Fluorescence Lifetime Spectrometer

FluoTime 200

FluoTime 200 - L-Tryptophane dissolved in water 

A solution of L-Tryptophane in water (HPLC quality) was excited with the PLS 300 at 1.8 µW average power, driven by the PDL 800-B driver at 10 MHz repetition rate. The collected emission passed a monochromator set to 350 nm with 4 nm bandpass. The pulses from PMA-182 detector were processed by the TimeHarp 200 TCSPC board. The measured instrument response function (IRF) has a FWHM of 600 ps and 1.5 million counts were collected within 50 seconds measurement time. No polarizers were used in the setup.

The measurement results were analysed using FluoFit - as can be expected for L-Tryptophane the decay is best described by a triple exponential decay funciton. The picture below shows the IRF (red), the measured decay (blue) and the result of the fit (black). The recovered lifetimes are 0.35 ± 0.18 ns , 2.65 ± 0.1 ns and 7,93 ± 0.1 ns. The errors were calculated using the support plane analysis.