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Fluorescence Lifetime Imaging and Correlation Software |
SymPhoTime |
Specifications
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Data Aquisition |
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TCSPC device |
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TimeHarp 200 TCSPC board and PicoHarp 300 and HydraHarp 400 TCSPC modules |
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Supported configurations |
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100 × 100 (× 100) µm Piezo Scanning Stage or 10 × 10 cm Wide Range Scanner Laser Scanning Microscopes (LSM), Stand-alone TCSPC |
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Routing |
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1 to 4 detectors |
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Measurement modes |
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TTTR: t3R, t2, t3 |
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Analysis |
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Supported methods |
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FLIM, FRET, Anisotropy, PIE, FCS, FCCS, FLCS, SMD, PCH Calculation, Lifetime Histogram,
Fluorescence Time/Lifetime Traces, BIFL, On / Off Histogram, Burst Size Histogram,
TCSPC Lifetime Fitting, FCS Fitting, User Scripting (STUPSLANG) |
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TCSPC Fitting |
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Methods |
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FLIM, fluorescence time traces, BIFL, lifetime histogramming |
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Models |
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1 to 4 exponentials, iterative reconvolution |
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Optimisation |
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Least squares, MLE, Marquardt-Levenberg, Monte Carlo |
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Error test / assessment |
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χ2, distribution weighted residuals |
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Error analysis |
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Asymptotic standard errors |
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FCS Fitting |
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Models |
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Pure diffusion, triplet-state, conformational, protonation 2D/3D Gauss. PSF |
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Optimisation |
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Least squares, Marquardt-Levenberg, Monte Carlo |
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Error test / assessment |
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χ2, distribution weighted residuals |
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Error analysis |
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Asymptotic standard errors, Bootstrap and support plane analysis |
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User interface |
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Graphical user interface |
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Windows GUI, menu or mouse driven |
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Preferences |
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Saved in windows registry |
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Data Formats |
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Supported formats |
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TimeHarp t3r, PicoHarp pt3, pt2, HydraHarp ht3, ht2 |
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Operating Environment |
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Required PC |
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minimum 400 MHz CPU clock, 4096 MB memory; recommended: dual-core; RAM >=512 MB |
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Display |
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1200 × 1024 or better |
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Disk space |
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20 MB (except data storage) |
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Operating system |
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Windows XP/Vista |
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Protection module port |
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USB (parallel on request) |
All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.
© PicoQuant GmbH, 1998 - 2009
last updated 19.01.2009
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