of Vienna and Medical University of Vienna, joined efforts to organize the 3rd FLIM & FCS Workshop.

The workshop showcased the utility of the advanced imaging techniques in biological research and provide hands-on practicals using real samples in order to lower the 'activation barrier' for the potential users. Leading scientists introduced the concepts and applications of time-resolved fluorescence microscopy, fluorescence auto- and cross-correlation and anisotropy measurements, super-resolution imaging. Examples of their applications in biological research were demonstrated in the afternoon. Practical sessions also covered various aspects of sample preparation, data acquisition, and analysis. The program was completed by a poster session.

Speakers

• Volker Buschmann (PicoQuant GmbH, Germany)
  “With Photon Patterns Towards Species Selective Microscopy”
• Paul French (Imperial College London, United Kingdom)
  “Fluorescence Lifetime Imaging Across the Scales”
• Thomas Ganz (FEMTOLASERS, Austria)
  “Multi-model Imaging Using a Noval and Portable Ultrafast Light Source”
• Erwin Gaubitzer (MFPL, Austria)
  “A Short History of Fluorescence Fluctuations”
• Enrico Gratton (University of California, Irvine, USA)
  “The iMSD method for imaging FCS”
• Mark Hink (University of Amsterdam, The Netherlands)
  “Analysing noise: Quantitative cell biology by using Fluorescence Fluctuation Spectroscopy”
• Jörg Langowski (DKFZ, Germany)
  “Live Maps of Protein Mobility by Light Sheet FCS”
• Gregor Klatt (Laser Quantum, Germany)
  “Laser solutions for life science applications ”
• Bernd Müller-Zülow (LaVision BioTec, Germany)
  “A fully integrated multi-photon based FLIM-System ”
• Ulrich Nienhaus (Karslruhe Institute of Technology, Germany)
  “Novel Fluctucation Correlation Spectroscopy Techniques for a Wide Range of Applications”
• Sandra Orthaus-Müller (PicoQuant GmbH, Germany)
  “Advanced Fluorescence Lifetime Imaging Microscopy (FLIM): Concepts and Applications”
• Ivan Yudushkin (Max F. Perutz Laboratories, Austria)
  “Design of FRET probes”

Time schedule

Venue: IMP Main Lecture Hall, Dr. Bohr-Gasse 7, 1030 Vienna, Austria

Tuesday, November 18
08:00 - 09:00	Registration
09:00 - 9:15	Welcoming address Ivan Yudushkin and the Scientific Director (MFPL) Kareem Elsayad (CSF)
9:15 - 9:45	The iMSD method for imaging FCS Enrico Gratton (University of California)
9:45 - 10:45	Analysing noise: Quantitative cell biology by using Fluorescence Fluctuation Spectroscopy Mark Hink (University of Amsterdam)
10:45 - 11:30	Coffee break and poster presentation
11:30 - 12:00	A Short History of Fluorescence Fluctuations Erwin Gaubitzer (MFPL)
12:00 - 12:30	With Photon Patterns Towards Species Selective Microscopy Volker Buschmann (PicoQuant GmbH)
12:30 - 12:45	Multi-model Imaging Using a Noval and Portable Ultrafast Light Source Thomas Ganz (Femtolaser)
12:45 - 13:00	Group picture
13:00 - 14:00	Lunch break
14:00 - 16:00	Practical sessions (by appointment) MicroTime 200 (FLIM/FCS/Anisotropy) DeltaVision OMX (3D structured illumination) Light-sheet Brillouin scattering confocal microscopy Choice of fluorophores and sample preparation
16:00 - 16:30	Coffee break and poster presentation
16:30 - 18:30	Practical sessions (by appointment) MicroTime 200 (FLIM/FCS/Anisotropy) DeltaVision OMX (3D structured illumination) Light-sheet Brillouin scattering confocal microscopy Choice of fluorophores and sample preparation
Wednesday, November 19
9:00 - 9:30	Fluorescence Lifetime Imaging Across the Scales Paul French (Imperial College London)
9:30 - 10:00	Novel Fluctuation Correlation Spectrosocopy Techniques for a Wide Range of Applications Ulrich Nienhaus (Karlsruhe Institute of Technology)
10:00 - 10:30	Live Maps of Protein Mobility by Light Sheet FCS Jörg Langowski (DKFZ)
10:30 - 11:15	Coffee break and poster presentation
11:15 - 11:30	Laser solutions for life science applications Gregor Klatt (Laser Quantum)
11:30 - 12:00	Advanced Fluorescence Lifetime Imaging Microscopy (FLIM): Concepts and Applications Sandra Orthaus-Müller (PicoQuant GmbH)
12:00 - 12:30	Design of FRET probes Ivan Yudushkin (MFPL)
12:30 - 12:45	A fully integrated multi-photon based FLIM-System Bernd Müller-Zülow (LaVision BioTec)
12:45 - 13:00	Closing statements Ivan Yudushkin (MFPL)
13:00 - 14:00	Lunch break
14:00 - 16:00	Practical sessions (by appointment) MicroTime 200 (FLIM/FCS/Anisotropy) DeltaVision OMX (3D structured illumination) Light-sheet Brillouin scattering confocal microscopy Choice of fluorophores and sample preparation
16:00 - 16:30	Coffee break and poster presentation
16:30 - 18:30	Practical sessions (by appointment) MicroTime 200 (FLIM/FCS/Anisotropy) DeltaVision OMX (3D structured illumination) Light-sheet Brillouin scattering confocal microscopy Choice of fluorophores and sample preparation

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

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