 |
|
Fluorescence Lifetime Imaging and Correlation Software |
SymPhoTime |
Specifications
|
Data Aquisition |
|
TCSPC device |
|
TimeHarp 200 TCSPC board and PicoHarp 300 TCSPC module |
|
Supported configurations |
|
100 × 100 (× 100) µm Piezo Scanning Stage or 10 × 10 cm Wide Range Scanner Laser Scanning Microscopes (LSM), Stand-alone TCSPC |
|
Routing |
|
1 to 4 detectors |
|
Measurement modes |
|
TTTR: t3R, t2, t3 |
|
|
Analysis |
|
Supported methods |
|
FLIM, FRET, Anisotropy, PIE, FCS, FCCS, FLCS, SMD, PCH, Lifetime Histogram, Fluorescence Time / Lifetime Traces, BIFL, On / Off Histogram, Burst Size Histogram, TCSPC Lifetime Fitting, FCS Fitting, User Scripting (STUPSLANG) |
|
|
TCSPC Fitting |
|
Methods |
|
FLIM, fluorescence time traces, BIFL, lifetime histogramming |
|
Models |
|
1 to 4 exponentials, iterative reconvolution |
|
Optimisation |
|
Least squares, MLE, Marquardt-Levenberg, Monte Carlo |
|
Error test / assessment |
|
χ2, distribution weighted residuals |
|
Error analysis |
|
Asymptotic standard errors |
|
|
FCS Fitting |
|
Models |
|
Pure diffusion, triplet-state, conformational, protonation 2D/3D Gauss. PSF |
|
Optimisation |
|
Least squares, Marquardt-Levenberg, Monte Carlo |
|
Error test / assessment |
|
χ2, distribution weighted residuals |
|
Error analysis |
|
Asymptotic standard errors, Bootstrap and support plane analysis |
|
|
User interface |
|
Graphical user interface |
|
Windows™ GUI, menu or mouse driven |
|
Preferences |
|
Saved in windows registry |
|
|
Data Formats |
|
Supported formats |
|
TimeHarp t3R, PicoHarp pt3, pt2 |
|
|
Operating Environment |
|
Required PC |
|
min. 400 MHz CPU clock, 4096 MB memory; recommended: dual-core; RAM >=512 MB |
|
Display |
|
1200 × 1024 or better |
|
Disk space |
|
20 MB (except data storage) |
|
Operating system |
|
Windows™ 2000/XP |
|
Protection module port |
|
USB (parallel on request) |
All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or ommisions. Specifications and external appearences are subject to change without notice.
© PicoQuant GmbH, 1998 - 2008
last updated 08.05.2008
|
