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Fluorescence Course

14th European short course on
“Principles and Applications of Time-resolved Fluorescence Spectroscopy”

November 7-10, 2016 in Berlin, Germany

Image Fluorescence Course 2016

Logo CFSThis course was held in cooperation with Prof. J.R. Lakowicz from the Center for Fluorescence Spectroscopy & the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA

Summary

During the 14th edition of the "European Short Course on Time-resolved Fluorescence", 40 participants from around the world got an in-depth introduction to time-resolved spectroscopy and microscopy in the field of Life Sciences through a series of lectures and hands-on experiments. The course was complemented by a welcoming reception on the first day and a dinner on the third day, giving the attendees the opportunity for meeting the lecturers and networking in an informal atmosphere.

Group picture

Course organisation

The course opened with a lecture on the principles of fluorescence spectroscopy by Joseph R. Lakowicz and was followed by lectures by Zygmunt “Karol” Gryczynski and Senior Scientists of PicoQuant covering topics like instrumentation for time-resolved fluorescence spectroscopy and microscopy, anisotropy, solvent effects, quenching, Förster Resonance Energy Transfer (FRET) and applications of fluorescence in Life and Material Sciences.

The practical hands-on sessions were led by application specialists from PicoQuant and Agilent. Each experimental station focused on a specific fluorescence method such as anisotropy, steady-state and time-resolved fluorescence spectroscopy, and data analysis. A short demonstration of Fluorescence Lifetime Imaging (FLIM) and STED super-resolution was also offered. The instruments presented included steady-state (Cary Eclipse, Agilent) and time-resolved spectrometers (FluoTime 200 and FluoTime 300, PicoQuant) as well as a time-resolved super-resolution microscope MicroTime 200 STED from PicoQuant.

Hands-on instrumentation

The following companies offered hands-on experiments during the course:

  • Agilent
  • PicoQuant

Course instructors

  • Joseph R. Lakowicz, Professor of Biochemistry and Director of the Center for Fluorescence Spectroscopy
  • Zygmunt "Karol" Gryczynski, Professor at Department of Physics and Astronomy at the Texas Christian University
  • Rainer Erdmann, Managing Director of the R&D company PicoQuant GmbH and specialist in ultrasensitive fluorescence analysis
  • Felix Koberling, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence microscopy applications and instrumentation
  • Christina Litwinski, Senior Scientist at PicoQuant GmbH and specialist in time-resolved fluorescence spectroscopy
  • Matthias Patting, Senior Scientist at PicoQuant GmbH and specialist in software design for Time-Correlated Single Photon Counting (TCSPC) instrumentation
  • Michael Wahl, Senior Scientist at PicoQuant GmbH and specialist in instrumentation for Time-Correlated Single Photon Counting (TCSPC)

Time schedule

 

Monday,
November 7
Tuesday,
November 8
Wednesday,
November 9
Thursday,
November 10
8:00
Registration
9:00
Joseph R. Lakowicz
Biophysical Applications and Time-resolved Fluorescence
9:00
Joseph R. Lakowicz
Advanced Topic: Plasmon-controlled Fluorescence
9:00
Felix Koberling
Introduction to Fluorescence Microscopy Techniques

8:45

Welcome and Introduction

10:30
Coffee Break
10:30
Coffee Break
10:45
Change Buildings and Coffee Break

9:00
Joseph R. Lakowicz
Basic Definitions and Principles of Fluorescence

 

11:00
Michael Wahl
Instrumentation for Time-resolved Fluorescence Measurements (2)
11:00
Matthias Patting
Computational Aspects of TCSPC Data Analysis
11:15
Practical Sessions (5)
10:30
Coffee Break
12:30
Lunch Break
12:30
Lunch Break
12:45
Lunch Break and End of Course

11:00
Joseph R. Lakowicz
Time-resolved Fluorescence and Fluorescence Probes

13:30
Matthias Patting
Practical TCSPC Data Analysis
13:30
Zygmunt “Karol” Gryczynski
Analytical Applications of Fluorescence
 
12:30
Group Picture
14:00    
Christian Litwinski
Introduction to Practical Sessions
15:00
Change Buildings
 

12:45
Lunch Break

14:30
Change Buildings
15:15
Practical Sessions (3)
 
13:45
Rainer Erdmann
Instrumentation for Time-Resolved Fluorescence Measurements (1)
14:45
Practical Sessions (1)
16:45
Coffee Break
 

15:15
Coffee Break

16:15
Coffee Break
17:00 - 18:30
Practical Sessions (4)
 

15:45 - 17:30
Zygmunt “Karol” Gryczynski
Fluorescence Measurements, Probes & Standards

16:30 - 18:00
Practical Sessions (2)
19:45
Course Dinner
 
18:30
Opening Reception
18:00
Spare Time
   

Workshop impressions

 

Archive

The European short course on "Principles and Applications of Time-resolved Fluorescence Spectroscopy" is an annual event since 2003. For further details about each year's event, please select the year from the list below.

 

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