 |
|
Upright Time-resolved Fluorescence Microscope |
MicroTime 100 |
Specifications
| Scanning (optional) |
| Type |
|
High precision |
|
wide-range |
| Range |
|
80 µm × 80 µm |
|
8 cm × 8 cm |
| Positioning accuracy |
|
<10 nm |
|
<10 µm |
|
| Objectives |
| Standard |
|
PL 20x3 PlanAchromat, NA 0.4, air spaced, 400 - 750 nm |
|
|
PL 40x PlanAchromat, NA 0.65, air spaced, 400 - 750 nm |
|
|
UPLFL 100x PlanFluorit, NA 1.3, oil immersion, 350 - 800 nm |
| Optional |
|
Water or oil immersion, apochromatic correction, air spaced, IR/UV-enhanced or long working distance objectives |
|
| Detectors |
| Type |
|
PMT (PMA Series) |
SPAD (PDM Series) |
| Spectral range* |
|
185 - 650 nm |
185 - 820 nm |
400 - 1000 nm |
| Dark counts (at 20°C, typ. value) |
|
< 50 cps |
< 900 cps |
< 250 cps |
| Lifetime resolution down to |
|
50 ps |
20 ps |
|
| Data Acquisition |
| Type |
|
PicoHarp 300 |
| Time resolution (bin width) |
|
4 ps |
| Dead time |
|
< 95 ns |
| Discriminator level, zero cross adjust |
|
Software adjustable |
| Collection time |
|
1 ms to 10 h |
|
| Software features |
| General concept |
|
Use of versatile TTTR file format for data acquisition, data archiving in workspace, time gating for all methods, separation of up to four detector signals |
| Point measurements |
|
Data conversion to: MCS trace, FCS calculation and fitting, TCSPC histogram, on/off-state histogram, burst size analysis, (PIE-)FRET histogram, Photon counting histogram, Lifetime histogram |
Fluorescence Lifetime Imaging
(FLIM) |
|
Data conversion to: fluorescence intensity images, fluorescence lifetime images, time gated analysis, TCSPC histogram for region of interest |
|
| Operational & Electrical |
| Computer system |
|
PC Pentium class, 2 GHz, 1024 MB memory with Windows® XP/Vista/7 |
| Power requirements |
|
110/230 V 50/60 Hz |
|
| Dimensions |
| Microscope unit |
|
320 × 600 × 600 mm (w × d × h) |
* other types and cooling available on request
All Information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice.
© PicoQuant GmbH, 1998 - 2010
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