The workshop was held on the occasion of the installation of a new Zeiss confocal microscope LSM 780 with a PicoQuant fluorescence lifetime imaging attachment at the Centre for Cell Imaging (CCI). It included talks on new capabilities, potential applications and some demonstrations.

Lifetime measurements can be used for quantitative FRET, e.g., protein-protein interactions, and for environmental sensors. The new microscope also enables Fluorescence Correlation Spectroscopy for the measurement of diffusion time; i.e., protein complex formation.

Speakers

• Ali Kinkhabwala (Max Planck Institute of Molecular Physiology)
  "PTP1B's Journey to the Centre of the Mitochondria as Revealed by Advanced Light and Electron  Microscopy"
• Chris Powers (Carl Zeiss)
  "Imaging capabilities of the LSM 780"
• Manoel Veiga (PicoQuant GmbH)
  "Principles and Applications for Fluorescent Lifetime Imaging Microscopy (FLIM) and Fluorescence  Correlation Spectroscopy (FCS)"
• Tobias Zech (LITM Liverpool)
  "Spatiotemporal regulation of actin mediated invasive migration"

Schedule

Time	Topic
9:30 am	Welcome
09:45 am - 10:00 am	Introduction to PicoQuant
10:00 am - 10:30 am	Chris Powers "Imaging capabilities of the LSM 780"
10:30 am - 11:00 am	Manoel Veiga (PicoQuant GmbH) “Principles and Applications for Fluorescent Lifetime Imaging Microscopy (FLIM) and Fluorescence"
11:00 am - 11:20 am	Coffee break
11:20 am - 11:50 am	Tobias Zech (LITM Liverpool) “Spatiotemporal regulation of actin mediated invasive migration”
11:50 am - 12:00 pm	Group picture
12:00 pm - 1:00 pm	Lunch break
1:00 pm - 1:40 pm	Ali Kinkhabwala (Max Planck Institute of Molecular Physiology) “PTP1B's Journey to the Centre of the Mitochondria as Revealed by Advanced Light and Electron  Microscopy”
1:40 pm - 2:00 pm	Closing remarks and group split for demonstrations
2:00 pm - 4:00 pm	Demonstration of the Zeiss LSM 780 and the PicoQuant FLIM/FCS Upgrade

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

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