Fluorescence Lifetime Imaging and Correlation Software

SymPhoTime

Specifications

	Data Aquisition
	TCSPC device		TimeHarp 200 TCSPC board, PicoHarp 300 or HydraHarp 400 TCSPC units
	Supported configurations		80 × 80 (× 80) µm Piezo scanning stage or 8 × 8 cm wide range scanner Laser Scanning Microscopes (LSM), stand-alone TCSPC
	Routing		1 to 4 detectors
	Measurement modes		Time-Tagged Time-Resolved (TTTR) data acquisition
	Analysis
	Supported methods		FLIM, FRET, anisotropy, PIE, FCS, FCCS, FLCS, single molecule spectroscopy, PCH calculation, lifetime histogram, fluorescence time/lifetime traces, BIFL, on/off histogram, burst size histogram, TCSPC lifetime fitting, FCS fitting, user scripting ("STUPSLANG")
	TCSPC Fitting
	Methods		FLIM, fluorescence time traces, BIFL, lifetime histogramming
	Models		1 to 4 exponentials, iterative reconvolution
	Optimisation		least squares, MLE, Marquardt-Levenberg, Monte Carlo
	Error test/assessment		χ2, distribution weighted residuals
	Error analysis		Asymptotic Standard Errors (ASE)
	FCS Fitting
	Models		pure diffusion, triplet-state, conformational, protonation 2D/3D Gauss. PSF
	Optimisation		least squares, Marquardt-Levenberg, Monte Carlo
	Error test/assessment		χ2, distribution weighted residuals
	Error analysis		Asymptotic Standard Errors (ASE), bootstrap and support plane analysis
	User Interface
	Graphical user interface		Windows GUI, menu or mouse driven
	Preferences		Saved in windows registry
	Data Formats
	Supported formats		TimeHarp t3r, PicoHarp pt3, pt2, HydraHarp ht3, ht2
	Operating Environment
	Required PC		Dual-core processor with 2 GHz CPU clock (or better), RAM>= 1 GB
	Display		1200 × 1024 or better
	Operating system		Windows XP/Vista/7
	Protection module port		USB

All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.
© PicoQuant GmbH, 1998 - 2012