v 2.9 (February 2025) - FlexWave Support for FLIMBee Skin - MultiHarp 4.0 Support - Bugfixes for japanese Windows Versions v 2.9 (August 2024) - FlexWave Support - Bugfix for slow Test mode on some computer - PicoHarp 300 Router Bugfix - PicoHarp 330 4P Support - Result file loading speedup v 2.8 (March 2024) - PicoHarp 330 Support - Bugfix for Focus Camera in FLIMBee Skin v 2.8 (Dec. 2023) new features: - PicoHarp 330 supported - IDS sideport camera U3-3990 supported - ASCII ROI import/export - manual adjustment of laser position during point measurements - minor Bugfixes v 2.7 (June 2021) new features: - MultiHarp 160 supported - adjustments for 64 channel measurements and analysis - additional antibunching functionality (separate version) - updated Sepia drivers => breaking changes - OME-TIFF export for images - update of HASP (Hardlock) drivers, addressing blue screen during installation - minor Bugfixes breaking changes: - new driver versions for Sepia needed, will disrupt following packages, if installed on same computer - EasyTau SW - ... or older versions of the Sepia software - (Backdated to v 2.5): - Changes to the Workspace as implemented in v2.5 may lead to compiling errors for some user created scripts v 2.6 (Aug. 2020) Known Problems: - Installation of the HASP driver for the hardlock license can lead to a blue screen crash of Windows. Fix: Please download the driver here: https://supportportal.thalesgroup.com/csm?sys_kb_id=61fb0ee1dbd2e78cfe0aff3dbf9619ab&id=kb_article_view&sysparm_rank=13&sysparm_tsqueryId=785639631b27dc50f2888739cd4bcbdb&sysparm_article=KB0018320 When installing this driver, please choose "repair" during the installation. Then install SymPhoTime 64 again, making sure that the "HASP Driver" checkbox is NOT ticked. Otherwise the faulty driver will be installed once more. new features: - MultiHarp 150 P with 10ps resolution supported - MT200: Implementation of successor for FrameGrabber - relevant only for future MT200 microscopes - minor bugfixes and improvements breaking changes: - (Backdated to v 2.5): - Changes to the Workspace as implemented in v2.5 may lead to compiling errors for some user created scripts v 2.5 (Mar. 2020) new features: - better operation with wide range scanner and FLIMBee/PI scanner in the same serup - x line scanning with FLIMBee - analysis for handling of files with many frames (Multi Frame BatchAnalysis) - side port camera in wide range scanner skin - FLIMBee: stop measurement when maximum counts in a pixel is reached - maximum acquisition time set to 100h - new more stable Workspace handling (breaks compatibility with user created scripts) - improvements for rapid FLIM - electrical pulse width estimated directly from data - improved memory management - improved processing time - fitting model for rapid FLIM and cyclic excitation - Python file demos - file demos for image files - Bugfixes v 2.4 (Sep. 2018) new features: - MultiHarp 150 supported - Bugfixes v 2.3.4724 (Nov. 2017) new features: - import of LIN camera files - bugfixes v 2.3 (Jul. 2017) new features: - rapidFLIM - new reconvoultion fitting models for correction of detector pulse pile up - FLIM - FRET - movie export for pattern matching/fast pattern matching - intensity distortions (bright sides) for sinusoidal scanning removed - PI scanning controller E727 supported - new controller C867 PI wide range scanner supported - new driver for FrameGrabber to enhance performance under WIN 10 - N.b.: can affect the brightness of the camera image. - bugfixes - especially concerning workspace stability - especially concerning FLIMbee v 2.2 (Nov. 2016) new features: - full support of the PQ FLIMbee galvo scanner - molecular brightness calculated in FCS fitting - group analysis for FLIM FRET - FLIM: import and export of ROIs - setting laser driver (Sepia) parameters via SPT remote control - TCSPC fitting for ROI decays in S/E histogram - FLCS: calculated IRF as a pattern - fast pattern matching: limitation to two patterns removed, now arbitrary number of patterns - improved direct excitation correction for PIE FRET - multi frame functionality for pattern matching - export of frame stacks as movies for pattern matching - ROIs for grouped FLIM analysis - bug fixes and minor improvements v 2.1.3813 (Sept. 2015) - bugfixes v 2.1 (Apr. 2015) new features: - PI725 controller supported - IDS uEye USB 3 - new MT200 Mainboard supported - AFM support - grid/tile for point/image measurements - global analysis for FCS and TCSPC curve fitting - STED - data acquisition - gated STED image analysis - STED + Confocal image analysis - advanced pulse pattern - fast pattern matching - resolution estimation - added to FLIM analysis - Lorentzian profile added - diagonal slices added - undo for ROIs - TCSPC binning for FLIM binary image exports - TIFF export - bugfixes