PCI-Board for Multichannel Scaling or Photon Counting

NanoHarp 250

Measurement Examples

Uranyl nitrate hexahydrate in water

Phosphorescence decay of 1 µM uranyl nitrate hexahydrate in water measured with the FluoTime 200 lifetime spectrometer and the NanoHarp250 board. The FluoTime was equipped with a Xenon flashlamp that provided 350 ns long flashes at 200 Hz repetition rate. 100 mm focal length monochromators were used to select the 280 nm excitation light as well as the 520 nm phosphorescence photons for detection with the PMA 182. The output of the detector was fed to the NanoHarp 250 board which was configured so that total 64 microsecond time interval was covered by 2048 channels with 32 ns channel width.
The plot shows the measured Instrument Response Function (red), the sample decay (blue) and the fitted decay. The recovered lifetime is 4.2 microseconds. Double exponential reconvolution analysis using FluoFit 4.0 reveals a presence of a small amount of faster decay component with 500 ns lifetime (2% fractional intensity). An excellent chi-square of 0.952 and the residual trace demonstrate the quality of the fit.

Tris(2,2´-bipyridyl) rhutenium(II) chloride in water

The figure below shows the results of a 10 second measurement with the NanoHarp 250. Here we excited the sample, Tris(2,2´-bipyridyl) rhutenium(II) chloride in distilled water, 1 µM concentration, with LDH-P-C-440 laser diode head driven by PDL800-B, externally triggered at 200 kHz. Luminescence was detected by a PMA 185 detector through a ScienceTech 9030 monochromator set to 620 nm with a 32 nm bandpass. In this case reconvolution fitting is not necessary, as the IRF is far too small to have an influence on the measured decay. Instead tailfitting was applied, which yielded a lifetime of 372 ns. An excellent chi-square of 0.984 and the residual trace demonstrate the quality of the fit.

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last updated 02.05.2005