 |
|
Inverse Time-resolved Fluorescence Microscope |
MicroTime 200 |
Specifications
| Excitation (optional) |
| Light source |
|
Picosecond Diode Lasers |
| Wavelengths |
|
375 - 485 nm, 530 nm, 635 - 900 nm |
| Repetition rate |
|
Up to 40 MHz
(80 MHz optional) |
|
| Scanning (optional) by Physik Instrumente |
| Controller |
|
E-710 digital PZT controller with special PicoQuant modification |
| 2D scanning stage |
|
P-733 piezo-positioner |
| Range / resolution |
|
100 µm x 100 µm range with less than 10 nm resolution |
| Z scanning |
|
P-721 PIFOC objective nano-positioner |
| Range / resolution |
|
100 µm range with less than 10 nm resolution |
|
| Objectives |
| Standard |
|
PL 20x3 PlanAchromat, NA 0.4, air spaced, 400 - 750 nm |
|
|
PL 40x PlanAchromat, NA 0.65, air spaced, 400 - 750 nm |
|
|
UPLFL 100x PlanFluorit, NA 1.3, oil immersion, 350 - 800 nm |
| Optional |
|
Water or oil immersion, apochromatic correction, air spaced, IR/UV-enhanced or long working distance objectives |
|
| Data Acquisition |
| Type |
|
PicoHarp 300 |
| Time resolution (bin width) |
|
4 ps |
| Dead time |
|
< 90 ns |
| Discriminator level, zero cross adjust |
|
Software adjustable |
| Collection time |
|
1 ms to 10 h |
|
| Software features |
| General concept |
|
Use of versatile TTTR file format for data acquisition, data archiving in workspace, time gating for all methods, separation of up to four detector signals |
| Point measurements |
|
Data conversion to: MCS trace, FCS calculation and fitting, TCSPC histogram, on/off-state histogram, burst size analysis, (PIE-)FRET histogram, Photon counting histogram, Lifetime histogram |
Fluorescence Lifetime Imaging
(FLIM) |
|
Data conversion to: fluorescence intensity images, fluorescence lifetime images, time gated analysis, TCSPC histogram for region of interest |
|
| Operational & Electrical |
| Computer system |
|
PC Pentium class, 2 GHz, 1024 MB memory with Windows® XP |
| Power requirements |
|
110/230 V 50/60 Hz |
|
| Dimensions |
| Laser coupling unit |
|
400 mm x 320 mm x 140 mm (without laser driver) |
| 19'' electronic rack (typical) |
|
500 mm x 550 mm x 400 mm |
| Table |
|
Mamimum spacing between each unit is 1.5 m. It is recommended to allow 0.5 m
access around all sides of the system. Minimum required space on optical table for
microscope and main optical unit: 1100 × 600. |
|
|
Breadboard optionally available from PicoQuant:
1200 × 600 × 50, M6 holes, 25 mm grid
4 ft × 2ft
× 2 inch, ¼-20 holes, 1 inch grid. |
All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or ommisions. Specifications and external appearences are subject to change without notice.
© PicoQuant GmbH, 1998 - 2008
last updated 18.09.2008
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