1st European Short Course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" October 20-24, 2003.

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Aims and Purpose Course Instructors Guest Lecturers Course Organization
Program Hands-on Instrumentation About the Instructors Information and Correspondence
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with kind support of
Center for Fluorescence Spectroscopy &
Department of Biochemistry and Molecular Biology
University of Maryland Medical School
Baltimore, Maryland 21201

Course Chairman: Rainer Erdmann, PicoQuant GmbH


Summary

This course was held in Europe for the first time and was a great success. Altogether 86 people from universities and industrie from more than 15 countries participated. They attended 17 lectures and 3 hands-on experiment sessions with 10 different instruments covering different methods and procedures in fluorescence spectroscopy.


Aims and Purpose

The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, also individuals from totally different areas as well as from industry get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.


Course Instructors

Joseph R. Lakowicz (Baltimore), Richard Thompson (Baltimore), Rainer Erdmann, (Berlin), Michael Wahl (Berlin)


Guest Lecturers*:

Manfred Auer (Novartis AG) High Throughput Screening

Joerg Enderlein (FZ Juelich) Fluorescence Fluctuation Spectroscopy

Stefan Hell (MPI Goettingen) High Resolution Microscopy

Otto S. Wolfbeis (Uni Regensburg) Markers, Probes and Lables


Course Organization

The course was offered in two sections. Part I was primarily basic principles, and Part II went into details and stressed applications.

I. Principles of Fluorescence Spectroscopy

Part I covered the basics of steady state and time-resolved fluorescence spectroscopy and instrumentation, and was designed for individuals who are not experts. Section I covered time- and frequency-domain measurements, anisotropy, solvent effects, quenching and Foerster energy transfer, as well as an introduction into data analysis.

II. Time-Resolved Fluorescence Spectroscopy, Principles, Instrumentation and Applications

Part II emphasised time-resolved fluorescence, together with advanced applications of fluorescence in biophysics, sensing, imaging, clinical chemistry, multi-photon excitation and ultrasensitive detection. Part II included guest lectures on fluorescence microscopy, multi-photon microscopy, correlation spetroscopy, single molecule detection and fluorescence markers as well as high-thoughput screening.

Parts I and II consisted of lectures and a certain portion of hands-on experimentation (steady state and time-resolved experiments)


Hands-on instrumentation

The following companies offered hands-on experiments during the course:

  • Varian
  • Spex/JY/Horiba
  • Olympus
  • Tecan
  • PicoQuant


Program

The course was divided in 17 lectures and 3 afternoon hands-on sessions. The program and the time schedule are available for download in PDF format.


About the Instructors

The course was taught together by Dr. Joseph R. Lakowicz and staff at the CFS (Baltimore) as well as Rainer Erdmann and staff of PicoQuant GmbH (Berlin), plus guest lecturers. These individuals all have extensive experience in time-resolved fluorescence and its applications to Life Sciences.

Joseph R. Lakowicz cfs.umbi.umd.edu/jrl

is Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy.

Richard Thompson

is Assistant Professor of Biochemistry and specializes in fiber-optic sensing and medical diagnostics.

Rainer Erdmann www.picoquant.com

is Managing Director of the R/D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis

Michael Wahl www.picoquant.com

is Senior Scientist at PicoQuant GmbH and specializes in instrumentation for time-correlated single photon counting instrumentation

Joerg Enderlein www.joerg-enderlein.de

is Head of the Single Molecule Detection group at FZ Juelich and specializes in Single Molecule Detection, fluorescence correlation spectroscopy and complex data analysis

Stefan Hell www.4pi.de

is Director of the High Resolution Optical Microscopy Group at Max Planck Institute for Biophysical Chemistry, Gottingen, Germany and specializes in new methods for microscopy with improved resolution and other innovative forms of biological imaging.

Manfred Auer

is Head of Innovative Screening Technologies (IST) at the Novartis Lead Discovery Center, as well as Univ. Dozent for Biophysical Chemistry at the University of Salzburg, Austria. He specializes in the development of novel drug discovery technologies.

Otto S. Wolfbeis www.wolfbeis.de

is Professor and Vice Rector/President at Regensburg university and specializes in design and synthesis of long-wavelength and long-lifetime fluorescent bioprobes and on fluorescence based optical sensors

Zygmunt "Karol" Gryczynski www.hsc.unt.edu/departments/molbioim/biography.cfm?id=472

Professor at the Department of Molecular Biology and Immunology at the University of North Texas

Mathias Patting www.picoquant.com

is Senior Scientist at PicoQuant GmbH and specializes in software design for time-correlated single photon counting instrumentation


Previous Courses

The European short course on "Principles and Applications of Time-Resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.

  2003     2004     2005     2006     2007     2008  


Information and Correspondence

Course Coordinators: Angelika Zoufahl / Jana Gruenig

PicoQuant GmbH
Rudower Chaussee 29 (IGZ)
12489 Berlin, Germany

Phone: +49-30-63926560, Fax +49-30-63926561

e-mail: trfcoursepicoquant.com

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Rudower Chaussee 29 (IGZ)
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Germany
Phone:  ++4930 6392 6560
Fax: ++4930 6392 6561
Email: trfcoursepicoquant.com
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