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Features |
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Easy connection of 4 detectors to one TimeHarp 200 board |
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Allowing simultaneous measurements on 4 channels |
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Deadtime < 70 ns |
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Easy to install in the signal path with SMA-connectors |
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Fully supported by TimeHarp 200 software |
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Support of Oscilloscope, Integration and TTTR measurement mode |
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Applications |
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Simultaneous recording of anisotropy or different spectral information |
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Multi-colour Single Molecule Spectroscopy (SMS) |
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Multi-colour Fluorescence Lifetime Imaging (FLIM) |
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Fluorescence Correlation Spectroscopy (FCS) of mixed fluorophores |
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Diffuse Optical Tomography (DOT) |
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A Brief Description |
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.jpg "TimeHarp 200 board with PRT 400 router") The PRT 400 is an accessory for the TimeHarp 200 TCSPC-board. It permits to operate up to 4 SPAD detectors with TTL output (e.g. Perkin-Elmer SPCM-AQR or PDM-Series) quasi in parallel on one TimeHarp 200 board, allowing users to collect fluorescence lifetime decays on multiple channels simultaneously. This permits simplified collection of e.g. polarization dependent data as well as sophisticated new multi-dimensional fluorescence detection methods in the life sciences or in general sensitive analytics, down to the single molecule level. The 4-channel router is powered by the TimeHarp 200 board and supports both histogramming and Time-tagged Time-resolved (TTTR) mode. The latter allows the recording of each individual photon with its picosecond timing, the arrival time at 100 ns resolution and the detector channel it came from. This permits ultimate flexibility in data analysis e.g. for single molecule burst detection or Fluorescence Correlation Spectroscopy (FCS) combined with fluorescence lifetime information. Using two detectors and TTTR mode it is possible to perform cross correlated FCS, thereby eliminating afterpulsing artefacts of the SPAD detectors.
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Specifications: |
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List of all publications contained in our bibliography that are related to the TimeHarp 200 |
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Please see our bibliography for many other application examples |
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