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Science in your labs - Vienna 2014

PicoQuant: Science in your labs
“3rd FLIM and FCS Workshop”

November 18-19, 2014 in Vienna, Austria


Joint workshop of Max F. Perutz Laboratories, Campus Science Support Facilities (CSF) GmbH, and PicoQuant GmbH

Image Science in your labs - Vienna 2014 2014

Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) are particularly well suited to monitor interactions between molecules both in cells and in vitro. Yet, the 'activation energy' to apply these methods in life science research is relatively high. That is why one of the leading manufacturer of advanced imaging instruments PicoQuant, Vienna BioCenter Campus Science Support Facilities GmbH (CSF) and Max F. Perutz Laboratories (MFPL), which is a joint venture of the University of Vienna and Medical University of Vienna, joined efforts to organize the 3rd FLIM & FCS Workshop.

The workshop showcased the utility of the advanced imaging techniques in biological research and provide hands-on practicals using real samples in order to lower the 'activation barrier' for the potential users. Leading scientists introduced the concepts and applications of time-resolved fluorescence microscopy, fluorescence auto- and cross-correlation and anisotropy measurements, super-resolution imaging. Examples of their applications in biological research were demonstrated in the afternoon. Practical sessions also covered various aspects of sample preparation, data acquisition, and analysis. The program was completed by a poster session.

Speakers

  • Volker Buschmann (PicoQuant GmbH, Germany)
    “With Photon Patterns Towards Species Selective Microscopy”
  • Paul French (Imperial College London, United Kingdom)
    “Fluorescence Lifetime Imaging Across the Scales”
  • Thomas Ganz (FEMTOLASERS, Austria)
    “Multi-model Imaging Using a Noval and Portable Ultrafast Light Source”
  • Erwin Gaubitzer (MFPL, Austria)
    “A Short History of Fluorescence Fluctuations”
  • Enrico Gratton (University of California, Irvine, USA)
    “The iMSD method for imaging FCS”
  • Mark Hink (University of Amsterdam, The Netherlands)
    “Analysing noise: Quantitative cell biology by using Fluorescence Fluctuation Spectroscopy”
  • Jörg Langowski (DKFZ, Germany)
    “Live Maps of Protein Mobility by Light Sheet FCS”
  • Gregor Klatt (Laser Quantum, Germany)
    “Laser solutions for life science applications ”
  • Bernd Müller-Zülow (LaVision BioTec, Germany)
    “A fully integrated multi-photon based FLIM-System ”
  • Ulrich Nienhaus (Karslruhe Institute of Technology, Germany)
    “Novel Fluctucation Correlation Spectroscopy Techniques for a Wide Range of Applications”
  • Sandra Orthaus-Müller (PicoQuant GmbH, Germany)
    “Advanced Fluorescence Lifetime Imaging Microscopy (FLIM): Concepts and Applications”
  • Ivan Yudushkin (Max F. Perutz Laboratories, Austria)
    “Design of FRET probes”

Time schedule

Venue: IMP Main Lecture Hall, Dr. Bohr-Gasse 7, 1030 Vienna, Austria

The time schedule of the course is also available for download.

Tuesday, November 18

08:00 - 09:00 Registration
09:00 - 9:15 Welcoming address
Ivan Yudushkin and the Scientific Director (MFPL)
Kareem Elsayad (CSF)
9:15 - 9:45 The iMSD method for imaging FCS
Enrico Gratton (University of California)
9:45 - 10:45 Analysing noise: Quantitative cell biology by using Fluorescence Fluctuation Spectroscopy
Mark Hink (University of Amsterdam)
10:45 - 11:30 Coffee break and poster presentation
11:30 - 12:00 A Short History of Fluorescence Fluctuations
Erwin Gaubitzer (MFPL)
12:00 - 12:30 With Photon Patterns Towards Species Selective Microscopy
Volker Buschmann (PicoQuant GmbH)
12:30 - 12:45 Multi-model Imaging Using a Noval and Portable Ultrafast Light Source
Thomas Ganz (Femtolaser)
12:45 - 13:00 Group picture
13:00 - 14:00 Lunch break
14:00 - 16:00 Practical sessions (by appointment)
  • MicroTime 200 (FLIM/FCS/Anisotropy)
  • DeltaVision OMX (3D structured illumination)
  • Light-sheet
  • Brillouin scattering confocal microscopy
  • Choice of fluorophores and sample preparation
16:00 - 16:30 Coffee break and poster presentation
16:30 - 18:30 Practical sessions (by appointment)
  • MicroTime 200 (FLIM/FCS/Anisotropy)
  • DeltaVision OMX (3D structured illumination)
  • Light-sheet
  • Brillouin scattering confocal microscopy
  • Choice of fluorophores and sample preparation

Wednesday, November 19

9:00 - 9:30 Fluorescence Lifetime Imaging Across the Scales
Paul French (Imperial College London)
9:30 - 10:00 Novel Fluctuation Correlation Spectrosocopy Techniques for a Wide Range of Applications
Ulrich Nienhaus (Karlsruhe Institute of Technology)
10:00 - 10:30 Live Maps of Protein Mobility by Light Sheet FCS
Jörg Langowski (DKFZ)
10:30 - 11:15 Coffee break and poster presentation
11:15 - 11:30 Laser solutions for life science applications
Gregor Klatt (Laser Quantum)
11:30 - 12:00 Advanced Fluorescence Lifetime Imaging Microscopy (FLIM): Concepts and Applications
Sandra Orthaus-Müller (PicoQuant GmbH)
12:00 - 12:30 Design of FRET probes
Ivan Yudushkin (MFPL)
12:30 - 12:45 A fully integrated multi-photon based FLIM-System
Bernd Müller-Zülow (LaVision BioTec)
12:45 - 13:00 Closing statements
Ivan Yudushkin (MFPL)
13:00 - 14:00 Lunch break
14:00 - 16:00 Practical sessions (by appointment)
  • MicroTime 200 (FLIM/FCS/Anisotropy)
  • DeltaVision OMX (3D structured illumination)
  • Light-sheet
  • Brillouin scattering confocal microscopy
  • Choice of fluorophores and sample preparation
16:00 - 16:30 Coffee break and poster presentation
16:30 - 18:30 Practical sessions (by appointment)
  • MicroTime 200 (FLIM/FCS/Anisotropy)
  • DeltaVision OMX (3D structured illumination)
  • Light-sheet
  • Brillouin scattering confocal microscopy
  • Choice of fluorophores and sample preparation

Archive

The following international workshops or courses have been organized by PicoQuant GmbH along with a local research institute in the recent years.

Seminar on the MicroTime 200

August 11, 2017 | Tianjin, China


Science in your labs - St. Louis 2016

December 2, 2016 | St. Louis, MO, USA


Science in your labs - Moscow 2016

October 12, 2016 | Moscow, Russia


FluoTime 300 Roadshow in Poland 2016

May 30 - June 3, 2016 | Krakow, Poland


FluoTime 300 Roadshow in China 2016

May 11, 2016 | Wuhan, China


Science in your labs - Daegu 2015

July 6, 2015 | Daegu, South Korea


FluoTime 300 Roadshow in Poland

May 26 - 29, 2015 | Chorzow, Wroclaw, Poland


Science in your labs - Mexico 2015

May 18 - 20, 2015 | Mexico City/Cuernavaca/San Luis Potosi, Mexico


Science in your labs - São Paulo 2015

April 17, 2015 | São Paulo, Brazil


Science in your labs - Lausanne 2015

March 17 - 18, 2015 | Lausanne, Switzerland


Science in your labs - St. Louis 2014

December 11, 2014 | St. Louis, MO, USA


Science in your labs - Vienna 2014

November 18 - 19, 2014 | Vienna, Austria


Science in your labs - Wuhan 2014

June 18, 2014 | Wuhan, China



Science in your labs - St. Louis 2013

December 12, 2013 | St. Louis, MO, USA


Science in your labs - Vienna 2013

November 12 - 13, 2013 | Vienna, Austria


Science in your labs - Liverpool

October 11, 2013 | Liverpool, United Kingdom


Science in your labs - San Diego

June 10, 2013 | San Diego, USA


Mini-Symposium on Fluorescence Spectroscopy

September 28, 2012 | Houston, USA


South-East Asian Course on F-techniques

July 16 - 20, 2012 | Singapore, Singapore


Science in your labs - Vienna

June 19 - 20, 2012 | Vienna, Austria


Workshop on advanced fluorescence spectroscopy

January 19, 2012 | Los Angeles, USA


South-East Asian Course on F-techniques

June 20 - 24, 2011 | Singapore, Singapore


Mini-Symposium on Fluorescence Spectroscopy

May 27, 2011 | Munich, Germany


PicoQuant Roadshow in China

May 9 - 19, 2011 | China


Mini-Symposium on Fluorescence Spectroscopy

March 4, 2011 | Baltimore, USA


Workshop on advanced fluorescence spectroscopy

January 10, 2011 | Los Angeles, USA


Single Molecule Fluorescence Workshop

April 26 - 28, 2010 | BNL, USA


Single Molecule Fluorescence Workshop

January 22 - 23, 2009 | Sacramento, USA


Single Molecule Fluorescence Workshop

January 17 - 18, 2008 | Sacramento, USA



Single Molecule Fluorescence Workshop

January 18 - 19, 2007 | Sacramento, USA


Single Molecule Fluorescence Workshop

January 19 - 20, 2006 | Sacramento, USA


 

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