PicoQuant: Science in your labs
"Advanced fluorescence spectroscopy and microscopy: from cells to single molecules"

Summary

The single day event focussed on latest developments in single molecule and fluorescence lifetime measurements and their application even with / for cellular samples. The topics covered during this symposium included basic single molecule detection techniques, fluorescence microscopy, Fluorescence Lifetime Imaging (FLIM), Förster Resonance Energy Transfer (FRET), Fluorescence Correlation Spectroscopy (FCS), superresolution microscopy and other advanced fluorescence methods.

The aim of this symposium was to stimulate discussion in this field and to give an introduction to current research activities in the field of single molecule detection (SMD) and Fluorescence Lifetime Imaging (FLIM).

Invited Speakers

• Hu Cang (SALK Institute)
  "Cloud computing and light-sheet illumination for single-molecule super-resolution imaging"
• Michelle Digman (UC Irvine)
  "Multiplexing the pair correlation function (pCF) analysis with Fluorescence Lifetime Imaging Microscopy (FLIM) line scans to obtain spatial temporal maps of GTPase Activation"
• David Hall (UC San Diego)
  "In Vivo Fluorescence Lifetime Imaging Applications"
• David Millar (The Scripps Institute)
  "DNA Polymerase Activity at the Single-Molecule Level"
• Rainer Erdmann (PicoQuant GmbH)
  "Novel Applications for Time-, Spectrally and Polarisation Resolved Imaging with Standard Laser Scanning Microscopes"
  "Looking at Single Molecules - Time-resolved Correlation Spectroscopy and Confocal Microscopy"

Time Schedule

Time	Speaker	Title
9:30 am	Registration
10:00 am - 10:30 am	David Millar, Rainer Erdmann	Welcome and Introduction
10:30 am - 11:00 am	Rainer Erdmann	Novel Applications for Time-, Spectrally and Polarisation Resolved Imaging with Standard Laser Scanning Microscopes
11:00 am - 11:30 am	David Millar	DNA Polymerase Activity at the Single-Molecule Level
11:30 am - 12:00 pm	David Hall	In Vivo Fluorescence Lifetime Imaging Applications
12:00 pm - 12:10 am	Group picture
12:10 pm - 1:10 pm	Lunch break
1:10 pm - 1:40 pm	Michelle Digiman	Multiplexing the pair correlation function (pCF) analysis with Fluorescence Lifetime Imaging Microscopy (FLIM) line scans to obtain spatial temporal maps of GTPase Activation
1:40 pm - 2:10 pm	Rainer Erdmann	Looking at Single Molecules - Time-resolved Correlation Spectroscopy and Confocal Microscopy
2:10 pm - 2:40 pm	Hu Cang	Cloud computing and light-sheet illumination for single-molecule super-resolution imaging
2:40 pm - 2:55 pm	Rainer Erdmann	Closing Remarks
2:55 pm - 4:30 pm	Open discussion

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

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