PicoQuant: Science in your labs
“2nd FLIM and FCS Workshop”

Summary

Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) are particularly well suited to monitor interactions between molecules both in cells and in vitro. Yet, the 'activation energy' to apply these methods in life science research is relatively high. That is why one of the leading manufacturers of advanced imaging instruments, PicoQuant, Vienna BioCenter Campus Scientific Facility (CSF) and Max F. Perutz Laboratories (MFPL), which is a joint venture of the University of Vienna and Medical University of Vienna, had joined efforts to organize the 2nd MFPL FLIM and FCS Workshop. It was held on November 12-13, 2013 in Vienna, Austria.

The workshop showcased the utility of the advanced imaging techniques in biological research and provided hands-on practicals using real samples in order to lower the 'activation barrier' for the potential users. Outstanding scientists, including Gottfried Köhler (MFPL, Austria), Katrin Heinze (University of Würzburg, Germany), Gerhard Schütz (Technical University of Vienna, Austria) and Sofia Johansson (Karolinska Institutet, Sweden) introduced the concepts of time-resolved fluorescence microscopy, fluorescence auto- and cross-correlation and anisotropy measurements, superresolution imaging, development and use of fluorescent reporters, and the examples of their use in biological research.

In the afternoon, scientists from the MFPL, CSF, and PicoQuant demonstrated FLIM, FCS, and structural illumination using real biological samples. These hands-on sessions covered various practical aspects of sample preparation, data acquisition and analysis using PicoQuant's MicroTime 200 and Applied Precision's DeltaVision OMX systems available via CSF Advanced Microscopy group.

Information and correspondence

Speakers

• Kareem Elsayad (Vienna BioCenter Campus Scientific Facility, Austria)
  “Getting and making the most out of fluorescence lifetimes”
• Katrin Heinze (University of Würzburg, Germany)
  "Facts and Artifacts in High Resolution Fluorescence Imaging and Spectroscopy"
• Sofia Johansson (Karolinska Institutet, Sweden)
  “Dynamics of Natural Killer cell receptors and ligands at the cell surface”
• Gottfried Köhler (Max F. Perutz Laboratories, Austria)
  “Is Virus Uncoating directional? An example for use of FCS”
• Edmundo Sanchez Guajardo (Vienna BioCenter Campus Scientific Facility, Austria)
  “Microscopes at the CSF: current and planned
• Gerhard Schütz (Technical University of Vienna, Austria)
  “Tracking single molecules in the live cell plasma membrane”
• Felix Koberling (PicoQuant GmbH, Germany)
  "Current Concepts for Time- and Polarization-Resolved Fluorescence Imaging"
• Susanne Trautmann (PicoQuant GmbH, Germany)
  "An introduction to advances in Fluorescence Correlation Spectroscopy (FCS): applications and technology"
• Ivan Yudushkin (Max F. Perutz Laboratories, Austria)
  “FLIM for biologists: a showcase” and “Design of FRET probes”

Time schedule

The time schedule of the course is also available for download.

Tuesday, November 12 Venue: IMP lecture hall, entrance via Max F. Perutz Laboratories, Dr. Bohr-Gasse 9, 1030 Vienna
09:00 - 09:30	FLIM for biologists: a showcase Ivan Yudushkin (MFPL)
09:30 - 10:00	Tracking single molecules in the live cell plasma membrane Gerhard Schütz (TU Vienna)
10:00 - 10:30	Getting and making the most out of fluorescence lifetimes Kareem Elsayad (CSF)
10:30 - 11:00	Coffee break
11:00 - 11:30	Dynamics of Natural Killer cell receptors and ligands at the cell surface Sofia Johansson (Karolinska Institutet)
11:30 - 12:00	Current Concepts for Time- and Polarization-Resolved Fluorescence Imaging Felix Koberling (PicoQuant GmbH)
12:00 - 13:00	Lunch (IMP cafeteria)
13:00 - 17:00	Demo sessions (by appointment) MicroTime 200 (FLIM/FCS/Anisotropy) DeltaVision OMX (structured illumination) Choice of fluorophores and sample preparation
17:00 - 18:00	Open discussion Social event
Wednesday, November 13 Venue: Max F. Perutz Laboratories, 6th floor, seminar room 6.506, Dr. Bohr-Gasse 9, 1030 Vienna
09:00 - 09:30	Facts and Artifacts in High Resolution Fluorescence Imaging and Spectroscopy Katrin Heinze (University of Würzburg)
09:30 - 10:00	Is Virus Uncoating directional? An example for use of FCS Gottfried Köhler (MFPL)
10:00 - 10:30	An introduction to advances in Fluorescence Correlation Spectroscopy (FCS): applications and technology Susanne Trautmann (PicoQuant GmbH)
10:30 - 11:00	Coffee break
11:00 - 11:30	Microscopes at the CSF: current and planned Edmundo Sanchez Guajardo (CSF)
11:30 - 12:00	Design of FRET probes Ivan Yudushkin (MFPL)
12:00 - 13:00	Lunch (IMP cafeteria)
13:00 - 17:00	Demo sessions (by appointment) MicroTime 200 (FLIM/FCS/Anisotropy) DeltaVision OMX (structured illumination)
17:00 - 18:00	Open discussion Social event

 

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

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