TRES Analysis software

FluoPlot

FluoPlot - a software to analyse Time-Resolved Emission Spectra

In time-resolved fluorescence research, it is often of great interest to observe time-dependent spectral shifts and decay changes in the context of solvent relaxation dynamics and general spectral evolution. This requires recording Time-Resolved Emission Spectra (TRES), ideally with automated wavelength scan and TCSPC data collection under full software control.

In order to measure emission spectra in combination with fluorescence lifetime, the FluoTime 200 data acquisition software (either from TimeHarp 200, NanoHarp 250 , PicoHarp 300 or from HydraHarp 400 ) provides an automated TRES measurement mode. This mode allows to control a monochromator via a stepper motor and automated collection of spectrally resolved lifetime histograms.

For the analysis of these measurements, the Fluoplot software is provided with the system. It is designed to support your work in respect of analysis, illustration and documentation and in providing many useful graphics and data import and export facilities. It also interfaces with the fluorescence decay analysis package FluoFit. Intuitive navigation and convenient control by mouse or keyboard will let you feel familiar after a short time of customization.

The FluoPlot software includes many useful functions and visualisation tools, such as

• 3D visualisation of the measured decay with free 3D rotation capabilities, adjustable color scale, different projection styles and various export functions
• 2D visualisation of the measured decays
• Adjustable 2D cuts through the 3D visualisation for
  • Emission spectrum as a function of decay time
  • Fluorescence decay as a function of emission wavelength
• Interval analysis of TRES measurements: combining multiple time or wavelength channels to achieve higher statistics; definition and display of multiple intervals
• Spectral correction of wavelength data
• Export of all data to ASCII