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Science in your labs - Vienna 2013

PicoQuant: Science in your labs
“2nd FLIM and FCS Workshop”

November 12 - 13, 2013 in Vienna, Austria


Joint workshop of Max F. Perutz Laboratories and PicoQuant GmbH

Image Science in your labs - Vienna 2013 2013

Summary

Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) are particularly well suited to monitor interactions between molecules both in cells and in vitro. Yet, the 'activation energy' to apply these methods in life science research is relatively high. That is why one of the leading manufacturers of advanced imaging instruments, PicoQuant, Vienna BioCenter Campus Scientific Facility (CSF) and Max F. Perutz Laboratories (MFPL), which is a joint venture of the University of Vienna and Medical University of Vienna, had joined efforts to organize the 2nd MFPL FLIM and FCS Workshop. It was held on November 12-13, 2013 in Vienna, Austria.

The workshop showcased the utility of the advanced imaging techniques in biological research and provided hands-on practicals using real samples in order to lower the 'activation barrier' for the potential users. Outstanding scientists, including Gottfried Köhler (MFPL, Austria), Katrin Heinze (University of Würzburg, Germany), Gerhard Schütz (Technical University of Vienna, Austria) and Sofia Johansson (Karolinska Institutet, Sweden) introduced the concepts of time-resolved fluorescence microscopy, fluorescence auto- and cross-correlation and anisotropy measurements, superresolution imaging, development and use of fluorescent reporters, and the examples of their use in biological research.

In the afternoon, scientists from the MFPL, CSF, and PicoQuant demonstrated FLIM, FCS, and structural illumination using real biological samples. These hands-on sessions covered various practical aspects of sample preparation, data acquisition and analysis using PicoQuant's MicroTime 200 and Applied Precision's DeltaVision OMX systems available via CSF Advanced Microscopy group.

Information and correspondence

PicoQuant GmbH
Jana Rudolph
Rudower Chaussee 29
12489 Berlin, Germany
Tel: +49-30-6392-6929
Email: events@picoquant.com

Max F. Perutz Laboratories (MFPL)
Ivan Yudushkin
Dr. Bohr-Gasse 9
1030 Vienna, Austria
Tel.: +43-1-4277-61672
Email: ivan.yudushkin@mfpl.ac.at

Speakers

  • Kareem Elsayad (Vienna BioCenter Campus Scientific Facility, Austria)
    “Getting and making the most out of fluorescence lifetimes”
  • Katrin Heinze (University of Würzburg, Germany)
    "Facts and Artifacts in High Resolution Fluorescence Imaging and Spectroscopy"
  • Sofia Johansson (Karolinska Institutet, Sweden)
    “Dynamics of Natural Killer cell receptors and ligands at the cell surface”
  • Gottfried Köhler (Max F. Perutz Laboratories, Austria)
    “Is Virus Uncoating directional? An example for use of FCS”
  • Edmundo Sanchez Guajardo (Vienna BioCenter Campus Scientific Facility, Austria)
    “Microscopes at the CSF: current and planned
  • Gerhard Schütz (Technical University of Vienna, Austria)
    “Tracking single molecules in the live cell plasma membrane”
  • Felix Koberling (PicoQuant GmbH, Germany)
    "Current Concepts for Time- and Polarization-Resolved Fluorescence Imaging"
  • Susanne Trautmann (PicoQuant GmbH, Germany)
    "An introduction to advances in Fluorescence Correlation Spectroscopy (FCS): applications and technology"
  • Ivan Yudushkin (Max F. Perutz Laboratories, Austria)
    “FLIM for biologists: a showcase” and “Design of FRET probes”

Time schedule

The time schedule of the course is also available for download.

Tuesday, November 12
Venue: IMP lecture hall, entrance via Max F. Perutz Laboratories, Dr. Bohr-Gasse 9, 1030 Vienna
09:00 - 09:30 FLIM for biologists: a showcase
Ivan Yudushkin (MFPL)
09:30 - 10:00 Tracking single molecules in the live cell plasma membrane
Gerhard Schütz (TU Vienna)
10:00 - 10:30 Getting and making the most out of fluorescence lifetimes
Kareem Elsayad (CSF)
10:30 - 11:00 Coffee break
11:00 - 11:30 Dynamics of Natural Killer cell receptors and ligands at the cell surface
Sofia Johansson (Karolinska Institutet)
11:30 - 12:00 Current Concepts for Time- and Polarization-Resolved Fluorescence Imaging
Felix Koberling (PicoQuant GmbH)
12:00 - 13:00 Lunch (IMP cafeteria)
13:00 - 17:00 Demo sessions (by appointment)
MicroTime 200 (FLIM/FCS/Anisotropy)
DeltaVision OMX (structured illumination)
Choice of fluorophores and sample preparation
17:00 - 18:00

Open discussion
Social event

Wednesday, November 13
Venue: Max F. Perutz Laboratories, 6th floor, seminar room 6.506, Dr. Bohr-Gasse 9, 1030 Vienna
09:00 - 09:30 Facts and Artifacts in High Resolution Fluorescence Imaging and Spectroscopy
Katrin Heinze (University of Würzburg)
09:30 - 10:00 Is Virus Uncoating directional? An example for use of FCS
Gottfried Köhler (MFPL)
10:00 - 10:30 An introduction to advances in Fluorescence Correlation Spectroscopy (FCS): applications and technology
Susanne Trautmann (PicoQuant GmbH)
10:30 - 11:00 Coffee break
11:00 - 11:30 Microscopes at the CSF: current and planned
Edmundo Sanchez Guajardo (CSF)
11:30 - 12:00 Design of FRET probes
Ivan Yudushkin (MFPL)
12:00 - 13:00 Lunch (IMP cafeteria)
13:00 - 17:00 Demo sessions (by appointment)
MicroTime 200 (FLIM/FCS/Anisotropy)
DeltaVision OMX (structured illumination)
17:00 - 18:00

Open discussion
Social event

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

Science in Your Lab - Vienna 2019

January 17, 2019 | Vienna, Austria


Science in Your Lab - Moscow 2018

December 11, 2018 | Moscow, Russia


Science in Your Lab - Brno 2018

November 28, 2018 | Brno, Czech Republic


Science in your lab - Prague 2018

November 27, 2018 | Prague, Czech Republic


Science in your lab - Vestec 2018

November 26, 2018 | Vestec, Czech Republic


Single day workshop - Erlangen 2018

October 22, 2018 | Erlangen, Germany


Science in your lab - Zürich 2018

October 17, 2018 | Zürich, Switzerland


Seminar on the MicroTime 200

August 11, 2017 | Tianjin, China


Science in your labs - St. Louis 2016

December 2, 2016 | St. Louis, MO, USA


Science in your labs - Moscow 2016

October 12, 2016 | Moscow, Russia


FluoTime 300 Roadshow in Poland 2016

May 30 - June 3, 2016 | Krakow, Poland


FluoTime 300 Roadshow in China 2016

May 11, 2016 | Wuhan, China


Science in your labs - Daegu 2015

July 6, 2015 | Daegu, South Korea


FluoTime 300 Roadshow in Poland

May 26 - 29, 2015 | Chorzow, Wroclaw, Poland


Science in your labs - Mexico 2015

May 18 - 20, 2015 | Mexico City/Cuernavaca/San Luis Potosi, Mexico


Science in your labs - São Paulo 2015

April 17, 2015 | São Paulo, Brazil


Science in your labs - Lausanne 2015

March 17 - 18, 2015 | Lausanne, Switzerland


Science in your labs - St. Louis 2014

December 11, 2014 | St. Louis, MO, USA


Science in your labs - Vienna 2014

November 18 - 19, 2014 | Vienna, Austria


Science in your labs - Wuhan 2014

June 18, 2014 | Wuhan, China



Science in your labs - St. Louis 2013

December 12, 2013 | St. Louis, MO, USA


Science in your labs - Vienna 2013

November 12 - 13, 2013 | Vienna, Austria


Science in your labs - Liverpool

October 11, 2013 | Liverpool, United Kingdom


Science in your labs - San Diego

June 10, 2013 | San Diego, USA


Mini-Symposium on Fluorescence Spectroscopy

September 28, 2012 | Houston, USA


South-East Asian Course on F-techniques

July 16 - 20, 2012 | Singapore, Singapore


Science in your labs - Vienna

June 19 - 20, 2012 | Vienna, Austria


Workshop on advanced fluorescence spectroscopy

January 19, 2012 | Los Angeles, USA


South-East Asian Course on F-techniques

June 20 - 24, 2011 | Singapore, Singapore


Mini-Symposium on Fluorescence Spectroscopy

May 27, 2011 | Munich, Germany


PicoQuant Roadshow in China

May 9 - 19, 2011 | China


Mini-Symposium on Fluorescence Spectroscopy

March 4, 2011 | Baltimore, USA


Workshop on advanced fluorescence spectroscopy

January 10, 2011 | Los Angeles, USA


Single Molecule Fluorescence Workshop

April 26 - 28, 2010 | BNL, USA


Single Molecule Fluorescence Workshop

January 22 - 23, 2009 | Sacramento, USA


Single Molecule Fluorescence Workshop

January 17 - 18, 2008 | Sacramento, USA



Single Molecule Fluorescence Workshop

January 18 - 19, 2007 | Sacramento, USA


Single Molecule Fluorescence Workshop

January 19 - 20, 2006 | Sacramento, USA


 

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