 |
|
Upright Time-resolved Fluorescence Microscope  |
MicroTime 100 |
Specifications
| Scanning (optional) |
| Type |
|
High precision |
|
Large area |
| Range |
|
100 µm × 100 µm |
|
10 cm × 10 cm |
| Positioning accuracy |
|
<10 nm |
|
<10 µm |
|
| Objectives |
| Standard |
|
PL 20x3 PlanAchromat, NA 0.4, air spaced, 400 - 750 nm |
|
|
PL 40x PlanAchromat, NA 0.65, air spaced, 400 - 750 nm |
|
|
UPLFL 100x PlanFluorit, NA 1.3, oil immersion, 350 - 800 nm |
| Optional |
|
Water or oil immersion, apochromatic correction, air spaced, IR/UV-enhanced or long working distance objectives |
|
| Detectors |
| Type |
|
PMA Photomultiplier assembly |
PDM SPAD |
| Spectral range |
|
185 - 650 nm |
185 - 820 nm |
400 - 1000 nm |
| Dark counts (at 25°C) |
|
< 100 cps |
< 1000 cps |
< 250 cps |
| Lifetime resolution down to |
|
50 ps |
20 ps |
|
| Data Acquisition |
| Type |
|
PicoHarp 300 |
| Time resolution (bin width) |
|
4 ps |
| Dead time |
|
< 95 ns |
| Discriminator level, zero cross adjust |
|
Software adjustable |
| Collection time |
|
1 ms to 10 h |
|
| Software features |
| General concept |
|
Use of versatile TTTR file format for data acquisition, data archiving in workspace, time gating for all methods, separation of up to four detector signals |
| Point measurements |
|
Data conversion to: MCS trace, FCS calculation and fitting, TCSPC histogram, on/off-state histogram, burst size analysis, (PIE-)FRET histogram, Photon counting histogram, Lifetime histogram |
Fluorescence Lifetime Imaging
(FLIM) |
|
Data conversion to: fluorescence intensity images, fluorescence lifetime images, time gated analysis, TCSPC histogram for region of interest |
|
| Operational & Electrical |
| Computer system |
|
PC Pentium class, 2 GHz, 1024 MB memory with Windows® XP |
| Power requirements |
|
110/230 V 50/60 Hz |
|
| Dimensions |
| Microscope unit |
|
320 × 600 × 600 mm (w × d × h) |
All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or ommisions. Specifications and external appearences are subject to change without notice.
© PicoQuant GmbH, 1998 - 2008
last updated 18.09.2008
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