FluoFit Version 4.2.9 now supports global fitting, batch fitting and anisotropy reconvolution analysis as well as maximum-likelihood estimation !

The FluoFit software package is a powerful analysis software for fluorescence decay and anisotropy measurements. Tail fitting as well as a numerical reconvolution algorithm to account for the finite Instrument Response Function (IRF) can be applied. The decay data can be fitted to exponential decay models up to 4th order or alternatively to different lifetime distribution models (Gaussian, Lorentzian and Stretched Exponential). Global fitting as well as batch mode fitting is supported for all models. The software allows to freely vary the number of fit parameters, including IRF and signal background as well as time shift. Start parameters for the fitting algorithm can be determined automatically or entered manually. The fitting limits are easily adjusted with graphical sliders. Reduced chi-square, weighted residual and autocorrelation trace are shown for assessment of the goodness of fit. An advanced error analysis using different methods is also possible. User preferences are widely adjustable and can be automatically stored and retrieved. The 32-bit software is available for Windows ME/2000/XP and features a modern and easy to use graphical user interface. Results can be printed, saved and exported for later reference. The program supports data files from the HydraHarp 400, PicoHarp 300, TimeHarp 200, NanoHarp 250, SPC and MSA TCSPC systems as well as ASCII data files. Instrument response and fluorescence decay may be loaded from different sources. A comprehensive on-line help library is provided for ease of use.

The FluoFit software is available in two versions to meet different needs. The main features of both versions (Basic and Professional) are shown in the following table:

	FluoFit Basic	FluoFit Professional
Data import from HydraHarp, PicoHarp, TimeHarp, NanoHarp, SPC, MSA, ASCII
Exponential decay models
Lifetime distributions models
Anisotropy analysis
Global analysis and batch fitting
Maximum likelihood estimation
Goodness of fit assessment
Error analysis
Export of analysis results
Storage of user preferences

Current software version: 4.2.9

Please note:

• The new functionalities of version 4.2.9 are only supported by FluoFit Professional
• Users of older versions may upgrade for free to the functionalities of FluoFit Basic
• For an upgrade to the current FluoFit version, please contact PicoQuant or your local distributor

To test the functionalities of the software, please request an evaluation package or send us your data for test fitting.