2nd European Short Course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" November 1-5, 2004.
with kind support of
Center for Fluorescence Spectroscopy &
Department of Biochemistry and Molecular Biology
University of Maryland Medical School
Baltimore, Maryland 21201
Course Chairman: Rainer Erdmann, PicoQuant GmbH
Summary
This course was held in Europe for the second time and was a great success. Altogether 73 people from universities and industry from more than 15 countries participated. They attended 18 lectures and 3 hands-on experiment sessions with 10 different instruments from 5 companies, covering different methods and procedures in fluorescence spectroscopy.
  
Aims and Purpose
The course was intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees were typically professionals who are using or intend to use fluorescence in their research. Most attendees had some knowledge of fluorescence, typically in a specialized area. However, other individuals from totally different research areas and industry, got the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.
Course Instructors:
Joseph R. Lakowicz (Baltimore), Richard Thompson (Baltimore), Zygmunt "Karol" Gryczynski (Baltimore), Rainer Erdmann (Berlin), Michael Wahl (Berlin), Matthias Patting (Berlin)
Guest Lecturers:
Manfred Auer (Novartis AG) High Throughput Screening
Sabato D'Auria (Uni Naples) Structure, stability, conformational dynamics and biotechnological applications of thermophilic enzymes
Joerg Enderlein (FZ Juelich) Fluorescence Fluctuation Spectroscopy
Stefan Hell (MPI Goettingen) High Resolution Microscopy
Otto S. Wolfbeis (Uni Regensburg) Markers, Probes and Labels
Course Organization
The course was offered in two sections: Part I was primarily basic principles, and Part II went into details and stressed applications.
I. Principles of Fluorescence Spectroscopy
Part I covered the basics of steady state and time-resolved fluorescence spectroscopy and instrumentation, and was designed for individuals who are not experts. Section I covered time- and frequency-domain measurements, anisotropy, solvent effects, quenching and Foerster energy transfer, as well as an introduction into data analysis.
II. Time-Resolved Fluorescence Spectroscopy, Principles, Instrumentation and Applications
Part II emphasised time-resolved fluorescence, together with advanced applications of fluorescence in biophysics, sensing, imaging, clinical chemistry, multi-photon excitation and ultrasensitive detection. Part II included guest lectures on fluorescence microscopy, multi-photon microscopy, correlation spectroscopy, single molecule detection and fluorescence markers as well as high-throughput screening.
Parts I and II consisted of lectures and a certain portion of hands-on experimentation (steady state and time-resolved experiments).
Hands-on instrumentation
The following companies offered hands-on experiments during the course:
- Varian
- HORIBA Jobin Yvon GmbH
- Olympus
- PTI
- PicoQuant
Program / Location
The program and the time schedule are available for download in PDF format:
Special public lecture
Prof. J.R. Lakowicz & Prof. Z. Gryczynski
Center for Fluorescence Spectroscopy (CFS) / University of Maryland, USA
1. Radiative Decay Engineering: The Use of Metallic Nano-Structures to Control Emission Properties of Fluorophores
2. Surface Plasmon-Coupled Emission: Ultra Sensitive Fluorescence Detection Technology
The special lecture took place on Wednesday, 3 November 2004, 1:30 p.m. at the Max Born Saal, WISTA Campus, 12489 Berlin-Adlershof, Carl-Scheele-Str. 6 / Max-Born-Str.
About the Instructors and Guest Lecturers
The course was taught together by Dr. Joseph R. Lakowicz and staff at the CFS (Baltimore) as well as Rainer Erdmann and staff of PicoQuant GmbH (Berlin), plus guest lecturers. These individuals all have extensive experience in time-resolved fluorescence and its applications to Life Sciences.
Joseph R. Lakowicz cfs.umbi.umd.edu/jrl
is Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy.
Richard Thompson
is Assistant Professor of Biochemistry and specializes in fiber-optic sensing and medical diagnostics.
Rainer Erdmann www.picoquant.com
is Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
Michael Wahl www.picoquant.com
is Senior Scientist at PicoQuant GmbH and specializes in instrumentation for time-correlated single photon counting instrumentation
Joerg Enderlein www.joerg-enderlein.de
is Head of the Single Molecule Detection group at FZ Juelich and specializes in Single Molecule Detection, fluorescence correlation spectroscopy and complex data analysis
Stefan Hell www.4pi.de
is Director of the High Resolution Optical Microscopy Group at Max Planck Institute for Biophysical Chemistry, Göttingen, Germany and specializes in new methods for microscopy with improved resolution and other innovative forms of biological imaging.
Manfred Auer
is Head of Innovative Screening Technologies (IST) at the Novartis Lead Discovery Center, as well as Univ. Dozent for Biophysical Chemistry at the University of Salzburg, Austria. He specializes in the development of novel drug discovery technologies.
Otto S. Wolfbeis www.wolfbeis.de
is Professor and Vice Rector/President at Regensburg university and specializes in design and synthesis of long-wavelength and long-lifetime fluorescent bioprobes and on fluorescence based optical sensors
Zygmunt "Karol" Gryczynski www.hsc.unt.edu/departments/molbioim/biography.cfm?id=472
Professor at the Department of Molecular Biology and Immunology at the University of North Texas
Mathias Patting www.picoquant.com
is Senior Scientist at PicoQuant GmbH and specializes in software design for time-correlated single photon counting instrumentation
Sabato (Tino) D'Auria
is Senior Scientist at the Institute of Protein Biochemistry, Italian National Research Council, Naples, Italy.
Previous Courses
The European short course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.
Previous Courses
The European short course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.
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