 |
|
Inverse Time-resolved Fluorescence Microscope |
MicroTime 200 |
Specifications
| Excitation (optional) |
| Light source |
|
Picosecond Laser Diode Heads (LDH Series) |
| Wavelengths |
|
375 - 510 nm, 530 nm, 635 - 900 nm |
| Repetition rate |
|
up to 40 MHz
(80 MHz optional) |
|
| Scanning (optional) by Physik Instrumente |
| Controller |
|
E-710 digital PZT controller with special PicoQuant modification |
| 2D scanning stage |
|
P-733 piezo-positioner |
| Range / resolution |
|
80 µm x 80 µm range with less than 10 nm resolution |
| Z scanning |
|
P-721 PIFOC objective nano-positioner |
| Range / resolution |
|
80 µm range with less than 10 nm resolution |
|
| Objectives |
| Standard |
|
PL 20x3 PlanAchromat, NA 0.4, air spaced, 400 - 750 nm |
|
|
PL 40x PlanAchromat, NA 0.65, air spaced, 400 - 750 nm |
|
|
UPLSAPO 60x PlanApochromat, NA 1.2, water immersion, 400 - 900 nm |
| Optional |
|
oil immersion, apochromatic correction, air spaced, IR/UV-enhanced or long working distance,TRIF objectives |
|
| Detectors |
| Type |
|
τ-SPAD |
SPAD (PDM Series) |
| Spectral range |
|
400 - 1000 nm |
400 - 1000 nm |
| Dark counts (at 20°C, typ. value) |
|
< 100 cps |
< 250 cps |
|
| Data Acquisition |
| Type |
|
PicoHarp 300 |
|
HydraHarp 400 |
| Time resolution (bin width) |
|
4 ps |
|
1 ps |
| Dead time |
|
< 95 ns |
|
< 80 ns |
Discriminator level
zero cross adjust |
|
software adjustable |
| Collection time |
|
1 ms to 10 h |
|
| Software features |
| General concept |
|
use of versatile TTTR file format for data acquisition, data archiving in workspace, time gating for all methods, separation of up to four detector signals, scripting language for user-definded analysis procedures |
| Point measurements |
|
data conversion to: MCS trace, F(C)CS calculation and fitting, FLCS, TCSPC histogram, on/off-state histogram, burst size analysis, (PIE-)FRET histogram, photon counting histogram, lifetime histogram, antibunching plot |
Fluorescence Lifetime Imaging
(FLIM) |
|
data conversion to: fluorescence intensity images, fluorescence lifetime images, time gated analysis, TCSPC histogram for region of interest, decay analysis |
|
| Operational & Electrical |
| Operating environment |
|
Quad-core CPU > 3 GHz, RAM >= 4 GB |
| Power requirements |
|
110/230 V, 50/60 Hz |
|
| Dimensions |
| Laser combining unit |
|
600 mm x 400 mm x 200 mm (without laser driver) |
| Microscope and main optical unit |
|
1150 mm x 600 mm (2 detection channels) |
| 19'' electronic rack (typical) |
|
500 mm x 550 mm x 400 mm |
| Table |
|
Maximum spacing between each unit is 1.5 m. It is recommended to allow 0.5 m
access around all sides of the system. Minimum required space on optical table for
microscope and main optical unit: 1150 × 600. |
|
|
Breadboard optionally available from PicoQuant:
1600 × 600 × 50, M6 holes, 25 mm grid
4 ft × 2ft
× 2 inch, ¼-20 holes, 1 inch grid. |
All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.
© PicoQuant GmbH, 1998 - 2012
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