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1st European Short Course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" October 20-24, 2003.
Course Chairman: Rainer Erdmann, PicoQuant GmbH SummaryThis course was held in Europe for the first time and was a great success. Altogether 86 people from universities and industry from more than 15 countries participated. They attended 17 lectures and 3 hands-on experiment sessions with 10 different instruments covering different methods and procedures in fluorescence spectroscopy.
Aims and PurposeThe course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, also individuals from totally different areas as well as from industry get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.Course InstructorsJoseph R. Lakowicz (Baltimore), Richard Thompson (Baltimore), Rainer Erdmann, (Berlin), Michael Wahl (Berlin) Manfred Auer (Novartis AG) High Throughput Screening Joerg Enderlein (FZ Juelich) Fluorescence Fluctuation Spectroscopy Stefan Hell (MPI Goettingen) High Resolution Microscopy Otto S. Wolfbeis (Uni Regensburg) Markers, Probes and Lables Course OrganizationThe course was offered in two sections. Part I was primarily basic principles, and Part II went into details and stressed applications. I. Principles of Fluorescence Spectroscopy Part I covered the basics of steady state and time-resolved fluorescence spectroscopy and instrumentation, and was designed for individuals who are not experts. Section I covered time- and frequency-domain measurements, anisotropy, solvent effects, quenching and Foerster energy transfer, as well as an introduction into data analysis. II. Time-Resolved Fluorescence Spectroscopy, Principles, Instrumentation and Applications Part II emphasised time-resolved fluorescence, together with advanced applications of fluorescence in biophysics, sensing, imaging, clinical chemistry, multi-photon excitation and ultrasensitive detection. Part II included guest lectures on fluorescence microscopy, multi-photon microscopy, correlation spetroscopy, single molecule detection and fluorescence markers as well as high-thoughput screening. Parts I and II consisted of lectures and a certain portion of hands-on experimentation (steady state and time-resolved experiments) Hands-on instrumentation
The following companies offered hands-on experiments during the course:
The course was divided in 17 lectures and 3 afternoon hands-on sessions. The program and the time schedule are available for download in PDF format. About the InstructorsThe course was taught together by Dr. Joseph R. Lakowicz and staff at the CFS (Baltimore) as well as Rainer Erdmann and staff of PicoQuant GmbH (Berlin), plus guest lecturers. These individuals all have extensive experience in time-resolved fluorescence and its applications to Life Sciences.Joseph R. Lakowicz cfs.umbi.umd.edu/jrl is Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy. Richard Thompson is Assistant Professor of Biochemistry and specializes in fiber-optic sensing and medical diagnostics. Rainer Erdmann www.picoquant.com is Managing Director of the R/D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis Michael Wahl www.picoquant.com is Senior Scientist at PicoQuant GmbH and specializes in instrumentation for time-correlated single photon counting instrumentation Joerg Enderlein www.joerg-enderlein.de is Head of the Single Molecule Detection group at FZ Juelich and specializes in Single Molecule Detection, fluorescence correlation spectroscopy and complex data analysis Stefan Hell www.4pi.de is Director of the High Resolution Optical Microscopy Group at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany and specializes in new methods for microscopy with improved resolution and other innovative forms of biological imaging. Manfred Auer is Head of Innovative Screening Technologies (IST) at the Novartis Lead Discovery Center, as well as Univ. Dozent for Biophysical Chemistry at the University of Salzburg, Austria. He specializes in the development of novel drug discovery technologies. Otto S. Wolfbeis www.wolfbeis.de is Professor and Vice Rector/President at Regensburg university and specializes in design and synthesis of long-wavelength and long-lifetime fluorescent bioprobes and on fluorescence based optical sensors Zygmunt "Karol" Gryczynski www.hsc.unt.edu/departments/molbioim/biography.cfm?id=472 Professor at the Department of Molecular Biology and Immunology at the University of North Texas Mathias Patting www.picoquant.com is Senior Scientist at PicoQuant GmbH and specializes in software design for time-correlated single photon counting instrumentation Further CoursesThe European short course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below. Previous CoursesThe European short course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.
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