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| The TimeHarp 100 has served the community for seven years. It was used in pioneering single molecule work and hundreds of other time-resolved fluorescence experiments. As technology moves on, notably PCs no longer provide ISA-slots, we now have to discontinue this product. Small numbers are still on stock to meet requirements of replacement or installation in older PCs. Of course we still provide support for TimeHarp 100 customers. An upgrade path to newer hardware is available by means of the TimeHarp 200 PCI-board, providing very similar functionality and an almost identical software interface. |
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Features |
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Reverse Start Stop Mode |
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Dual memory architecture |
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4096 channels, min. width < 40 ps |
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6 measurement ranges up to 4500 ns |
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Count rate up to 3 million counts/sec |
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Continuous mode for unlimited sequential curve recording down to 1 ms per histogram |
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Easy-to-use software for all 32-bit Windows™ platforms |
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External Sync for lifetime imaging in continuous modem |
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Time-Tagged Time-Resolved (TTTR) mode recording of each event* |
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Drivers and demo code for custom programming in LabVIEW™ etc. |
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Applications |
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Time-resolved fluorescence and luminescence spectroscopy |
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Fluorescence lifetime imaging (FLIM) |
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Single molecule spectroscopy |
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Time response characterization of opto-electronic devices |
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Time-of-flight measurements |
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Coincidence correlation and antibunching |
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Optical time domain reflectometry (OTDR) |
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Optical tomography |
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Timing analysis |
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A Brief Description |
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The TimeHarp 100 PC-board is a complete system for picosecond time resolved measurements, ideally suited to work with the PDL 800-B as an excitation source. A solid state design keeps cost down, improves reliability and permits new applications. The system allows high measurement rates up to 3 million counts/sec and provides a time resolution under 40 ps. The system operates in reverse start-stop mode to allow synchronisation with fast laser sources. Both input triggers are programmable for a variety of signal types. The start input has a built in CFD for direct use of common photon detectors such as PMTs. A signal adaptor for connection of SPAD detectors is also available.
In combination with a pulsed diode laser total IRF widths as short as 300 ps FWHM permit the recording of sub-nanosecond fluorescence lifetimes, extendable to < 100 ps with reconvolution. For multi-exponential reconvolution the FluoFit software is an ideal tool.
The TimeHarp system can operate in various modes. Controlled by software, by a timer circuit or an external signal it is able to switch between 32 measurement curves. A sophisticated real-time RAM bank switching technique is used to prevent any dead times in continuous mode F(t,T). With an external TTL signal the recording can be synchronized to any scanner or fluorescence microscope for Fluorescence Lifetime Imaging (FLIM). The TimeHarp is the first truely continuous TCSPC system, ideally suited e.g. for time resolved single molecule detection. All functions of the system are controlled by a 32-bit Windows™ based software. A driver library (DLL) for 32-bit Windows™ program development is available to build custom applications.
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The TimeHarp can be used in different operation modes:
- Integration mode
- Oscilloscope mode
- Continuous mode f(t,T)
- Externally clocked continuous mode (scanning, imaging)
- Time-Tagged Time-Resolved mode (listing each event pair arrival time)*
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Current software and Developers' Library version: 3.0
This version features an improved data visualization and printing, running on all current 32 bit Windows™ platforms incl. XP. Full integration with Windows™ device management. Preservation of individual settings on multi-user platforms. Improved continuous mode data throughput on Windows™ NT and 2000. Owners of previous versions receive a free update. The Developers' library supports custom programming in C/C++, Delphi™, Visual Basic™ and LabVIEW™. Demo code is provided for an easy start.
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To learn more about Time-Correlated Single Photon Counting and the use of the TimeHarp 200, please request our Tech Note on TCSPC.
For further details about the Time-Tagged Time-Resolved mode (TTTR), please request our TechNote on the TTTR mode.
* available as an option
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Specifications: |
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Electrical Parameters |
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Operation and software features |
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Measurement Examples: |
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Endothelin receptor in virus-like particles: Ligand binding observed by fluorescence fluctuation spectroscopy, L. Zemanovál et al., University of Ulm |
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Fluorescence depolarization in the presence of excitation energy migration in partly ordered polymer films, A. Synak et al., University of Gdansk |
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Effect of protonation and hydrogen bonding on the fluorescent properties and exciplex formation of N-(4-pyridyl)-1,2-naphthalimide, K. Sebők-Nagy et al., Hungarian Academy of Science |
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List of all publications contained in our bibliography that are related to the TimeHarp 100/200 |
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Please see our bibliography for many other application examples |
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